CR92254

CRIMIC CR92254

Gene Atg18a

Information

Donor Location Cytology FBti Stock number
pM37-p23L:8186857..819111866B11Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GCAAGCTCAAGGTGTGCCACTTTA GAGGAAGAATCATCGCTGACAACG

Images

Construct Design Sequences

sgRNA sequence GTTGTTGTTGTTGAGGTGGTTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACTGCTATCTGGCCTACCCA GGTACGTGTTTGGTGGATTCG CACAACTACTCAACCACTCACC TTGTCGACACCACCGGTAAC
 
Amplicon 5' Amplicon 3'
ACTGCTATCTGGCCTACCCAGGCAGCGTGACTGCTGGCGAGGTACAGATCTTTGATGCCATCAACTTGCACGCCAAGACCATGATTCCCGCCCACGACACACCTCTGGCGGCATTGGCCTTTAGTCCCTCGGGCACGGAGATAGCCACGGCCAGTGAGCGTGGCACGGTGATCCGAGTCTTCAGCTCCCAGGATGGCAGTCGACTCTTTGAACTGCGACGGGGACTAAAGCGCTGTGTGTCCATCGTTTCGCTGTCGTTTAGCACGTGCGCCGAATATCTGGTGTCCAGCTCCAACACCGAGACGGTTCACATCTTCCGGCTGGATCGCAGTGCCACGGAAACAGCCGAAGGCCATGGCAGCAAGCAGTCGAGCGACGACTGGATGGGGTAAGCGGTGTTGGATTCGGATAAATCAACTTATGTTTTGTAGCTCTTGTAGATTGTTGTACTTTCTGCTCGATGCAGTTATGGCTTATGGTCAAATATTAAGGTGTGGGTGTTCTGTACATAATCGCTATTACCTCCCGACTCCTATGTTTAAAAACAAAACCATCTGCTATCATCCACGGCTTTCGTCGAATTATTAATATTAAACGTCACATATGTTCAGTTCCATGAACCTTTTCCCATATGGTTTAGTAATATAGAAATCGTTCTGGATCCATTTCAACTATTATTTTCATTGACCACACTGATCCATTCATATGTACATATGTAATCCTCCCATTTTTAACTTATTTGAAAGAATTTGTATTTCTACTAAAAGCCTTGTGTTTAAAATGAAATCGTTCAAGGGTTTCTCAACTAAATACTATCTGTAAATTATAATCAAGCAAGAGAACGAAAATGCCAATACAGAAAAGCCGCCATAATTTTGAACAGCGATTTTGAAAACAGGCCCAGATCAAAGTTTTTTTTTTGTACAGGACTTAAGATTTTTTGATTTTTCTCCTATTTCTCGTGTGGCACTTGACATTTATCTATTTTTCTTTTCTTTCATTTTGCGAATCCACCAAACACGTACC CACAACTACTCAACCACTCACCATATCTACTCTCTCATGCAAGTTACTCGTTTTTTAGATTCTTAAGCAAAACGGTCACCAGCTACCTGCCCACGCAGGTGACCGACGTGTTTAGCCAAGGCCGTGCCTTCGCCTCGGTCACTCTGCCGGAGGCGGGAGTGCGCAGGATGTGCGCTATTGCCACGATACAAAAGCAGCTAAGGTGAGTCTAGCACGGATCAGTTTTGTTTCAGTTCAATTTGCAACGAAGAACGCACGGGAAAGTGAAAAAGCCATAAAATTCTACCTAGCTATAAAGAATTTCGAAAACTGCAATTCTATGCGAGCTTAATACCTCAGGTAGGGAATCTGAGTTCTAGATACCAATGCAGTTGTTCCGCGTAAATCATTTACACTACCTACTTTATCTGGCTTCATATGTTTGAATTTCCCTGAACTCTGTTGAAATGTAATTAATATGCTCTTTGCAAAAGTAGCCTAGTACATTTCATAAAGGTTTATAAAACAAAAATTGAATCACGTGCAAATTGAACTACAAACTGATTACCCTGCCTCTATTTCAAGTTCTAAATAACGGTTCATTCTGTTGTATTTGTAGACTTTTGATTGCCTCGCAGGATGGGTACTTGTACGTGTATTCCATTCCCACCGTCGAGGGCGCAGAGTGCCAGCTAATAAAGAGGCACGACCTGCGGCTGGAGGACCACTATGCCATGGATATCAAAGGTGCTTAACTGTTCGCTTCTCGCACACACTTCACACTTACACACATCCCAACCAAACCCACAATCGAAACAAATTCTCTAGTTGATTCACCTCAAACTCTAGGCCTTAACAGTGGCGTGAGCATTGGCGGAGCAGCTGGAGTTCCGCCGAGCAGTGCAAGTGCAGCCGGAGCAGGCACTGGTGGAAGTGGCGGTGATGGCAAGTCGGCTGTTTCTGTTACCGGTGGTGTCGACAA
 

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