CR92242

CRIMIC CR92242

Gene Zcchc7

Information

Donor Location Cytology FBti Stock number
pM37-p13L:16845386..1685235073D1Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK TTCCGATAGCCAGAGTGTCATCAC GGAGACTGCTGCTTTCGCTGTTTT

Images

Construct Design Sequences

sgRNA sequence GATGGCCAGCGTTAGTGTGCTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGTTGGCCCAAATACCCGTT CACTAACGCTGGCCATCAA GCTCGTGTGTGGATGGCTTT GGTGGGATGGCGACTAGAAG
 
Amplicon 5' Amplicon 3'
TGTTGGCCCAAATACCCGTTATTCCTACCGATCTAATGCATTCCAATCCGCAGCTTGAGGAGATGGACAGCGAACGGCTGAACGAGCTAGAATCAATTCTATATGCCTCGATCCACTACAGCGATGGATTCGAAGAGCACACTGCCATGGATCAGCCTGCGAAGGAGTCAACCAGCGACCAGGATGCCAGGAGCATGCCACCGCCTCCTCAGCAGAAGCATCCCATTCCCGGACAGCGTTTCGTCAGCGGAACACGAGTGATCAACAACAGCATGCAGAAACCACGCTACTGGATCGATGGTGCGGCAGCTGTGGGGGAGGGGCAGGTGGTGCAGGCGGAGGAACAGCAGGCCACCACAAACAAAACAATGCCAGGTGAGTGGCAACCATTGGCAAATGGTATATATGAAGTAATAAAAACCATAAGCTAGCATAAAAATATACCCAAAAATGTACCCAAAAAAGGTAGGATCCAATAAGATCCAATATCTCACAAGTTATTAGGTAATTTAAATCATAGTAAATATAATCATATTTAATCGGTCGATTTGTAGCTATAACTGTTTAAAACTCAAAACAAAAAAAATTAAACGTGATTCATAATCTTAGGTATTCTTAGCTAGTTCATTTGTAGTCTTTTAGTTCCGCCCCATTTCATAGTAGTCAAGAACATTTCCTACTTCCATCAGTGGGGATCAGCCCAGCTTTTGGGCATTAGGCTGCTTTCATCCTTTTCAAAACTATTTCGTTGGCGTGCTTTCAAAATTCTCCATAACCTTCCAATGATGACCTGCTTCCTCAGTCGCTTTCTGGGCCTGGGCAGGTGCGGTCTGCAGGTGCTCGAAAGGCGGATGGGCCATTTCAACCATTGCACCTTGGCCCTGGCCACTCTCGACCTTGTCTGCATTGTTTGCCTGCTCCATTACCAACTGGCGCGGCATGGCCGAGATCTGTTTTTTTGGTGCGAGGAACTGGACCAACGACTAGTGGAGTACATGCTATCTGCAGTCGTATTGATGGCCAGCGTTAGTG GCTCGTGTGTGGATGGCTTTATCTTCTCTGCCTTGGTTCAGCGGAAAATGAGTCGGTTTGATATGCCGCGACAGTATTTTGAAGATCGTTACCGCAGATTCGTCTTCATGCGGCTGGTCAGGCAACTAGAGCAGGCTCTCAAAGTGCAGGCTAAGCAGTCTGAGTTGGGCGAAAGAATGATGACGCCACTCGCCAATCTGTTCGAGGCGCAGCAGCTGATCAAGGAGGCAATCGATGAGTTTCGCACAGCGGTGCGAGTCCTACTGTGGCCCAATCGATCCGATGTGCTGGCAGAGGCGTTTGTCCTCTTCCACATAAGCGAATCGCAGTTGTCGGATCTGACACTTCAAGGCTACAGGCTGAACGACGTCGAGGGGGAAGACATGTCCAATTCCCGTCTTAGTCAAATGCTTAACCCACTTTGGTATTAAAATTGGTGCTATAGTTCAGAAACTGCATTTTTAATTTGTCAATGAAAATGTTGGGTTGTATCAAAAGATTTGGTTTATTCTTAAGAATTTCTGCTAATTTGCTATTCACATTTATTCATATCGCCTATACAAATAAATATAAGAATCACATTTTAATTTATGGTTCTTGTCTCTTAGCTGTTGAAGAAAGTTCCTCATCACAATCGATGGATGTCGATATGCCGCAAGACAAGAGTCTATTGCTGTCGCCCGAAAAGGGAACGTCCAATCAACCGAAAACTGCTAGCCAGCCCCCGAAACATCAGCAAATCCCACAGGCCAAGCAACAGCAAAATATTCATGCAAAACAATTGCAAAATCAACAGGCAAAGCCTCAGGAGCAAAAAAATTCACAACAGAAACAGCAGCAGAGTCTTAAGCAACAAAATCAACAATCCAAGCAGCACCAAAATCAGCAAAAGAAGTCGGCGCCAGTAGTACAAAAACCAAAACCGCAGCCAAACGCATCCACCAAAGTGAATCACGAAGCCTACGAGGCGGAGCGTTTTGACCAGAGACTTCTAGTCGCCATCCCACC
 

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