CR92251

CRIMIC CR92251

Gene elg1

Information

Donor Location Cytology FBti Stock number
pM37-p23L:16132028..1613669172D9Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AGTAGCAACACGATGGTTCTGGTC TTTCCGAGTCCGTCCAACGGTTAA

Images

Construct Design Sequences

sgRNA sequence ATCGATGGAAAAAGGAAACCGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGGAACTTCAAGAGGCCACG TCGATTACTGCCATGACAGCT ACCGGGGTTTTGTTATCATCCA GTAACCAAGTTCCTCGGCGA
 
Amplicon 5' Amplicon 3'
AGGAACTTCAAGAGGCCACGCAGTCGCATCAGATAAGGAAAGATGGTAAAGCGGGTGGAGGATCCTCACAGCAGTTGCTTCAAAAGTTGCAAAAGAGTGGATTAAAAGCAAAAGCAGCCGCAGTGGAACCTCCTTCCGAAGTACGGAAGTCTCTGATCCTAATCGAGGATGCGGATATCTTGTTTGATAACTTGGACGCTGGATTTACGGATGCCATTTATACATTGGCCGCCAGTTCCAAGAGGCCGGTCATTGTGGTTGCCACGGATCCGAATTGCGCGCACTTGCAGCGCCTAATGCAGCAGAATATAATCCATTTCCAAGCTCCGAACGCATTGAACATCTCCCGATTCTTGGCCGTTTTATCGCTAATGGAAAACTGTCCCATCGAGTTGGACGAATTGATATCCGTGTATCTATACAATCAACAGAATCTGCGCAAGACGTTGATGGAACTTCAGTTCTACATTCAGAGTGGTGGAGATTCAACTCGCGATAGGACTGGCGGTGGGATTAAATCACCAACCAAAACCTCAAATAGCCGGCTGGCCACCGTCGATGGCAGTCGAATCCATCAGAGATTCTTTGAGTTCTTTACAAGCCCGCAGAATGTCCAGTATCGAATACCCTTTCCCGTTGACTTCAGCCTCTTGCGTTTGAATCTCCCCGATCTTATGGCTAGTTCGGCGATGCTAAAGGAGCAGCAGGCAGCGGGCGGTGGAAGTAGAACCGCTGCCAAGCGGAAGTCCCGCTCACCAAAGAAGGCCTGGCTGAGCAGCGCCACTGGGCAGAAGTCCGATGGGCACAGCTCATCGCTCGCCACATTGGCATCCTTCTATGACAACATTTCGGTGGCTTCGTTGATGGACTCCGACTGCAGTGATCGCTTGCAGTGGCACCTGTCCGAAGAAATCGGTCACCTGCTGGTGGAGCAGGCTCTCCAAACTGGACTCGCGACCAAGGAGTGCCCCTACAATCTATTTGATAAGCCGGTTCAGAGGTAAGATTTTCAAAATGATGAGTTACTCTTTAACTAAGCCAATATATAAACACTTTAATATCAAAAGATCTTTATTAACAATATCTATCTGTATCTATATCTATTTTGCTTTAAACACATTTTAAGGCTTAATAATGCTAACTTAATTAGCTGTCATGGCAGTAATCGA ACCGGGGTTTTGTTATCATCCAGCAGTCATAATAACAACTAGTCACAAGATATCTTTAGCGTTCGGAATTCTAATAGCCTTGTCATAAAAATGACCGATCTTATAACTATCTTAGGAACACACCTATTCATTAAATTAGTGCCCAGACTTTTCACTAATTAGATACCAAACTTATTTTAGTTATGCTTTAATTTTTTCATTTTTTATAACTTTCTGTAATCTTTCCAGGTTTTCAATTTGCCAGCACCTGGGCAACGGAGTTCTCCGTTCCGACTCCGCCAAATCGCTGGACTTCGAGCCCGCCCTGCGCTCCATATGCCGCAGCGAAAAGGAACGGGCAGGACTGGAACGGAAGTCGAGTAGATTCTATCACTATCTGCGCAACCACACGGTAAACGTGACCAGTTTCACGACGGATTACTTTGACACTGCATGCAGTACGTTCCAATCGGCTGCCCCCAGCAGCGAGCCAACCATGGAGGCCGAACCGAAAGATTAGGCATCGTTTGGATTTAGTTTAATTTTTTTGAAATTGGTGGAGCAGCTCCGCCGGCTTTGTTTGCAATTTATTCAGATCGTTTAAGAAGAGTTGATCTTTTTTAAGATAGCTTTGTGGACATGAAGATGTAGCTATTATGAGATAAACCTAGAACTATTTACAATTTAACTATGATAAACTAACTACGACCTCATCCTATGTTAATAAATCAATCAAATGACATGTAAACCTATGACTAGCAGCAACAGAAACCGGAGGAGCTATTCTTCGCGGGTTCGGGATCTTCGGATATTGTGTTTGCCGTCGGCTTTGCTGCACCATTGGACGTTGGCTGCACTTGAATGGGTGCAGCTGAGTTGATTTCTCCGATTTTGTAGTTCTTTAGATCCTTTTCTGCGTCGGAGGAATGGCCTGCCTGCTTGAAGGCCTTGGTGGCATCCTTGCCGCCATATTCGAGTAGTGCTTCACTGCCGCCAGGATGCTCGCCGAGGAACTTGGTTAC
 

Search Again