CR92258

CRIMIC CR92258

Gene CG3520

CG3520

Information

Donor Location Cytology FBti Stock number
pM37-p22R:23396770..2340230959D10Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK TCAGCACCATGGATGACTTTGCCA CCTCGTAGATCTTGAACCAGCAATCC

Images

Construct Design Sequences

sgRNA sequence AATCCCCGCAATGATTAAGATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GTCGGCAAGAAGAACCAGGA CGGGGATTGTAAACGCCTCA AACGACTGTCGATTAGCGCT ACTTGGCACGTCATGTAGCA
 
Amplicon 5' Amplicon 3'
GTCGGCAAGAAGAACCAGGAGCACATGGTAGTGGTCCTTCGTTTGCTCCCGCGTGGCATGGAGCACTCGGAGCAGGCACTGCAGGCTTGGGCGCGGATCGATGCCATAGTTGGCCAGCTGCTTGATGAGACACGCTTGATAGATGCCCAAATCGATGGACTGTGCGTGGTCCTCTCGCTGCAAAAAGTGGTCGATGGCCTCAATGACCAAAATGCTGGTGCACAGACAGGTGTTCGCGTTGTTGAACTTGCTCCACGCCAACAGCTCCTGCATCAGAGCACGCGCCTCCGGTTGCTTTCCACTCAGATTGAGCAGACAGGTCCAAATGGGCTTCACATCGTGCAGAGTTTGGGGATTGCAAAGCACGTAGAGAAAGACCGGTCTCAGGTACTCTATGCTGTAGTCCCGAATGCTGAGAATGTAGTTAAGATATTTGATTACGGCTTCAACTGTCCAGTATCTCATTTAGTCTCACCTTTTGTCGTAGTGCTGACAAATGCCAATGATCTTGTTCGCCACGTCGTGCATGTTGGCACTAGAGTGCTGGAGCAGCGAGATTAGCGGATGCTGCTGCGTCTTCAGGCCATACGTACACTGATAGGACTCCTGACCCTTGAGCGACGACGTCCTGCGCTTTAGGCTTAAGAGCTGCATACCCAAGATGCTCTCCGTGATGATGGCAAAGTGCATTGGACTGGAAGCAAAGGGATTATTAAGTTCTTGTCTAAGTTAGCCACTTCAAATAGGAGTGACACTTTGAGGCGTTTACAATCCCCG AACGACTGTCGATTAGCGCTCACTTCCGGAGTAGCAGCTGCAAATCAGCACTCAAGGACGGAAGCCTCTTTTGCGGCGGCGGAGGTGGAGTGAATCGCTTTGCGTTTGACGAGGTAATTACAATTCACGCTCTGGCCACAAGTCGCCCGGGTTCGGCCTCTAATTGCAGGCCAATTAATATGCAATCCTTTTTGCCTGCACTTGCAACTCGACCGGACTGGCGGCTTATCAAGGTTAATTCCGGTATTACATGACCGCCTGTGCGTCCTTCGCCGCTCCGCAAACATTTCAGGTTCAATGCCGCAGACTTTTAGTGGCTCTGCATACATCAAATATTTGCCAGTCAAGGCTGCAGGATATGATAGGTGGGTGTAGCGCTGCGTGGCGGGAAATGGCTAATAGTTTTTACGGAGCCCCAGGCATAATCGCTGTTGATCCACGAGATTGACGACTGCGGATTGCGGCCAAGCGGAAACCGAGTCAATGCCAAACAACTGGGATTTTGGATCAGCTCCGGGTCATAATTCATTTGCGCATTTGCTACATGACGTGCCAAGT
 

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