Download GDP iPCR protocols Word Doc file (Updated 19-Sep-2006)

Transposons used in this screen:

• BG lines - P{GT1}
• KG lines - P{SUPor-P}
• EY lines - P{EPgy2}
• MB lines - Mi{3xP3-EGFP.ET1}

---

BG lines - P{GT1}

• Reference for this P element: Lukacsovich et al. (2001) Genetics 157:727. [Abstract]
• Sequence
• Genetic background used during the screen: w¹¹¹⁸
• Transposase: Δ2-3
• Marker for selection: w⁺ eye color

 

---

KG lines - P{SUPor-P}

• Reference for this P element: Roseman et al. (1995) Genetics 141:1061. [Abstract]
• Sequence - FlyBase-compiled
• Genetic background used during the screen: y¹; ry⁵⁰⁶
• Transposase: Δ2-3
• Marker for selection: w⁺ eye color or y⁺ body color

 

---

EY lines - P{EPgy2}

• Reference for this P element: Bellen et al. (2004) Genetics 167:761-781. [Abstract]
• Sequence
• Genetic background used during the screen: y¹ w^67c23
• Transposase: Δ2-3
• Marker for selection: w⁺ eye color or y⁺ body color

 

---

MB lines - Mi{3xP3-EGFP.ET1}

 

• Reference for this transposable element: Metaxakis et al. (2005) Genetics 171:571. [Abstract]
• Sequence
• Genetic background used during the screen: w¹¹¹⁸ (isogenized)
• Transposase: Minos
• Marker for selection: GFP
• Can plasmid rescue