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CRIMIC CR92244

Gene CG5577

CG5577

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:17838650..17841427	75A4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCACTAACAACTCGACGAAGACC	AGCGATAGCATGCCACAATTGCTG

Images

Construct Design Sequences

sgRNA sequence

TGGGCTTGTGAGTGCTGCTATGG

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGCCGGGGATACAAAAGGA	CAGAGTTGTCGCTTCACTGA	GAACCCTCTCTCTGTGGCAC	GTGTCTGTTCGTGGCTACCA

 

Amplicon 5'

Amplicon 3'

GTGCCGGGGATACAAAAGGATGGATACTTGTGCACGGCATCCCGGTTGGTGACCAAAAAGGCGGCGTCGGGATTACTGCAGAGGATGTGACAGGCGCGCGCCATCTTGCAGTAGCTAAAGTACTCATCCCAGCCGACCACCACGGCACCCACGTCCTTCGCCGCCTCTGGGTTCCCAAACTCGCGTGTCACAAAGTCCGGCCATTTGTCGCCCGGAGGCAGTTCATCTGTTCCGCCGGCTCCGTAGCTGTCGATTCCGCGCTGCCTTAATTCCCGCGCAATGGCCGCATTGCCCACCACATAGACCTTGTGCCGGGTCCTGTCGAACTTCGGACTCCCAACCAGATAGTCGGCGATGGCCGCCGTGGGCGAAATGATGTGTCGGTCGCTGGGTAAATGGAAGCCAAGGCGCTGGGAGCGCTCAAAGAGCTCCTGTCGCGTCTTTAGGCCATTGTTGGTAATCAGGTAAACTTTTTTGTCAAATCGATCCTGCAGGGCATTCACCACATCCGGAGCACCAGCGATGGCCGTGTCATCCTGCCATATGGTGCCTGTGCGTTTAATGGATATTTGATTTGGTGTCATTTGAAGAGGTTTGAAATGAGAGATATATTTTATAAAGTAACTAAATAATATTGGAATTAACACGAGTTTTGGCTTTATTTTAAAATAATTACGAACGAAATTTCTGTTTGGTAAGCCCAATAGTACATGGGAAATTTATACATTAAATTGGAATGATCTGCGAGAGATCAGAGAGATTTTCAGGTATAAACATATTCCTATTGATCAGGTATAAATATATTCCTAAAAATGTAAAATGATTTCAGCAGCGTTAAGCCTTATCAACTTTTTCTAATTATTAACGATTTTACGTTTAAAGTTATCCACTTACAAAATGAATAACAGTAACAATCGATGTAGCAGGCCGATGAAATGTAAACTTTTAGCTACCGCGATCGCCAAGCTTTTCCCCTTACCCATGTAAATTACCATCGATATTTTATACTCTGTTTTTTCAGTGAAGCGACAACTCTG

GAACCCTCTCTCTGTGGCACCTTAGCCGCCCTTGAGACTCACCGTCGCCGTCGCAGAGAACCGTGTCGAAACTTTGGAGCCACTCGCTCACCTGCTCCTCGGATAGGCCGGTCAAGTCGATAGCACCGCCTCTGGACATTTTGCTTCGTAGACCGCTTCGCCAAATGCTTGCGACTCAACTAAATCGACGGCGACGGGCTGGCTGATTTCGATTCACGGTGATTGGGGAAAATCTGCTATCTCCCTTTCGCACAAAGCTCGCTCGGAAAAACAAATTGAGTCGGGCATATCGGTTAAATCATCCGTCTCACAAAATGCTTTTGAATTTATTTAAATATTCTTTGTTCTGTGGTATAAATTGCACTATAAATTTGCGTTAAGTATTAATAATCTTTCCTGTCTATTGAAGTTAAATGTAAAATCTATACAAACACATGTCCGTACTATTTACTGATTACTGATTCATTTCAAGTCCGTCAGGCTGTTCACGATCGACGGCAACAGATCTCCCAACTTGGGCAGGTACACGTCTGGTATGAGCTTCTTCTCCTCGGGATCCTGGCTCAACTTCCAACGCTCCACGTCCTTCAGCTGATGGATTCCGGAACCTACAAGCAGGGTCTGGAAGCCGCAATTGAACCCAAGAAGTATGTCCGTATTGGCTCGATCTCCGATCATCAGAGTTCTCGATGGATCGATCTTCTTCTCCGTGACCAGAGACTCACAAATGGCAGGATTCGGCTTCCCGATCACTACAGGATCCCGCTCCGCACAGGTTTGAATGGCCCGCACGAAGCTCCCGCTGCCTGGTACTATCATATTGGGCATGGGGAAACGCTCATCCGTATTGGTAGCCACGAACAGACAC

 

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