CR92257

CRIMIC CR92257

Gene MICU1

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:7182234..7185529	27E4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTAAGCAATCAGCCGACACTTCG	CTTGAGTGAGATTGTGTTCGGGTG

Images

Construct Design Sequences

sgRNA sequence

TGTTTAATGTGTTTGATGGTGGG

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGATGCAAACTAGGGGCCA	GTGTTTGATGGTGGGTCAAAA	ACACACACCGCGAAGATCAT	TCCCTGTATTGGCGTGATCG

Amplicon 5'	Amplicon 3'
CTGATGCAAACTAGGGGCCATAAACGTTTTGGTCATCAGGAGGAGAAGACGCCCAGTGTGACAAAGTACTTCCACATGTTCATTCTCAGTTTATTCCTCATTTCGGTAATGGACTGGGGCAAGTGAGTAAACTAACCAATAAAGTATTTTCAACCAAGTCCAAACTACTGAATTGGAATCTATTTTTAGAGTGAAACGCATGTTGACGCCAAAAGTGGACGCAGATGCTGGACAACGTCCGTCATCGGCGGCCGACGTTAATGGCGAGGATAAGTCATCCGAATCCGAATCGGAAGATAGCGAGGACGAGGAGGCAGGATCCGATCTGCACCTGCACGAGGGCAAGAAGATCCGCGAGAAAGTCGGTTTCCGCGAGAGGAAGGTATAAAGCTGCTTGACGATGCTATAACTCTGTAAAAAGTATCATTATATTCTGCTTAAATTCTGGGTCTCACAATAGTTCCAATTTTTGGTTATGTTACTAATTGTGAGGACCGGAAAAATCGTAACTTTTCTTTTAATATATTTTAAACTTTCGTATATTATGTTAAACCAAAATGTTTGATTTTTACTTTTTCTTTTATGTTTGTGACGTCTTGTTTTTGACCCACCATCAAACAC	ACACACACCGCGAAGATCATTGAGTATGAGAACCGAATCAGGCAGTTCTCCACCCCCGACAAGATTTTTCGTTATTTTGCCACCGTAAGACTGCAGGATGCGACCCAAACGATTGTCTGCATGACTCCCGAGGATTTTCTTCGCTCCATTTATCCGGGAATCAAGCAGCCAGATGGTAAATCACTCATTCAAATGAGCATATGTAATTATATCTTTAGTTATTGGATTAACTTGAGTAAGGATATTTTTTTTTATTGTATTAGTTGTTTTGGTTGGCATAACTTACTTTTCATAATTTGAATAATGCTCATAATTGATGCATTTTCGTAAGCTAAACTCACGTTGTTTGCCTTAGATAATTGAGTATGAGAACCGCATACGGCAGTTCTCCACGCCGGACAAAGTGTTTCGCTACTTTGCCACCATACAGGTGCCCGTGGCGGATGATCGTCATGAGGTCTACATGACTCCGACCGACTTTCTAACAAGCATGACGCCCGGCATGAAGCAGCCAGATGGTATGTGCAAGAAAAAACCCAGGGAAAAACTTAAGAAAGCCAGTAGTTGTAGCACTTACTAACTGACATTATGATTAGAGAGCTCCTGCAATAATAGCTATATATATTTTAGGATTGGGCCTGGACCAATATCGTCGCTATGATCCCAAGGTGAGTCTAAAATGCATTTGTAATACAAGTTAATAATCCCTATTTTTCCCTACTAGTCTGTTGGCGAGCAGCTCAATTTGCATCTGGAGAAGAATAGCATTTTTTACAAACTAGGCTCTTACGGACTGATCACATTCTCAGACTACATCTTCCTGCTCACAGTCCTCTCGAGTAAGTGATAAGTATTCCCATTAGGATACATTCCAATAACCACGTTTTCTTTATAGTTTCACGACGCCACTTCGAGATTGCCTTCCGCATGTTTGACTTGAATGGGGATGGTGACGTGGACTGCGAGGAATTCGAGATGGTGGCCACCTTGGTGAGGCAGCAAACCAGCATGGGAACCCGACACCGCGATCACGCCAATACAGGGA

Amplicon 5'

Amplicon 3'

CTGATGCAAACTAGGGGCCATAAACGTTTTGGTCATCAGGAGGAGAAGACGCCCAGTGTGACAAAGTACTTCCACATGTTCATTCTCAGTTTATTCCTCATTTCGGTAATGGACTGGGGCAAGTGAGTAAACTAACCAATAAAGTATTTTCAACCAAGTCCAAACTACTGAATTGGAATCTATTTTTAGAGTGAAACGCATGTTGACGCCAAAAGTGGACGCAGATGCTGGACAACGTCCGTCATCGGCGGCCGACGTTAATGGCGAGGATAAGTCATCCGAATCCGAATCGGAAGATAGCGAGGACGAGGAGGCAGGATCCGATCTGCACCTGCACGAGGGCAAGAAGATCCGCGAGAAAGTCGGTTTCCGCGAGAGGAAGGTATAAAGCTGCTTGACGATGCTATAACTCTGTAAAAAGTATCATTATATTCTGCTTAAATTCTGGGTCTCACAATAGTTCCAATTTTTGGTTATGTTACTAATTGTGAGGACCGGAAAAATCGTAACTTTTCTTTTAATATATTTTAAACTTTCGTATATTATGTTAAACCAAAATGTTTGATTTTTACTTTTTCTTTTATGTTTGTGACGTCTTGTTTTTGACCCACCATCAAACAC

ACACACACCGCGAAGATCATTGAGTATGAGAACCGAATCAGGCAGTTCTCCACCCCCGACAAGATTTTTCGTTATTTTGCCACCGTAAGACTGCAGGATGCGACCCAAACGATTGTCTGCATGACTCCCGAGGATTTTCTTCGCTCCATTTATCCGGGAATCAAGCAGCCAGATGGTAAATCACTCATTCAAATGAGCATATGTAATTATATCTTTAGTTATTGGATTAACTTGAGTAAGGATATTTTTTTTTATTGTATTAGTTGTTTTGGTTGGCATAACTTACTTTTCATAATTTGAATAATGCTCATAATTGATGCATTTTCGTAAGCTAAACTCACGTTGTTTGCCTTAGATAATTGAGTATGAGAACCGCATACGGCAGTTCTCCACGCCGGACAAAGTGTTTCGCTACTTTGCCACCATACAGGTGCCCGTGGCGGATGATCGTCATGAGGTCTACATGACTCCGACCGACTTTCTAACAAGCATGACGCCCGGCATGAAGCAGCCAGATGGTATGTGCAAGAAAAAACCCAGGGAAAAACTTAAGAAAGCCAGTAGTTGTAGCACTTACTAACTGACATTATGATTAGAGAGCTCCTGCAATAATAGCTATATATATTTTAGGATTGGGCCTGGACCAATATCGTCGCTATGATCCCAAGGTGAGTCTAAAATGCATTTGTAATACAAGTTAATAATCCCTATTTTTCCCTACTAGTCTGTTGGCGAGCAGCTCAATTTGCATCTGGAGAAGAATAGCATTTTTTACAAACTAGGCTCTTACGGACTGATCACATTCTCAGACTACATCTTCCTGCTCACAGTCCTCTCGAGTAAGTGATAAGTATTCCCATTAGGATACATTCCAATAACCACGTTTTCTTTATAGTTTCACGACGCCACTTCGAGATTGCCTTCCGCATGTTTGACTTGAATGGGGATGGTGACGTGGACTGCGAGGAATTCGAGATGGTGGCCACCTTGGTGAGGCAGCAAACCAGCATGGGAACCCGACACCGCGATCACGCCAATACAGGGA

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