CR99905

CRIMIC CR99905

Gene CalpA

CG7563

Information

Donor Location Cytology FBti Stock number
pM142R:19428635..1942870356D5FBti019948178997

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CTTCCATTTCCGCTTTTGG TAAATTAGAACCACATTCACATTACC

Images

Construct Design Sequences

sgRNA sequence
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
TAACCAAGGTTTGTTCGGAAAATTGCTATGATAAGAACATTTTATTAATTAATCTTATGTCAACAGGTTTGCCTTATTGACATAGTAACGCCAAATCGTCAGGGAAAGATCCCTATGATAAGAATGCGAAATCCTTGGGGCAATGAAGCCGAGTGGAATGGACCATGGAGTGATAGCTCACCAGAATGGCGCTATATACCCGAGGAACAGAAGGCGGAGATTGGACTAACATTCGACAGAGACGGAGAGTTCTGGATGTCCTTCCAAGATTTTCTTAACCACTTCGATCGCGTGGAGGTAGGACTATTAATATTTCTTGGTGAAATATTTCATGATTCATTCGCCATGATTTTGTTCAGATTTGCAATTTGTCGCCGGACTCACTGACCGAGGACCAACAAAACAGTGGCAAGCGAAAGTGGGAGATGTCCATGTATGAGGGAGAATGGACACCCGGTGTTACAGCTGGTGGTTGCCGCAATTTCCTGGATACCTTCTGGCACAACCCCCAGTATATTATCACCCTAGTCGATCCTGATGAGGAAGACGAAGAGGGACAGTGCACCGTCATCGTGGCCTTAATGCAAAAGAATCGCAGATCGAAGCGCAATATGGGAATGGAGTGTCTGACCATTGGTTTCGCTATCTATAGCCTTAATGATCGCGAACTGGAAAACCGTCCCCAGGGTCTGAACTTTTTCAGATACAAGTCTTCTGTGGGAAGATCGCCGCACTTTATCAACACCCGCGAAGTACGTATACGGATATATCCAGGATATGGCTGCAATCACTTAACATATGCGTTTCTTATTTAGGTGTGTGCTCGCTTCAAATTGCCTCCTGGTCACTACCTGATTGTGCCTTCAACTTTCGATCCAAACGAGGAGGGAGAGTTCATCATTCGAGTGTTCTCAGAAACTCAAAATAACATGGAGTGCGTACAAGTAGACAATGACAATGAGTTTGTATATTAGAAATAATGAGGGGTTTCTATAACTAAAAGCTTCTTACGTGCAATATGTGGT CCTCTACACATTCCTGACAGATAAGTTGGTTATGCCATTTTTTAATCACTCCTCTTATATATATTTTTACCTGAGATTAACGCCTGCAGTATTCCAGAAACTCGAAATATATTTCTTCTAGATAAATTCAAATGTAATGTCGGTCTGTCTGTTACATGTTAAGAATAATTCTGATCAATATGAAGTTCAATTTTCTTAATTTATCAGTTATAAAAAGGGTGGCGGCAGCAACATAGTGAAATCTATTAACAAAGTTAAATTTTTCGGTTTATTTTTAACCCTTGTTTAAATTGTATTTTTGTCATGTAACGAGATATTTATTAAAGTAATAATAAAGGAAACTTCCACAACAATATACTTTTAAAAATGGACCCCAACAAATTAAGCTCATCTTGAAAATCTGCTTCTCAATACTCATTAGGCCATTCGAAATCCTTGTACTGTAAATTGTATTGTATTCCATTTGTTTCTCTATAAACGAATTGTTTTCCATTCATTTCAATATCAATCATTTCGCTTTTCACTCCCATCTAGAGAGAATGATGATCATGTGGGTTATGGCGGCAAAGCCGATACGGTAAGTTAACTGTGGTCAATGGGATCGGGATCGCTTATTCAGAAATTTCGCTTTAAAGATTACGCCAGGATTCCCAACACCCAAGCCCATCGATCCTCAGAAGGAGGGCTTGCGACGCCTCTTCGACAGCATTGCCGGCAAGGACATGGAGGTGGATTGGATGGAATTGAAACGCATTTTGGACCATTCGATGAGGGACGGTAAGGCTCGAGATTAAAAACTACTCAAATGGCTTTTCAAACAACGATTTCCTTTCAGATTTACCCAAACCAGTGGTTTTCAACCGTTTCTCCAATAACATGGCCTTTGAGACCCAGGCTGCTGGCCCTGGTGACGATGGAGCTGGCGCTTGTGGACTTTTATCCTTGATTTGTGGACCG
 

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