CR99906

CRIMIC CR99906

Gene Cdk4

CG5072

Information

Donor Location Cytology FBti Stock number
pM142R:16581530..1658156453C9FBti020075481218

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK

Images

Construct Design Sequences

sgRNA sequence
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
TCGGTTTTCGGTTCTCGGAGATAGAAAAGCATAGCGGAGCATATAGCCAGACTTCTTCCATTAGCGCACCTGTCACACCAGTCTTTATCGCCAGTTTTGGGGTTATCTAGAAAAGGCAACAGGTATGAAATCAGGTAAGGCCCTCCCTCTCTCTCTCACGCACTCGCACTCTCCCTCTCACGCTACGATCTGTGTGAACTATCGCGTGGGGACACAAGGACATATCGATACGTGACATGCAGACGATCGATAACCAAAAAAAAAACACCCCTTCACTTCCAGCTGGTGCAACTAACGGTTTCGATTACCCGGAATATTAGAGGAATACGCAAATAACCGTAAAATCCAGCTAAATGTCATATGTACGCCAGCTGAAGCGGCAGAAAATGTCCCAGGCGAAGAAGTTCGGCGATGGCGATCCGTTCAACTACCAGGAGCTCAACATCATCGGCGAAGGTGAGCGACTTGGGTGGGATTTAATAATGGGACGCGGTTGCTTTGTTGTTGGGATAGGTCCGGCTCTCTTCGCACGAGAGGCTTTCGTTTGCTGGTCGCTTGTTGTTAACGTTTGATTACTTTAATTTGCTTACAAAGTCTGGGCTATTTGGTTTCACTACTGTTTACGATCAATCGGGAGCTCCGACATTGTTATGAAGCTGAAATGACAAGTGCACTTGGAGGAATGTTGTTGTTTTTTTACCCCTTTACTAATTAAGCGTGGAAATTCCGCGAAAAGGGGAAATATTGGATGGAAAACTCCCCTGTATTGGATCCTACACCTTCTACCTGCATACTTTTAGGTCCACTCTCGGCGGACTACGTGTTTCTGCCGATCTCACATCCATGATGATCTGACAAGTGGCTCATGGTCTTTTTTTTTTGTGGTCAGTAATTACGATCGCTTGTTTGCTGTAAATAAAGCCCAAATCAATTACGTCACCGATCATGCCGA gagtcatgggtccgcacaatttcatctgttatacaacgcacacacacacacatacactcaaactttcggctgtctgcctttctgtgcacattcattcaattattattcgctttcgctcagtctcatttttgctactttgtactatggaaaatttgaccactttttgtggccaaactaactccaaactttttttgcattgccttacactagcccatatttttaatgatatataatatatattttttagtttgcagatagtcattcaattaacttttttgcattgcctaacagtagtcaccgttgtaactaaatgcatgcatttaatattacatatattaatcggttttcggccagataaagtgcgtaaatttcccatagtgctttgctttgttgttttctttgcgcaatttttcgagtttattttgcttgtatATTTGTATATTTTTTTGACAATTCCAATTGGACAGACCAGTTGCCGACGTTCAAAAACGAGTCGAGCTCACGGCCTTGTACTTGAATGTAGCAACAGCGGCACAGTTCATAAGCCAGGTGGTCTAGAGACGGTGACAAAGGGACGGAAAACCAAATTCACCCCCCCCCCCCCTCAAGTTGCGTTTATTTCGGGTGTATCAGTACATATCTGCTGTTTCTGTATGTCAGTGTCAACCGAAATAGCCGGTGCCTTCTAGCTTTTCTACCCCCCACGCATGTCCGTCCGTCCGTCCGTTTGTCCATTTGTCCATTTGTCCGTACGGGTGATAAATTTTCGGTGACATTTCTCTGGCATGCCAGAATTTGATTCTGATTGGGAATTTTGCTCGTCTCTTGTCGTTTTTCTTTGCCCGTGAACATGTAGAAATTAAGATCACGTTGTACCTGAGACAGCAACAGTTTCATGAGGAAATTAGTCTGAATTTCGTGCAATATTTTGACTAATTTTGAGTGCATATAATATAGTCTCTAATATATGCAATTATTGCATGCATTGCAATACGATTCTTTTGGGCTAAATGCATTCATTGTCTATTCATTCAATCATTATAAACAGTTAGTTCATCGAACAAGGCAAATAAATCAAGTCACTTCAATCGTAAGGGCTATAAATACGATGTTGAATATGAAAAGTATAAATACACCATAATGATATGAAGAAGCTAAGTGGCACCTTCTCTCTTTCTCTC
 

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