CR92257

CRIMIC CR92257

Gene MICU1

Information

Donor Location Cytology FBti Stock number
pM37-p02L:7182234..718552927E4Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TCTAAGCAATCAGCCGACACTTCG CTTGAGTGAGATTGTGTTCGGGTG

Images

Construct Design Sequences

sgRNA sequence TGTTTAATGTGTTTGATGGTGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTGATGCAAACTAGGGGCCA GTGTTTGATGGTGGGTCAAAA ACACACACCGCGAAGATCAT TCCCTGTATTGGCGTGATCG
 
Amplicon 5' Amplicon 3'
CTGATGCAAACTAGGGGCCATAAACGTTTTGGTCATCAGGAGGAGAAGACGCCCAGTGTGACAAAGTACTTCCACATGTTCATTCTCAGTTTATTCCTCATTTCGGTAATGGACTGGGGCAAGTGAGTAAACTAACCAATAAAGTATTTTCAACCAAGTCCAAACTACTGAATTGGAATCTATTTTTAGAGTGAAACGCATGTTGACGCCAAAAGTGGACGCAGATGCTGGACAACGTCCGTCATCGGCGGCCGACGTTAATGGCGAGGATAAGTCATCCGAATCCGAATCGGAAGATAGCGAGGACGAGGAGGCAGGATCCGATCTGCACCTGCACGAGGGCAAGAAGATCCGCGAGAAAGTCGGTTTCCGCGAGAGGAAGGTATAAAGCTGCTTGACGATGCTATAACTCTGTAAAAAGTATCATTATATTCTGCTTAAATTCTGGGTCTCACAATAGTTCCAATTTTTGGTTATGTTACTAATTGTGAGGACCGGAAAAATCGTAACTTTTCTTTTAATATATTTTAAACTTTCGTATATTATGTTAAACCAAAATGTTTGATTTTTACTTTTTCTTTTATGTTTGTGACGTCTTGTTTTTGACCCACCATCAAACAC ACACACACCGCGAAGATCATTGAGTATGAGAACCGAATCAGGCAGTTCTCCACCCCCGACAAGATTTTTCGTTATTTTGCCACCGTAAGACTGCAGGATGCGACCCAAACGATTGTCTGCATGACTCCCGAGGATTTTCTTCGCTCCATTTATCCGGGAATCAAGCAGCCAGATGGTAAATCACTCATTCAAATGAGCATATGTAATTATATCTTTAGTTATTGGATTAACTTGAGTAAGGATATTTTTTTTTATTGTATTAGTTGTTTTGGTTGGCATAACTTACTTTTCATAATTTGAATAATGCTCATAATTGATGCATTTTCGTAAGCTAAACTCACGTTGTTTGCCTTAGATAATTGAGTATGAGAACCGCATACGGCAGTTCTCCACGCCGGACAAAGTGTTTCGCTACTTTGCCACCATACAGGTGCCCGTGGCGGATGATCGTCATGAGGTCTACATGACTCCGACCGACTTTCTAACAAGCATGACGCCCGGCATGAAGCAGCCAGATGGTATGTGCAAGAAAAAACCCAGGGAAAAACTTAAGAAAGCCAGTAGTTGTAGCACTTACTAACTGACATTATGATTAGAGAGCTCCTGCAATAATAGCTATATATATTTTAGGATTGGGCCTGGACCAATATCGTCGCTATGATCCCAAGGTGAGTCTAAAATGCATTTGTAATACAAGTTAATAATCCCTATTTTTCCCTACTAGTCTGTTGGCGAGCAGCTCAATTTGCATCTGGAGAAGAATAGCATTTTTTACAAACTAGGCTCTTACGGACTGATCACATTCTCAGACTACATCTTCCTGCTCACAGTCCTCTCGAGTAAGTGATAAGTATTCCCATTAGGATACATTCCAATAACCACGTTTTCTTTATAGTTTCACGACGCCACTTCGAGATTGCCTTCCGCATGTTTGACTTGAATGGGGATGGTGACGTGGACTGCGAGGAATTCGAGATGGTGGCCACCTTGGTGAGGCAGCAAACCAGCATGGGAACCCGACACCGCGATCACGCCAATACAGGGA
 

Search Again