CR92246

CRIMIC CR92246

Gene CG33947

CG33947

Information

Donor Location Cytology FBti Stock number
pM37-p13L:11096496..11098556N/AOrder from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band long band ACTGAATCAGCTGTTCCTCGCTGA TGTGATGGTCTACCGACTTGTTGC

Images

Construct Design Sequences

sgRNA sequence TTAGTCATTGTCCTTGGGATCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AATACACGTTCAGCTGCCGA AGGACAATGACTAACGAGGAGC GGGATCTGGTTCAGCGAGAA CATACTGTGGCTGCCTCCAA
 
Amplicon 5' Amplicon 3'
AATACACGTTCAGCTGCCGACCAGCCAAATACAACAAAGCAACGCTGTAAAAACTGAAAACACATTTCAGTTTATTTCGCCTCGTGCCATGTGAGCAACTTCAAATTGATATTGAATCGGAATCAGTGTGCGGGTGGTGCTAATTAGCGGAAGACGGCAATTTATTGAACGCGAAAAAAGCCCCACAATCCAACTTCCATTTCGACACGAAACCAACCACCCGCCAAATCAAATCGCCGTCGAGTTGTGCATCAAATGAATGTGCGAAAAGTGCAGTAAATCAATGTTTTTGTGAGTGTTTGAAAGAAGAAGACGGAAGGAGCGCCCACAACAAAAGCAAAGAGAGCAAGACCTAACGGGACACCCGAAACCAAAAACCTATCGGCACAACGACACTTTCTCAATAGCTATAGTTTTAGTTCATATGTTCATATCTCGGAAAATGGACGAGGTCTTTAGCCTACACATGGAGAAATTGGACGTTTACGACGGTTAGTATCTAATTTGCCGGAAGTTCTACTTAAACGTAGAACATATGTATATGATGGAATCTGGGTTGTATTTCTGATTAATGAGTTCATCAACTTTCAAGGAAATAATAATAGTAGTAGTAGTTGTAAACAGCTGAAGTATTGGGTATAAAATAACACTGATATGGGTAAAATCATAATAGACACTTTATTTGATTCAAGACTCGCATGATTTTAGCTGTATGAATCATGTCGAAAATAATAGAAATCACTATTACTAAATATAGATAATTTTAAAATTTAGATTCAGTGCAACATGGATACAGTGATTAAGTGTTACAATATAAACAAAAGTAAAAGAAAAGTACAACAAAAACAAGTATTTGGTGAGAAATGATAAAAACTCACCAATAATGAAAACCATTTATGTAGGATTTTAACAAACACTGTTCTCGTCTGCATGAGTGCTTGTCTTTGTAATAGAATTCGAGCAATTGCACCATTTTAATCGAAAAACTAAGATGATACACATGGTTAACAACCATGGGAAATGGCTTAAAAGTATGAAAATATAAGAAGGTAAATCAGGAGAAGCTCCTCGTTAGTCATTGTCCT GGGATCTGGTTCAGCGAGAATCTCCTGGATGATTCGATGGCCGTCCTCCGTTTTCTCACCCTGAATGATCAGATCTCTAAGCGTAGATATATGTTTTTCACTCGTCTCGATCTGAACCTTAAGAGCATCGTTTTGCTTCCTCAAATCATCGATGCGTTTCTTACGTTCCTCGGCCGATTTCTTGGTCTTTTCGCGTGTGCGCTGAACAGCCTGTTCCGAGAATAGTAATAAATCAATAGTGGGCATCTATTTTTGGGGACTTCTGGGGGCCACGGCGCCACAGCAACATGTTTCCCAATCTTTGCTCTCTGTTTACATCCAAAACTCTTAAACTTACCTCATTGTTTTTCTTTCTTTTTTCTTTGTACGCTGGATCGTCTGTGTGCGGACTTAATGGCGAATCGCTATTTTTGCTGGTCGACGCGGCAGTTCTCTTTTTGGCCGGCATTATCTCCGTGATAATACTGATATTTATTTAGTTCAAATTGGATCGCAATCGGACGGAAATGCAGAAGAAAACACAGCGATGTATGCGTTGTTTTGTTGTGAAATTACACGATAAAACAGCTGTTCATTAGCCCTCCCAGTGTTGCAAAATGCGCTGCCTACACTGCCCAGCCAACAAACAGTTAGCGGTATTATTTAATTAAATTAATTGCATGTTGAAAATCAATATTAAAACTCACCCAAAACCATAGCCATGGAATGCTATTCAAAAACTGTCAACCAGACACTTTATTTGGTTTGCTCAGACTTTCACTTTTTAATTTCTCAACTGTTTATACATTTTATTTGTAAAGACTGATTGCTTGTACGTAGATGTAGTTTAATGCAATAAAATTAAATGAAATGTAATACGTAATAGTCAATAAATGTTTTAAATAAGCAACAGTTAAAATTACCAACGTGTTTTTTCCAACACTCCACCCAATAAACAAATTGTGTCAGAAGTATTGGCTGCCGGCGCTTTACAGTACTGGATTATTTGCAACCGGCTTATTATTGCACAAAAATCTAGTGAAATGTCCTCGCCAGCCCCCAAATCGCCAGAGCTTTCGGATAAAAGCAAGAAATACGACAGACAAATCAGGTGAGGAGCGTTTTAATTGATAAATTCGGAAGCATAGCACTGATGACACCTATTTAGGCTGTGGGGAGAGCATGGACAGACGCTATTGGAGGCAGCCACAGTATG
 

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