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CRIMIC CR92254

Gene Atg18a

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:8186857..8191118	66B11		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAAGCTCAAGGTGTGCCACTTTA	GAGGAAGAATCATCGCTGACAACG

Images

Construct Design Sequences

sgRNA sequence

GTTGTTGTTGTTGAGGTGGTTGG

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGCTATCTGGCCTACCCA	GGTACGTGTTTGGTGGATTCG	CACAACTACTCAACCACTCACC	TTGTCGACACCACCGGTAAC

 

Amplicon 5'

Amplicon 3'

ACTGCTATCTGGCCTACCCAGGCAGCGTGACTGCTGGCGAGGTACAGATCTTTGATGCCATCAACTTGCACGCCAAGACCATGATTCCCGCCCACGACACACCTCTGGCGGCATTGGCCTTTAGTCCCTCGGGCACGGAGATAGCCACGGCCAGTGAGCGTGGCACGGTGATCCGAGTCTTCAGCTCCCAGGATGGCAGTCGACTCTTTGAACTGCGACGGGGACTAAAGCGCTGTGTGTCCATCGTTTCGCTGTCGTTTAGCACGTGCGCCGAATATCTGGTGTCCAGCTCCAACACCGAGACGGTTCACATCTTCCGGCTGGATCGCAGTGCCACGGAAACAGCCGAAGGCCATGGCAGCAAGCAGTCGAGCGACGACTGGATGGGGTAAGCGGTGTTGGATTCGGATAAATCAACTTATGTTTTGTAGCTCTTGTAGATTGTTGTACTTTCTGCTCGATGCAGTTATGGCTTATGGTCAAATATTAAGGTGTGGGTGTTCTGTACATAATCGCTATTACCTCCCGACTCCTATGTTTAAAAACAAAACCATCTGCTATCATCCACGGCTTTCGTCGAATTATTAATATTAAACGTCACATATGTTCAGTTCCATGAACCTTTTCCCATATGGTTTAGTAATATAGAAATCGTTCTGGATCCATTTCAACTATTATTTTCATTGACCACACTGATCCATTCATATGTACATATGTAATCCTCCCATTTTTAACTTATTTGAAAGAATTTGTATTTCTACTAAAAGCCTTGTGTTTAAAATGAAATCGTTCAAGGGTTTCTCAACTAAATACTATCTGTAAATTATAATCAAGCAAGAGAACGAAAATGCCAATACAGAAAAGCCGCCATAATTTTGAACAGCGATTTTGAAAACAGGCCCAGATCAAAGTTTTTTTTTTGTACAGGACTTAAGATTTTTTGATTTTTCTCCTATTTCTCGTGTGGCACTTGACATTTATCTATTTTTCTTTTCTTTCATTTTGCGAATCCACCAAACACGTACC

CACAACTACTCAACCACTCACCATATCTACTCTCTCATGCAAGTTACTCGTTTTTTAGATTCTTAAGCAAAACGGTCACCAGCTACCTGCCCACGCAGGTGACCGACGTGTTTAGCCAAGGCCGTGCCTTCGCCTCGGTCACTCTGCCGGAGGCGGGAGTGCGCAGGATGTGCGCTATTGCCACGATACAAAAGCAGCTAAGGTGAGTCTAGCACGGATCAGTTTTGTTTCAGTTCAATTTGCAACGAAGAACGCACGGGAAAGTGAAAAAGCCATAAAATTCTACCTAGCTATAAAGAATTTCGAAAACTGCAATTCTATGCGAGCTTAATACCTCAGGTAGGGAATCTGAGTTCTAGATACCAATGCAGTTGTTCCGCGTAAATCATTTACACTACCTACTTTATCTGGCTTCATATGTTTGAATTTCCCTGAACTCTGTTGAAATGTAATTAATATGCTCTTTGCAAAAGTAGCCTAGTACATTTCATAAAGGTTTATAAAACAAAAATTGAATCACGTGCAAATTGAACTACAAACTGATTACCCTGCCTCTATTTCAAGTTCTAAATAACGGTTCATTCTGTTGTATTTGTAGACTTTTGATTGCCTCGCAGGATGGGTACTTGTACGTGTATTCCATTCCCACCGTCGAGGGCGCAGAGTGCCAGCTAATAAAGAGGCACGACCTGCGGCTGGAGGACCACTATGCCATGGATATCAAAGGTGCTTAACTGTTCGCTTCTCGCACACACTTCACACTTACACACATCCCAACCAAACCCACAATCGAAACAAATTCTCTAGTTGATTCACCTCAAACTCTAGGCCTTAACAGTGGCGTGAGCATTGGCGGAGCAGCTGGAGTTCCGCCGAGCAGTGCAAGTGCAGCCGGAGCAGGCACTGGTGGAAGTGGCGGTGATGGCAAGTCGGCTGTTTCTGTTACCGGTGGTGTCGACAA

 

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