MiMIC RMCE Lines

MiMIC RMCE Lines

The Bellen Lab has recently generated a collection of protein trap lines containing the EGFP-FlAsH-Strep-TEV-3xFlag tag using the RMCE technique. Please visit the MiMIC techniques page for a description to the method for introducing this reporter into MiMIC insertion strains.

Reference: Nagarkar-Jaiswal S, Lee PT, Campbell ME, Chen K, Anguiano-Zarate S, Cantu Gutierrez M, Busby T, Lin WW, He Y, Schulze KL, Booth BW, Evans-Holm M, Venken KJ, Levis RW, Spradling AC, Hoskins RA, Bellen HJ (2015) A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila. eLife 4:05338. [Link]

** We have now developed a method to do this genetically. See crossing schemes page for details. **

Reference: Nagarkar-Jaiswal S, DeLuca SZ, Lee PT, Lin WW, Pan H, Zuo Z, Lv J, Spradling AC, Bellen HJ (2015) A genetic toolkit for tagging intronic MiMIC containing genes. eLife 4:e08469. [Link]

Note: The pictures shown here are immunostainings with anti-GFP antibodies imaged with a confocal microscope. We primarily screened 3rd instar larval nervous system for EGFP expression.

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Line Gene(s) Targeted Phase BDSC Stock
MI00012Grd060285
MI00031Sema-1a260140
MI00051dpn059755
MI00053Rfx139645
MI00053Rfx139644
MI00053Rfx139646
MI00053Rfx139643
MI00053Rfx139642
MI00053Rfx142207
MI00063
(by cross)
SCAP064463
MI00075
(by cross)
CG300902
MI00099nAChRalpha1060141
MI00103
(by cross)
Usp15-31064430
MI00107CG31030059756
MI00111Msp300059757
MI00116Tep2161650
MI00120CG7458060142
MI00126rdgC160143
MI00135aret160144
MI00139mbl059758
MI00149side160507
MI00153LpR1160145
MI00155p130CAS060508
MI00176CG42732261746
MI00178Ac76E159400
MI00191beat-Va160146
MI00193
(by cross)
osp0
MI00204
(by cross)
Aldh-III2
MI00209nkd159759
MI00245cv-c061805
MI00261Efa6060147
MI00265vib259760
MI00269Roc2160509
MI00290tutl139651
MI00290tutl139652
MI00290tutl139653
MI00296rhea039649
MI00296rhea039650
MI00296rhea039647
MI00296rhea039648
MI00302Pli261747
MI00315Gp210161651
MI00316
(by cross)
CG6024164464
MI00332CG3339260148
MI00340gish063151
MI00344CG9727260511
MI00347Abl159761
MI00377Rm62261748
MI00380comm160286
MI00386bol164431
MI00393CadN039639
MI00393CadN039638
MI00393CadN039640
MI00393CadN039641
MI00406hdc259762
MI00407PP2A-B'161749
MI00413Eip63E159763
MI00430
(by cross)
CG88510
MI00438jumu059764
MI00441
(by cross)
CG31694 0
MI00446Shal160149
MI00453nAChRalpha1060150
MI00458luna060151
MI004595-HT2A259765
MI00479klar061652
MI00480sima260152
MI00486Mob2260153
MI00489Mmp2260512
MI00493foxo059766
MI00494wnd239656
MI00494wnd239654
MI00494wnd239655
MI00494wnd239657
MI00615sba160513
MI00709Frq1060284
MI00773MRP160154
MI00808Dscam3160155
MI00813
(by cross)
CG11241164470
MI00833Galphao160504
MI00848Shab160514
MI00874bnl161653
MI00909Alg-2059767
MI00919Jupiter260156
MI00929Ac3260157
MI00946Lon059286
MI00949ETHR260158
MI00957GlyP060159
MI00963
(by cross)
CG42732263152
MI00969CASK059768
MI00977msi261750
MI00986CG32698161751
MI00994CG32638060160
MI00996
(by cross)
CG17349163153
MI01025trbl261654
MI01052CG34114160515
MI01102svp163154
MI01118Nep2260161
MI01128fry159769
MI01139CG34113160162
MI011405-HT1A261752
MI01144Ac78C059401
MI01169
(by cross)
CG10086163155
MI01180Atox1160163
MI01181scramb1060164
MI01186wry161753
MI01190
(by cross)
CG32413063156
MI01202AdoR160165
MI01205pyd160166
MI01213Men061754
MI01217bon059770
MI01256Baldspot259771
MI01299Tep2259402
MI01307p53060516
MI01332
(by cross)
SLC5A110
MI01347bi259290
MI01358dpr6159287
MI01402Indy060167
MI01408dpr15161655
MI01410CG14764160168
MI01427RhoGEF3161755
MI01427
(by cross)
RhoGEF3263157
MI01467beat-Ic159772
MI01473Ptp61F061756
MI01483psq059773
MI01492Pxn259774
MI01496shn159403
MI01498sdk160169
MI01511Ptp99A061757
MI01532Pdp1159775
MI01549bs059776
MI01552ed159777
MI01568
(by cross)
stops263158
MI01568
(by cross)
Gycbeta100B263159
MI01574
(by cross)
CG34384063160
MI01598Dscam4161758
MI01603
(by cross)
CG30497064432
MI01630nrm160505
MI01635bnl060170
MI01638ImpL2059778
MI01646
(by cross)
CG18150
MI01660
(by cross)
CG91090
MI01684dysc060517
MI01695dpr12160171
MI01722CG4587059288
MI01722CG4587259289
MI01727siz260287
MI01735Shaw160518
MI01743dpr11160519
MI01759step061759
MI01785nAChRalpha6160172
MI01790
(by cross)
MTA1-like159415
MI01791for159779
MI01803cdi261760
MI01815CG42321161761
MI01820fz059780
MI01824neur159781
MI01835plum160520
MI01841RYa-R060521
MI01843RYa-R060173
MI01874
(by cross)
eys263162
MI01893Dys059782
MI01905
(by cross)
ASPP0
MI01907Gprk2159783
MI01909GluRIIE160174
MI01920CG315590
MI01930GABA-B-R1060522
MI01935
(by cross)
CG331160
MI01941brm159784
MI01958
(by cross)
Lpin0
MI01991
(by cross)
CG51910
MI02001CG31183160175
MI02003
(by cross)
ptc0
MI02025Hmx259785
MI02031DIP-alpha160523
MI02041CG46121 060176
MI02059Nlg1159786
MI02072CG9747160177
MI02091beat-VII260178
MI02101Syp161762
MI02112
(by cross)
CG5830163163
MI02135klg159787
MI02141
(by cross)
hbs1
MI02153
(by cross)
Mondo0
MI02168shakB260524
MI02189
(by cross)
Sur0
MI02197Syt1059788
MI02198Unc-89060525
MI02201dpr1160179
MI02206nAChRbeta2059789
MI02221
(by cross)
CG10426263164
MI02222Glut1059607
MI02225
(by cross)
neb0
MI02243GLaz060526
MI02248
(by cross)
CG14669064433
MI02257
(by cross)
toc264465
MI02265fry260180
MI02272Antp059790
MI02290igl160527
MI02292osa059791
MI02300crc159608
MI02321Dscam11
MI02376sif259792
MI02379bru-3060181
MI02380
(by cross)
CG32052164434
MI02407brat159793
MI02424CG2201061763
MI02427pnt160528
MI02457CG5853162348
MI02461fas159794
MI02467
(by cross)
sfl263165
MI02479
(by cross)
rols164471
MI02485Elk160182
MI02509Cep135260183
MI02518
(by cross)
zip0
MI02522Atg18b159404
MI02527beat-Ib160184
MI02530dpr2160185
MI02542Unc-13-4B060186
MI02556Lk6159795
MI02577alpha-Cat059405
MI02578SerT160529
MI02592
(by cross)
Ggamma30A2
MI02620Rdl059796
MI02621CG32085161764
MI02654Mkp3160530
MI02662bbg160187
MI02663
(by cross)
geko164435
MI02729dysc059797
MI02741Trl160188
MI02742gce160189
MI02765
(by cross)
loh063166
MI02777
(by cross)
CG6356263167
MI02784CLIP-190160190
MI02791CG33543160531
MI02796CG15630160532
MI02844Ten-m159798
MI02852Egfr161765
MI02853
(by cross)
CG33158164436
MI02890GluClalpha260533
MI02899
(by cross)
Pde110
MI02911bun061806
MI02915Ask1259799
MI02928rdx159800
MI02966Acsl259291
MI02986Btk29A161656
MI02987brp059292
MI03001sns159801
MI03008CG11221160191
MI03050dsx160192
MI03075
(by cross)
esn2
MI03085Dop1R1259802
MI03101nmo261766
MI03102beat-IIb059406
MI03132Ptp61F061767
MI03134kst160193
MI03164Pkn060194
MI03173
(by cross)
Vha68-30
MI03191DIP-theta159803
MI03208fred159804
MI03218
(by cross)
CG55351
MI03223nAChRalpha4059805
MI03229timeout159806
MI03249CG34353060534
MI03270
(by cross)
sas1
MI03354nrv2159407
MI03375Frl060195
MI03381mRpS9060196
MI03390
(by cross)
CG7139063168
MI03400CG11873161768
MI03404-5CCAP-R060197
MI03410tacc060198
MI03415dnc260535
MI03440tau060199
MI03470Rim060200
MI03490Fur1060536
MI03491
(by cross)
Hcs064437
MI03522
(by cross)
Drep10
MI03557dpr10159807
MI03572jdp060201
MI03592shep061769
MI03594dpr9160202
MI03598
(by cross)
CG31637264438
MI03631GluRIIB261770
MI03652trio059808
MI03653Mical060203
MI03665Eip75B160204
MI03674Fas3159809
MI03679CCKLR-17D1261771
MI03683Herp260537
MI03687bru-2161772
MI03726beat-IIIc159810
MI03727
(by cross)
NKAIN263169
MI03754CG9849260205
MI03763Nlg1060206
MI03803arr159811
MI03814mam059812
MI03815Ance-3260207
MI03825
(by cross)
sli264472
MI03836
(by cross)
scb263170
MI03838
(by cross)
DIP-zeta063171
MI03851CG423880
MI03906drongo259408
MI03941Mhc159813
MI03943
(by cross)
zip063172
MI03969beat-VI061773
MI04001fbl060538
MI04002Cnx99A059293
MI04004
(by cross)
CG17646064439
MI04029
(by cross)
CG426632
MI04030heph059814
MI04033sm159815
MI04076Pex10059816
MI04090CG10345160539
MI04094
(by cross)
CG115762
MI04101
(by cross)
Rgk3163173
MI04110CG10226060208
MI04181
(by cross)
Pvr0
MI04189Piezo060209
MI04217dlp160540
MI04263
(by cross)
NijC064440
MI04265
(by cross)
CG431431
MI04279NaCP60E060210
MI04295robo2161774
MI04308
(by cross)
Fs1
MI04411Ten-a260541
MI04413slo260542
MI04472CG34357161775
MI04507Pka-C3160211
MI04508ChAT060288
MI04520
(by cross)
CG31522064441
MI04522Cat060212
MI04530
(by cross)
Fkbp142
MI04563NetA159409
MI04569DAAM060213
MI04570Camta160543
MI04580trol060214
MI04585
(by cross)
Trpm264467
MI04607htt160215
MI04611TfAP-2061776
MI04671
(by cross)
CG8745164442
MI04674unc79160216
MI04696Nckx30C059294
MI04712Hs6st060217
MI04721
(by cross)
CG34371163174
MI04730pdgy060218
MI04745LpR2160219
MI04746
(by cross)
kcc2
MI04761orb059817
MI04763
(by cross)
IP3K1064473
MI04772
(by cross)
CG43897264468
MI04825pum059818
MI04831cert060544
MI04834
(by cross)
sca264443
MI04840
(by cross)
CG11537064444
MI04842rst159410
MI04868Dl159819
MI04897pxb060220
MI04913Nhe2260545
MI04922Dad161777
MI04958
(by cross)
CG343701
MI04965CG12484159820
MI04979VGlut259411
MI04996Unc-115b059295
MI05000
(by cross)
apt0
MI05027Lrch160221
MI05100Ndae1261778
MI05111sima059821
MI05111sima260222
MI05145
(by cross)
oys0
MI05166Mob2161779
MI05200Eip93F159412
MI05212Or47b260546
MI052135-HT1B060223
MI05229kel260224
MI05248
(by cross)
tim264469
MI05253rg060225
MI05262CG14207260226
MI05281Mekk1260227
MI05295Dif160506
MI05296Pkc53E059413
MI05302Trpm159822
MI05320EcR159823
MI05333
(by cross)
kay063175
MI05334Ser059824
MI05339Neto261780
MI05371unc-5160547
MI05376SIFaR160228
MI05382crb061781
MI05387RhoGAP100F059414
MI05391
(by cross)
CG433330
MI05423ClC-a059296
MI05435
(by cross)
nwk164445
MI05449Tnks160229
MI05488TrissinR261782
MI05512Treh259825
MI05524
(by cross)
CngA0
MI05533WASp060289
MI05541Hnf4160230
MI05542CG42235060231
MI05545
(by cross)
Gyc32E0
MI05549nAChRalpha5059826
MI05577dpr13060232
MI05586
(by cross)
eIF5B064446
MI05598Atpalpha059297
MI05602Sema-2a060548
MI05636Kank260233
MI05646spir059827
MI05689vn160234
MI05715beat-IV1
MI05737
(by cross)
capu0
MI05739
(by cross)
CG14516164447
MI05741CG1632060235
MI05748Ance159828
MI05774hig159829
MI05781SPoCk159830
MI05788TkR86C260549
MI05804
(by cross)
CCAP-R163176
MI05807Octbeta1R260236
MI05822uif161783
MI05859luna059831
MI05901Imp260237
MI05906CG32333260238
MI05926CG43373060239
MI05929Or65a260240
MI05938cindr160241
MI05955CG31089060242
MI05963dpr3160243
MI05984PhKgamma061784
MI06026Nc73EF159416
MI06036CG16779061785
MI06045
(by cross)
Lip41
MI06062C3G160244
MI06118tok160550
MI06143CG31871060551
MI06179DCX-EMAP061786
MI06181robo3060290
MI06217Octbeta3R160245
MI06245
(by cross)
Epac0
MI06252mas160552
MI06255Nlg4160246
MI06288
(by cross)
Alh063177
MI06304
(by cross)
CG110440
MI06307Trpml259832
MI06332Slob260247
MI06350fru161787
MI06353dlg1059417
MI06376LRP1160248
MI06408mgl160249
MI06413Syp159298
MI06414nord260250
MI06441CrebA059833
MI06452
(by cross)
hid1
MI065005-HT2B160251
MI06502AP-2alpha159834
MI06507
(by cross)
Cad86C263178
MI06526CG30463060252
MI06550
(by cross)
SRPK1
MI06587
(by cross)
Galphao263179
MI06633ttv060253
MI06644CG1090160254
MI06667rogdi059299
MI06684fra159835
MI06697
(by cross)
CG32772
MI06703Hers160255
MI06777
(by cross)
CG42321163180
MI06778dpr8061788
MI06779Nep5160256
MI06792CG42674060257
MI06794CG344111
MI06811
(by cross)
Roc21
MI06811
(by cross)
Tret1-12
MI06823Pdk1059836
MI06828
(by cross)
CG62870
MI06830Cdep161789
MI06860spri260258
MI06880crp059837
MI06892CRMP059300
MI06928larp161790
MI06935
(by cross)
Pka-R10
MI06957comm3161791
MI06971Ccn160259
MI07022Sh260553
MI07056Atg1059301
MI07056CG427092
MI07079CAH2159418
MI07099Fim059838
MI07114
(by cross)
Alp1 163181
MI07172Mgstl061792
MI07187Pura060554
MI07215unc-104159302
MI07274Glut4EF260555
MI07277
(by cross)
CG426720
MI07289cact060556
MI07302CG30438161793
MI07336
(by cross)
twin163182
MI07345Ace160260
MI07356
(by cross)
veil0
MI07398
(by cross)
stai1
MI07480cher060261
MI07492Cyp313a3160557
MI07502tmod059303
MI07575
(by cross)
l(2)gl063183
MI07577Snoo061794
MI07633stas260262
MI07645TrpA1061795
MI07659
(by cross)
drpr163184
MI07680Vmat060263
MI07695
(by cross)
btsz164448
MI07729A2bp1061657
MI07745pbl259839
MI07746ZnT35C159419
MI07766
(by cross)
Nedd4063185
MI07786CG6454160264
MI07821spen259840
MI07890
(by cross)
CG33510163186
MI07916
(by cross)
Men-b263187
MI07920Ptp4E061796
MI07934
(by cross)
Mi-2163188
MI07948DIP-eta161797
MI08022Rgk1060265
MI08022CG34045161798
MI08056Ptpmeg061799
MI08149Eb1059420
MI08161mub0
MI08287DIP-delta160558
MI08316shi259421
MI08383cnn260266
MI08426alphaTub85E060267
MI08433CG438670
MI08439Cad99C160268
MI08468
(by cross)
CG42324163189
MI08481
(by cross)
Kdm2263190
MI08482beat-Vc160269
MI08496EDTP060270
MI08510
(by cross)
CG56740
MI08520
(by cross)
PAPLA1163191
MI08542SKIP060559
MI08547trpl060271
MI08564CG73781
MI08565Ca-alpha1T061800
MI08626
(by cross)
Lip1063192
MI08641pdm3260560
MI08672Prosap059422
MI08675arm160561
MI08698
(by cross)
Sam-S163193
MI08707dpr17161801
MI08774form3260562
MI08818qless059841
MI08838CG42321260563
MI08884CG14024160564
MI08892Gycbeta100B260565
MI09145
(by cross)
Pka-R21
MI09168CG9492160272
MI09216
(by cross)
tws163194
MI09277Gad1259304
MI09281Nmdar2260566
MI09284
(by cross)
CG423992
MI09308PKD060273
MI09316Mp060567
MI09330aPKC160274
MI09362CG9743160275
MI09401Dop2R060276
MI09447
(by cross)
Pde1c263195
MI09505nolo260568
MI09551CG6767259305
MI09552ADD1160569
MI09643nuf261802
MI09647GLS060277
MI09718Nos060278
MI09722RanBPM2
MI09783unk259842
MI09826wun060279
MI09857AP-1gamma2
MI09878Meltrin060570
MI09888
(by cross)
CG9541163196
MI09912
(by cross)
Tsp42Ee063197
MI10086Ptp10D061803
MI10095Cyp28d2260280
MI10126
(by cross)
CG13248163198
MI10140ck061804
MI10157FeCH260571
MI10467NetB1
MI10467CG430880
MI10477
(by cross)
CycA064269
MI10513
(by cross)
Nipped-A0
MI10547b060281
MI10594
(by cross)
Hrb98DE163199
MI10698
(by cross)
a163200
MI10748ndl160282
MI10770
(by cross)
Wdr62 0
MI10787
(by cross)
Sdc0
MI10885Sh259423
MI10910
(by cross)
CG316782
MI10931mnb1
MI10938KCNQ2
MI11015
(by cross)
Sap-r263201
MI11060
(by cross)
puc164449
MI11080
(by cross)
Vha44263202
MI11086
(by cross)
CG14085263203
MI11129app060283
MI11167Ca-alpha1D2
MI11275
(by cross)
mfas263204
MI11328
(by cross)
Tsp42Ec0
MI11538
(by cross)
drk264450
MI11749
(by cross)
Taf1164451
MI11750
(by cross)
Hipk1
MI11796
(by cross)
Nmdar1063205
MI11971sgg1
MI12013
(by cross)
pcs0
MI12194
(by cross)
CG3764263206
MI12277
(by cross)
par-1264452
MI12699
(by cross)
TyrRII2
MI13336
(by cross)
mod(mdg4)1
MI13909
(by cross)
CG422611
MI13970
(by cross)
pes0
MI14130
(by cross)
lqfR1
MI14289
(by cross)
CG135510
MI14361
(by cross)
Muc91C1
MI14452
(by cross)
CG331440
MI14614
(by cross)
CG300470
MI14762
(by cross)
ctrip1
MI14951
(by cross)
Ets96B164453
MI15336
(by cross)
Sik30
MI15372
(by cross)
egr0
MI15397
(by cross)
Ndg0
MI15497
(by cross)
AdSS1
MI15528
(by cross)
dpr16164454
MI15552
(by cross)
Cyp4aa10
MI15736
(by cross)
ppan1