CR92265

CRIMIC CR92265

Gene Jarid2

Information

Donor Location Cytology FBti Stock number
pM37-p23L:9477038..948653067B9-67B10Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band long band CATCCTGGTCGAACGTACACAGTA TTCCTTCGTGCTGGCACGATTAGA

Images

Construct Design Sequences

sgRNA sequence TGCGAAACACCCCTTCGTCGAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCGCCATGACACAAAGCAAA CAACAACGCCACGACACG GGGTGTTTCGCATCCGTC GGAGTAGGATTTCGCGAGGG
 
Amplicon 5' Amplicon 3'
CCGCCATGACACAAAGCAAAAGCGGTGTAAGAAGAAGCAGAAGACGAAAAAAATATGTGCAAGCAAATCTCGCGAAAGAAAATGAAATTTGCAGCACTTGTCGTGTGTGAAATGTGAACACAGAGTGCACTAAACAATAACAAGACAATAAACAGCATTTTAGTGTTTATTTCGAGGGATAAAACCACCGCTGAGTCGCCGTTAAGATGAAGCGAAAATACGAAAATTGCGTGCTGCAGCAGAGGCAGCAACAAAAACGCTATTCGTTGTAGATATATACGTACATATATATATTTATATATACGTACATATGTGCACATATATATATGCAAATCTATACATAATAAAGAAGTGTCCGGCGGAGAATTGTATGTGCGATAATAAATAATACGAATAATAATAATTAATTCAACGGACGAAGGCGCGCGAGCGAAAGAAAAGAGGCGAGAGAAAAAGAGGCGCGAAAAGTTCGCAACAAAATGGTTGTCTCCAAAGACAACAACAAGCGTCGCCGCAAGGAGACAGAGACGCCCCCACCGCCTCCGCCCGTACCGCCCGCTGGCTCCTCGCTGGCACCTCCGTCGGCAGTGGCGGCCATCGAGTCCTCGCCCGAAATGCCCAAGAGGTGAGTCCGCCAGTCCACATATGCTCAACGATATGGGTAATGTTTAGAGGGCTTATCCATTATTACATACGTGTGTGGCACTATATGGAGATGACGTCGCAGCGTTTGTTAGCTGCTTGATTGGAATGGAAACGGATAGGGGTTGGGAGTGGGGCACTGCAGGGGGGGGGGGTCACAAGGGGGAGTAACTACGGAGGGCAGCTCTCGGTTTGTTGTATAAGCCAGCGTCAACAGCGTGTCGTGGCGTTGTTG GGGTGTTTCGCATCCGTCGTGCGCGGGGGGGGGGTCTGCTGGGGGAGCGCTCCTTCCCAGCAGGTGGATATATATATATACAGTGGTTAGTCACTAAGCTGAATATTCGGCTTTGATAATTAGGCAATATTTACCTACTCAGATTGATTTACTACGTCGTGGGTACTTCACCGAATACATAGCAAATGAATATAAAAATGATTTCTTGCATACATTTTTAATAATTGATAAGTGAAATTTATTTTACTAATTTTTAACATTACAATTGTTATTTTAGATTGTCAATTAAATCAATTGATTTACATGCAATCTATTTTCTATATTCCATACATAGTTTCAATGTTGTTTACAAATACAAAGTAATGACCTCATTGTTGTTCCTATATTCTTTCATTTATTTATATGCTAAAAATAAATTGATAGCCAAAGAATAATACACACAGAAATCCTAAAATAAAGTTGGATGGTAAATTTAGTTTTATTAACCGAATTAAACTTTTTATCGAATGTCCACTGTTTGTGATGTTGCCTTTATTATGATCCCAAATGAATATTGAATTTTCTAATTTATATCATATTGCCTTGTTACCACCAATTAAATATCTCTCACACTGCTCTTTGGCTCCTTCCATTTGCAGAACGAAGGTGCAGGCACAGCGCAAGTTTGCCCAGGGACAGAGTGCGCCCAGCAGCTCCTCCAGCATCGCATACAGCGCAGCAGCAGCGGCAGCTGGATCCGGATCGGGGCCCAGCACCTCCGGAGCGGATAGCAATCAGCCGCCTATCGAGATCCTGCCCAACAAGTGTCCAAAGCCACAGGACTTTCTTACCTTTCTATGCTTTCGTGGCACTCGCGCGTTGCCCGCCCACTTGGATTTCCTTAACCAGGGCAAGTCCAAGGATTCGGAAGCCTCGTCGACGGGAGCCAACAATAATAACAATGCTAGAAAGCCCAACAACAGTCGAGCGAGCTTGGCTGGCAACAAAAAGAAGCGCGGACGACCGGCCAAAGGCGCGACCAAGAAGGTGGATGTGGTAGCCCCTATACCCGATCCTACTGAGAAACCCTCAACAGCTGAAAAACCAGCTAGTGATAAAAACAATCTCATTGCAGCTGAAGCGCCTAAGATTCCCTCGCGAAATCCTACTCC
 

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