The Bellen Lab has recently generated a collection of protein trap lines containing the EGFP-FlAsH-Strep-TEV-3xFlag tag using the RMCE technique. Please visit the MiMIC techniques page for a description to the method for introducing this reporter into MiMIC insertion strains.
Reference: Nagarkar-Jaiswal S, Lee PT, Campbell ME, Chen K, Anguiano-Zarate S, Cantu Gutierrez M, Busby T, Lin WW, He Y, Schulze KL, Booth BW, Evans-Holm M, Venken KJ, Levis RW, Spradling AC, Hoskins RA, Bellen HJ (2015) A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila. eLife 4:05338. [Link]
** We have now developed a method to do this genetically. See crossing schemes page for details. **
Reference: Nagarkar-Jaiswal S, DeLuca SZ, Lee PT, Lin WW, Pan H, Zuo Z, Lv J, Spradling AC, Bellen HJ (2015) A genetic toolkit for tagging intronic MiMIC containing genes. eLife 4:e08469. [Link]
Note: The pictures shown here are immunostainings with anti-GFP antibodies imaged with a confocal microscope. We primarily screened 3rd instar larval nervous system for EGFP expression.
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