CR92260

CRIMIC CR92260

Gene EndoB

Information

Donor Location Cytology FBti Stock number
pM37-p02R:19307447..1931066556C10Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AACGTGTGCAATGCGACCTGCTGTAT TCACAAGAATGCATCGACACCGAG

Images

Construct Design Sequences

sgRNA sequence GTGGTGAACTTTCGGTGGTTGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTGGTGCCTACGTGGAAGTC ACCCCAAACCAATCTCAAGC ACCGAAAGTTCACCACCACC AAAAGCACACTCGATTTTGCA
 
Amplicon 5' Amplicon 3'
TTGGTGCCTACGTGGAAGTCACAGTCATTCTCTATCGATCGCCTACTACTTACTTGGCCAGTTCACGCTGCAGCTGCTCCATGACATCGCCGCACTGCTTGTAGTAGCGCACTTGCGTCTGGATGAAGGCGTGCAAGTGGCGCAAATGAGATGCCTGCGATGTGCTGATCCCGTCCAGCAGCAGCTTGGTAATCTCCGCCTGCCGGTCGAACTCGGCCTGAGCCACCCGCAAGTCCCGTTCCGCCTAGAAAAAATCGCAATTAGAAATTGGCTCATTGATTAGCCATAAATCTAGAGCACGAGCTCGGTCAAGGTTTTGATACCTGTTCCAAGACGGCCTCTGGCGAGATGCCATCTTTCTAAAAATTCCGGGCAGCAGAGGGCAGAAAAAGACCGTGAGTGAAAAAGCCATTACGAAACCCAATCAATCATACCCCCTTGCAATAAGGAAACCCCATCAAATCCCACCCACTTACCGATTGCTGACCCAGCATGCTGCGCGCTTTCTTCACCCTGTTCTTGCAGGCGTCCAGATCCAGGCGCTTGGTCTCCAGGATGCCTCGCTCCTTGCCAATTGTCTTCATTTCGCCGTCTAGGAACTTGCGCAACGGCTGTGTAAAGCAAATGCCCGAGGTGGCTATAAAGTCGTGCTCGCACTGGCCCAGTTTCTGTTCCGCTTGGCCGACCTTGATCAGAGCCTGACCATAGGGTAAATCCTGCCCGAAATCGCCCCCAGCCTCGATCATGTCCAGAGCCAGGTGCTCTAGGTTGCTCAACCTTTTTGGCTTCGACTTTTCAATCTTCTCGAAGATGAAGTCTTCGACCCGGTTCTGAGGATTGGGTATCAGCACCGACTCTGTGTCGCGCACTATCTTCTCCGTCCATGTCTTGGTCACATCAGCCCGCTCGGCGAGATTCTGGAAGTGCAAGTCGTACTCGGTGCGCTCCGTGGTGCCCAGTTTCTCCTCCGTGAGCTGAAAAGGTCATTGAAAACAATGGAGAGCCGTTGAAAAGGGCAAAGAAACAACGAAAGTGCTTGAGATTGGTTTGGGGT ACCGAAAGTTCACCACCACCCGCTTTTTTTTCAGCCACCCAAATTCATTGATTTGTTCACCTGCACCACTCGTGATATTGTGCTGCCCGCCTCCTTCACCAGGTTCTTCACATTGAAATTCGGCAGATTTATATTCATTTTTGCCGAGAGGTTCGCTAATTAACACTGCCGTTGATAAAGTTTAAATTTAAATTCCGCTACACAGCCCAATTTGTTTGTATTTATTTCCTCTACTTCGTAGTGTGACCCATTGTTTCTCCCAGGAATATACCAAATATACCGAAGAATTCCAACTGGAACAGAGTTCTAATTGGAAATACTACTATTGAGTAAAATGTAAAATTGTATTAATTTTTAATTTATCATAGCTTTTTCTTCTTTATTCTTCATGTAAATGGTGAAACGAAATTTACTTCTATTAAACAAATGCCACGACATCAAAGTAGATAACAATTAAGCTGTCTGTTAGCAAATACATACCTACATACTATGTAAGCTAACAGTTTCGGTAAATCAAGTGTGGTGAGTATCTATATAGTAACGAAAGCTACTATGGCAAGCTTTAAAAAATGTGAACGTTTGTACAATATAACATAGTCAATTAATCATGATATGGATGAAAATCATTTTTCAACATTTAAACTAATTTTTACTTACCCTGCTTGCAGACCGTGCACCTTGTAATTAAACCCCCAAAATAAAACACGATTTGGAAAACATTTAAATTTAACTTTTCACATTTAATACACACTCATTAGACATATATTATCGGAGCGGAAAAATTAAAACATGCATTCAATTGATAACTTTTCACCACTCTGCTGCTTCTTACATTTATGGCTGACTTTCGCTTTTCCTTATACATCATATGACAATCTGTGCAAAATCGAGTGTGCTTTT
 

Search Again