GDP Screen Database

This is the public gateway to the ongoing Gene Disruption Project of the Bellen/Hoskins/Spradling labs.

Order page – only possible for lines that are not yet sent to the Bloomington Stock Center
Download GDP iPCR protocols PDF file (Updated 05-May-2011)
Transposons used • MiMIC constructs

References

Spradling AC, Bellen HJ, Hoskins RA (2011) Drosophila P elements preferentially transpose to replication origins. Proceedings of the National Academy of Sciences USA 108:15948-15953. [PDF] [Suppl]
Supplementary Data to Download:
- ORC Site Names (.xls)
Venken KJT, Schulze KL, Haelterman NA, Pan H, He Y, Evans-Holm M, Carlson JW, Levis RW, Spradling AC, Hoskins RA, Bellen HJ (2011) MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Methods 8:737–743. [PDF] [Suppl]

Bellen HJ, Levis RW, He Y, Carlson JW, Evans-Holm M, Bae E, Kim J, Metaxakis A, Savakis C, Schulze KL, Hoskins RA, Spradling AC (2011) The Drosophila Gene Disruption Project: progress using transposons with distinctive site-specificities. Genetics 188:731-743. [PDF]
Supplementary Data to Download:

Bellen HJ, Levis RW, Liao G, He Y, Carlson JW, Tsang G, Evans-Holm M, Hiesinger PR, Schulze KL, Rubin GM, Hoskins RA, Spradling AC (2004) The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. Genetics 167:761-781. [PDF]

Last update: 2012-04-04

Search Suggestions:

If you are unable to find insertions for your gene using the full CG name or full gene name, try partial name or check FlyBase for synonyms. You may now search with FBgn ID.
Another powerful search to try is the cytological location and narrow by chromosome arm. This will give all insertions available at a given mapping position. Scan the coordinates on the results page to find the insertion closest to your gene.
Click the link of the insertion name of interest on the results page. This will take you to the details page for that line where all information is consolidated, and a link to Gbrowse on FlyBase is provided.

GDP has generated:

BG lines: 485 (P{GT1})
KG lines: 2165 (P{SUPor-P})
EY lines: 3352 (P{EPgy2})
MB lines: 2642 (Mi{ET1})
MI lines: 3597 (Mi{MIC})

QUERY FOR:
Gene / CG / CR (3-4 letter abbrev. or CG/CR number. Try both.)	Do not use wildcards (* or ?).
Mapping Pos. (e.g. 93A) (no wildcards req.)		Chromosome Arm
Search for Line name: BG, KG, MB, etc. (no wildcards req.) - see transposons		FBgn ID

Acknowledgments: This project is a collaboration among the laboratories of Hugo Bellen (Baylor College of Medicine), Roger Hoskins (Lawrence Berkeley National Laboratory) and Allan Spradling (Carnegie Institution of Washington) and is supported by the National Institutes of Health and the Howard Hughes Medical Institute. The flies are generated, selected, balanced and maintained by Yuchun He, Ying Fang, Zhihua Wang and Jianping Li in the Bellen lab. The inverse PCRs and sequencing are performed by Martha Evans-Holm in the Hoskins lab. Mapping information is obtained by Joe Carlson in the Hoskins lab followed by manual curation by Bob Levis and Allan Spradling in the Spradling lab. The web database and strain requests are being handled by Karen Schulze in the Bellen lab.