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CRIMIC CR92251

Gene elg1

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:16132028..16136691	72D9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTAGCAACACGATGGTTCTGGTC	TTTCCGAGTCCGTCCAACGGTTAA

Images

Construct Design Sequences

sgRNA sequence

ATCGATGGAAAAAGGAAACCGGG

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGGAACTTCAAGAGGCCACG	TCGATTACTGCCATGACAGCT	ACCGGGGTTTTGTTATCATCCA	GTAACCAAGTTCCTCGGCGA

 

Amplicon 5'

Amplicon 3'

AGGAACTTCAAGAGGCCACGCAGTCGCATCAGATAAGGAAAGATGGTAAAGCGGGTGGAGGATCCTCACAGCAGTTGCTTCAAAAGTTGCAAAAGAGTGGATTAAAAGCAAAAGCAGCCGCAGTGGAACCTCCTTCCGAAGTACGGAAGTCTCTGATCCTAATCGAGGATGCGGATATCTTGTTTGATAACTTGGACGCTGGATTTACGGATGCCATTTATACATTGGCCGCCAGTTCCAAGAGGCCGGTCATTGTGGTTGCCACGGATCCGAATTGCGCGCACTTGCAGCGCCTAATGCAGCAGAATATAATCCATTTCCAAGCTCCGAACGCATTGAACATCTCCCGATTCTTGGCCGTTTTATCGCTAATGGAAAACTGTCCCATCGAGTTGGACGAATTGATATCCGTGTATCTATACAATCAACAGAATCTGCGCAAGACGTTGATGGAACTTCAGTTCTACATTCAGAGTGGTGGAGATTCAACTCGCGATAGGACTGGCGGTGGGATTAAATCACCAACCAAAACCTCAAATAGCCGGCTGGCCACCGTCGATGGCAGTCGAATCCATCAGAGATTCTTTGAGTTCTTTACAAGCCCGCAGAATGTCCAGTATCGAATACCCTTTCCCGTTGACTTCAGCCTCTTGCGTTTGAATCTCCCCGATCTTATGGCTAGTTCGGCGATGCTAAAGGAGCAGCAGGCAGCGGGCGGTGGAAGTAGAACCGCTGCCAAGCGGAAGTCCCGCTCACCAAAGAAGGCCTGGCTGAGCAGCGCCACTGGGCAGAAGTCCGATGGGCACAGCTCATCGCTCGCCACATTGGCATCCTTCTATGACAACATTTCGGTGGCTTCGTTGATGGACTCCGACTGCAGTGATCGCTTGCAGTGGCACCTGTCCGAAGAAATCGGTCACCTGCTGGTGGAGCAGGCTCTCCAAACTGGACTCGCGACCAAGGAGTGCCCCTACAATCTATTTGATAAGCCGGTTCAGAGGTAAGATTTTCAAAATGATGAGTTACTCTTTAACTAAGCCAATATATAAACACTTTAATATCAAAAGATCTTTATTAACAATATCTATCTGTATCTATATCTATTTTGCTTTAAACACATTTTAAGGCTTAATAATGCTAACTTAATTAGCTGTCATGGCAGTAATCGA

ACCGGGGTTTTGTTATCATCCAGCAGTCATAATAACAACTAGTCACAAGATATCTTTAGCGTTCGGAATTCTAATAGCCTTGTCATAAAAATGACCGATCTTATAACTATCTTAGGAACACACCTATTCATTAAATTAGTGCCCAGACTTTTCACTAATTAGATACCAAACTTATTTTAGTTATGCTTTAATTTTTTCATTTTTTATAACTTTCTGTAATCTTTCCAGGTTTTCAATTTGCCAGCACCTGGGCAACGGAGTTCTCCGTTCCGACTCCGCCAAATCGCTGGACTTCGAGCCCGCCCTGCGCTCCATATGCCGCAGCGAAAAGGAACGGGCAGGACTGGAACGGAAGTCGAGTAGATTCTATCACTATCTGCGCAACCACACGGTAAACGTGACCAGTTTCACGACGGATTACTTTGACACTGCATGCAGTACGTTCCAATCGGCTGCCCCCAGCAGCGAGCCAACCATGGAGGCCGAACCGAAAGATTAGGCATCGTTTGGATTTAGTTTAATTTTTTTGAAATTGGTGGAGCAGCTCCGCCGGCTTTGTTTGCAATTTATTCAGATCGTTTAAGAAGAGTTGATCTTTTTTAAGATAGCTTTGTGGACATGAAGATGTAGCTATTATGAGATAAACCTAGAACTATTTACAATTTAACTATGATAAACTAACTACGACCTCATCCTATGTTAATAAATCAATCAAATGACATGTAAACCTATGACTAGCAGCAACAGAAACCGGAGGAGCTATTCTTCGCGGGTTCGGGATCTTCGGATATTGTGTTTGCCGTCGGCTTTGCTGCACCATTGGACGTTGGCTGCACTTGAATGGGTGCAGCTGAGTTGATTTCTCCGATTTTGTAGTTCTTTAGATCCTTTTCTGCGTCGGAGGAATGGCCTGCCTGCTTGAAGGCCTTGGTGGCATCCTTGCCGCCATATTCGAGTAGTGCTTCACTGCCGCCAGGATGCTCGCCGAGGAACTTGGTTAC

 

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