CR92253

CRIMIC CR92253

Gene CG9705

CG9705

Information

Donor Location Cytology FBti Stock number
pM37-p23L:16780100..1678241973C4Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AACCTTCGCTGTCCGTTTCACCTA GAAACTCTTGTGATGCTGCTCGCT

Images

Construct Design Sequences

sgRNA sequence GCCCCTATGCAGAGCCTTTATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGGAACAACGGGGTGAAACT TCTGCATAGGGGCCAAGTTC AATAGGCGCCGAAAGTTCAC AAATCTCCCAGGGCCACAAG
 
Amplicon 5' Amplicon 3'
TGGAACAACGGGGTGAAACTATCGAGTACTACATGTAAATAAATATATTTAAATATTCATTGTTAGTGTGGACAACATATTAGTCTGAACACATTTTTTTCGTATATCAAGCATATAAAAAAAGAATCTATATAAGAGTTGAGTGTTTGTGCGGAAAATGCAATTTTTTAATTGTTTAAAATAATTATAAATGCTTTCAATTAAATTTTCACATCTATTTATTCTACCAACAAGTTGGAGTAACTTGTAATTAATCCTGTTTGTTCGTTAAATTTGATTTTCAAATTTTATTCATGTTGCTATAAACATCGATTGCCAATTAGGCTAAGCGATAGGTTTTGGGATTTCTGGAACATTCCAAAACGTTCACTCTGAGGTCTGACTTTGACGCCTCCGCCGCCATTCGTTAAAAAACTGAAATTCTTAAAAAGCGCGCATCAAGCCGGTGTAATAATTGATAAAAAAGTTTAAAAAAATCCCAGATTCCAAAGTTGATAAAAGTCATACCGCGCAGGACTGATTTAGGGGGTACAAATAACATTGGCAACCGGCGTTTCGCACAGCGGTAAAATTTGCATCAGTGTGTGGGTGTTTCGCAAAAAAAAAAGAAGCTAAGCCAGGATTTTTGGATTGCCAATTATCAAGGTGCTAGGAAAGAGGGCGTGAAGTTGTCGGTAAGCTGACCGAGTACACGTGAATTAAGTGCAACGCAATTTGCTGACTGACTAACCCGCCCCCCGTGGCCTTGAACTCGACAGACTTGGCTGCCGTCCACTCACCCACAAACACACATACGCACATATATACACACGCGCAAGCCGCTTCTACTGTCTGAAGAGAGGAGAGGAAAAGAGCGAAGAAAGGGGAAGAACGCATAAATCGGCAAGTAAACGAGCAGTGTAAGAAAGCCATGACCGAACCAAGGACGCCCGAGAAGCTGCTGGCCGCCAAGCCGCCGGTTTTACACCACAACAGCCATAGTCCCAATGCCTCGCTGCAGCTGCCCAGTCCCATCATCACGAGGCGCACTCGCACGGCCTCGACGTAAGTCCTCCTGCACTGCCCTGCCCACCCAACCAGCAAAACCACCCACCCACTACTGGCCCCACACACACTTGACCCCAAGGGGAACTTGGCCCCTATGCAGA AATAGGCGCCGAAAGTTCACATTATTTCACAAGGTTCGCTCTTTTGTATGAAAACCAAATACAACCTTTTGAAATAGTAAAAGAATAGCATGGAAGCTTGTAATTTCTAAAGCAAAAGCTTTCTATAATGTTTTAAACTGCATTTGTTGAATTAGATGAGCAAACATAATGAAACAAACAGTAATTAGTAATCGAACATGGAACACTTTATTGGTTCCACATTTACATTTAATATATTTATTTTTATGTGTATATACTTATAAATATTTTTATAACCGTAAGGACCATTTTTTTTGAAAGTTTAAATTTGTAAACCCCATACTTTTTCAATTATTTCATTGTACATACATACATACATTCAGAAATCAGTCGCTGCCGAATTTATAAGCTCTCGGAACCCTTTCGAACATAATCCACGTACTTAAATTCTTCCCCCTCATATCCCCATGCGCATCCTCCATTGATTAACTTGTCTCTTCCTGCTTAATTCTTTTCCCAAACGCTGCCAAAACGCAAACACGCGCAAACTCAAAATAACGCTGAAAAAACATAGCTCCGCTCGGGCCTTGGAAAATCCGGTTGTGACCGGTATGGTGAAATCCTTCAGCCGCACCAAAGGACACGGCTTCATTACACCCAACGCCGGCGGAGAGGACGTTTTTTGCCACGTTTCCGAGTAAGTGAATGAATATTGCATGCGCGGCGACCTTTGCGCTGGCTCTGTAAGACCTACAAAAATGTAATCTTCCCACAGCATCGAAGGTGAATATGTGCCCATGCCCGGGGACGAGGTCAAATACCGTCTGTGCGCCATTCCGCCCAAGTATGAGAAGCACCAGGCCGTGCACGTGCAGATTAGCCACCTGACGCCGGAGGTGCACCACAAGTGGGAGGAGCCATTCTATGGTGGCTCCTCGCCCGCTAAGTAAAGCTGAAGCTTGTGGCCCTGGGAGATTT
 

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