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CRIMIC CR92260

Gene EndoB

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:19307447..19310665	56C10	FBti0213509	91489

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACGTGTGCAATGCGACCTGCTGTAT	TCACAAGAATGCATCGACACCGAG

Images

Construct Design Sequences

sgRNA sequence	GTGGTGAACTTTCGGTGGTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGGTGCCTACGTGGAAGTC	ACCCCAAACCAATCTCAAGC	ACCGAAAGTTCACCACCACC	AAAAGCACACTCGATTTTGCA

 

Amplicon 5'

Amplicon 3'

TTGGTGCCTACGTGGAAGTCACAGTCATTCTCTATCGATCGCCTACTACTTACTTGGCCAGTTCACGCTGCAGCTGCTCCATGACATCGCCGCACTGCTTGTAGTAGCGCACTTGCGTCTGGATGAAGGCGTGCAAGTGGCGCAAATGAGATGCCTGCGATGTGCTGATCCCGTCCAGCAGCAGCTTGGTAATCTCCGCCTGCCGGTCGAACTCGGCCTGAGCCACCCGCAAGTCCCGTTCCGCCTAGAAAAAATCGCAATTAGAAATTGGCTCATTGATTAGCCATAAATCTAGAGCACGAGCTCGGTCAAGGTTTTGATACCTGTTCCAAGACGGCCTCTGGCGAGATGCCATCTTTCTAAAAATTCCGGGCAGCAGAGGGCAGAAAAAGACCGTGAGTGAAAAAGCCATTACGAAACCCAATCAATCATACCCCCTTGCAATAAGGAAACCCCATCAAATCCCACCCACTTACCGATTGCTGACCCAGCATGCTGCGCGCTTTCTTCACCCTGTTCTTGCAGGCGTCCAGATCCAGGCGCTTGGTCTCCAGGATGCCTCGCTCCTTGCCAATTGTCTTCATTTCGCCGTCTAGGAACTTGCGCAACGGCTGTGTAAAGCAAATGCCCGAGGTGGCTATAAAGTCGTGCTCGCACTGGCCCAGTTTCTGTTCCGCTTGGCCGACCTTGATCAGAGCCTGACCATAGGGTAAATCCTGCCCGAAATCGCCCCCAGCCTCGATCATGTCCAGAGCCAGGTGCTCTAGGTTGCTCAACCTTTTTGGCTTCGACTTTTCAATCTTCTCGAAGATGAAGTCTTCGACCCGGTTCTGAGGATTGGGTATCAGCACCGACTCTGTGTCGCGCACTATCTTCTCCGTCCATGTCTTGGTCACATCAGCCCGCTCGGCGAGATTCTGGAAGTGCAAGTCGTACTCGGTGCGCTCCGTGGTGCCCAGTTTCTCCTCCGTGAGCTGAAAAGGTCATTGAAAACAATGGAGAGCCGTTGAAAAGGGCAAAGAAACAACGAAAGTGCTTGAGATTGGTTTGGGGT

ACCGAAAGTTCACCACCACCCGCTTTTTTTTCAGCCACCCAAATTCATTGATTTGTTCACCTGCACCACTCGTGATATTGTGCTGCCCGCCTCCTTCACCAGGTTCTTCACATTGAAATTCGGCAGATTTATATTCATTTTTGCCGAGAGGTTCGCTAATTAACACTGCCGTTGATAAAGTTTAAATTTAAATTCCGCTACACAGCCCAATTTGTTTGTATTTATTTCCTCTACTTCGTAGTGTGACCCATTGTTTCTCCCAGGAATATACCAAATATACCGAAGAATTCCAACTGGAACAGAGTTCTAATTGGAAATACTACTATTGAGTAAAATGTAAAATTGTATTAATTTTTAATTTATCATAGCTTTTTCTTCTTTATTCTTCATGTAAATGGTGAAACGAAATTTACTTCTATTAAACAAATGCCACGACATCAAAGTAGATAACAATTAAGCTGTCTGTTAGCAAATACATACCTACATACTATGTAAGCTAACAGTTTCGGTAAATCAAGTGTGGTGAGTATCTATATAGTAACGAAAGCTACTATGGCAAGCTTTAAAAAATGTGAACGTTTGTACAATATAACATAGTCAATTAATCATGATATGGATGAAAATCATTTTTCAACATTTAAACTAATTTTTACTTACCCTGCTTGCAGACCGTGCACCTTGTAATTAAACCCCCAAAATAAAACACGATTTGGAAAACATTTAAATTTAACTTTTCACATTTAATACACACTCATTAGACATATATTATCGGAGCGGAAAAATTAAAACATGCATTCAATTGATAACTTTTCACCACTCTGCTGCTTCTTACATTTATGGCTGACTTTCGCTTTTCCTTATACATCATATGACAATCTGTGCAAAATCGAGTGTGCTTTT

 

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