CR92244

CRIMIC CR92244

Gene CG5577

CG5577

Information

Donor Location Cytology FBti Stock number
pM37-p13L:17838650..1784142775A4Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TGCACTAACAACTCGACGAAGACC AGCGATAGCATGCCACAATTGCTG

Images

Construct Design Sequences

sgRNA sequence TGGGCTTGTGAGTGCTGCTATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GTGCCGGGGATACAAAAGGA CAGAGTTGTCGCTTCACTGA GAACCCTCTCTCTGTGGCAC GTGTCTGTTCGTGGCTACCA
 
Amplicon 5' Amplicon 3'
GTGCCGGGGATACAAAAGGATGGATACTTGTGCACGGCATCCCGGTTGGTGACCAAAAAGGCGGCGTCGGGATTACTGCAGAGGATGTGACAGGCGCGCGCCATCTTGCAGTAGCTAAAGTACTCATCCCAGCCGACCACCACGGCACCCACGTCCTTCGCCGCCTCTGGGTTCCCAAACTCGCGTGTCACAAAGTCCGGCCATTTGTCGCCCGGAGGCAGTTCATCTGTTCCGCCGGCTCCGTAGCTGTCGATTCCGCGCTGCCTTAATTCCCGCGCAATGGCCGCATTGCCCACCACATAGACCTTGTGCCGGGTCCTGTCGAACTTCGGACTCCCAACCAGATAGTCGGCGATGGCCGCCGTGGGCGAAATGATGTGTCGGTCGCTGGGTAAATGGAAGCCAAGGCGCTGGGAGCGCTCAAAGAGCTCCTGTCGCGTCTTTAGGCCATTGTTGGTAATCAGGTAAACTTTTTTGTCAAATCGATCCTGCAGGGCATTCACCACATCCGGAGCACCAGCGATGGCCGTGTCATCCTGCCATATGGTGCCTGTGCGTTTAATGGATATTTGATTTGGTGTCATTTGAAGAGGTTTGAAATGAGAGATATATTTTATAAAGTAACTAAATAATATTGGAATTAACACGAGTTTTGGCTTTATTTTAAAATAATTACGAACGAAATTTCTGTTTGGTAAGCCCAATAGTACATGGGAAATTTATACATTAAATTGGAATGATCTGCGAGAGATCAGAGAGATTTTCAGGTATAAACATATTCCTATTGATCAGGTATAAATATATTCCTAAAAATGTAAAATGATTTCAGCAGCGTTAAGCCTTATCAACTTTTTCTAATTATTAACGATTTTACGTTTAAAGTTATCCACTTACAAAATGAATAACAGTAACAATCGATGTAGCAGGCCGATGAAATGTAAACTTTTAGCTACCGCGATCGCCAAGCTTTTCCCCTTACCCATGTAAATTACCATCGATATTTTATACTCTGTTTTTTCAGTGAAGCGACAACTCTG GAACCCTCTCTCTGTGGCACCTTAGCCGCCCTTGAGACTCACCGTCGCCGTCGCAGAGAACCGTGTCGAAACTTTGGAGCCACTCGCTCACCTGCTCCTCGGATAGGCCGGTCAAGTCGATAGCACCGCCTCTGGACATTTTGCTTCGTAGACCGCTTCGCCAAATGCTTGCGACTCAACTAAATCGACGGCGACGGGCTGGCTGATTTCGATTCACGGTGATTGGGGAAAATCTGCTATCTCCCTTTCGCACAAAGCTCGCTCGGAAAAACAAATTGAGTCGGGCATATCGGTTAAATCATCCGTCTCACAAAATGCTTTTGAATTTATTTAAATATTCTTTGTTCTGTGGTATAAATTGCACTATAAATTTGCGTTAAGTATTAATAATCTTTCCTGTCTATTGAAGTTAAATGTAAAATCTATACAAACACATGTCCGTACTATTTACTGATTACTGATTCATTTCAAGTCCGTCAGGCTGTTCACGATCGACGGCAACAGATCTCCCAACTTGGGCAGGTACACGTCTGGTATGAGCTTCTTCTCCTCGGGATCCTGGCTCAACTTCCAACGCTCCACGTCCTTCAGCTGATGGATTCCGGAACCTACAAGCAGGGTCTGGAAGCCGCAATTGAACCCAAGAAGTATGTCCGTATTGGCTCGATCTCCGATCATCAGAGTTCTCGATGGATCGATCTTCTTCTCCGTGACCAGAGACTCACAAATGGCAGGATTCGGCTTCCCGATCACTACAGGATCCCGCTCCGCACAGGTTTGAATGGCCCGCACGAAGCTCCCGCTGCCTGGTACTATCATATTGGGCATGGGGAAACGCTCATCCGTATTGGTAGCCACGAACAGACAC
 

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