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CRIMIC CR92242

Gene Zcchc7

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:16845386..16852350	73D1	FBti0213502	91482

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	TTCCGATAGCCAGAGTGTCATCAC	GGAGACTGCTGCTTTCGCTGTTTT

Images

Construct Design Sequences

sgRNA sequence	GATGGCCAGCGTTAGTGTGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTTGGCCCAAATACCCGTT	CACTAACGCTGGCCATCAA	GCTCGTGTGTGGATGGCTTT	GGTGGGATGGCGACTAGAAG

 

Amplicon 5'

Amplicon 3'

TGTTGGCCCAAATACCCGTTATTCCTACCGATCTAATGCATTCCAATCCGCAGCTTGAGGAGATGGACAGCGAACGGCTGAACGAGCTAGAATCAATTCTATATGCCTCGATCCACTACAGCGATGGATTCGAAGAGCACACTGCCATGGATCAGCCTGCGAAGGAGTCAACCAGCGACCAGGATGCCAGGAGCATGCCACCGCCTCCTCAGCAGAAGCATCCCATTCCCGGACAGCGTTTCGTCAGCGGAACACGAGTGATCAACAACAGCATGCAGAAACCACGCTACTGGATCGATGGTGCGGCAGCTGTGGGGGAGGGGCAGGTGGTGCAGGCGGAGGAACAGCAGGCCACCACAAACAAAACAATGCCAGGTGAGTGGCAACCATTGGCAAATGGTATATATGAAGTAATAAAAACCATAAGCTAGCATAAAAATATACCCAAAAATGTACCCAAAAAAGGTAGGATCCAATAAGATCCAATATCTCACAAGTTATTAGGTAATTTAAATCATAGTAAATATAATCATATTTAATCGGTCGATTTGTAGCTATAACTGTTTAAAACTCAAAACAAAAAAAATTAAACGTGATTCATAATCTTAGGTATTCTTAGCTAGTTCATTTGTAGTCTTTTAGTTCCGCCCCATTTCATAGTAGTCAAGAACATTTCCTACTTCCATCAGTGGGGATCAGCCCAGCTTTTGGGCATTAGGCTGCTTTCATCCTTTTCAAAACTATTTCGTTGGCGTGCTTTCAAAATTCTCCATAACCTTCCAATGATGACCTGCTTCCTCAGTCGCTTTCTGGGCCTGGGCAGGTGCGGTCTGCAGGTGCTCGAAAGGCGGATGGGCCATTTCAACCATTGCACCTTGGCCCTGGCCACTCTCGACCTTGTCTGCATTGTTTGCCTGCTCCATTACCAACTGGCGCGGCATGGCCGAGATCTGTTTTTTTGGTGCGAGGAACTGGACCAACGACTAGTGGAGTACATGCTATCTGCAGTCGTATTGATGGCCAGCGTTAGTG

GCTCGTGTGTGGATGGCTTTATCTTCTCTGCCTTGGTTCAGCGGAAAATGAGTCGGTTTGATATGCCGCGACAGTATTTTGAAGATCGTTACCGCAGATTCGTCTTCATGCGGCTGGTCAGGCAACTAGAGCAGGCTCTCAAAGTGCAGGCTAAGCAGTCTGAGTTGGGCGAAAGAATGATGACGCCACTCGCCAATCTGTTCGAGGCGCAGCAGCTGATCAAGGAGGCAATCGATGAGTTTCGCACAGCGGTGCGAGTCCTACTGTGGCCCAATCGATCCGATGTGCTGGCAGAGGCGTTTGTCCTCTTCCACATAAGCGAATCGCAGTTGTCGGATCTGACACTTCAAGGCTACAGGCTGAACGACGTCGAGGGGGAAGACATGTCCAATTCCCGTCTTAGTCAAATGCTTAACCCACTTTGGTATTAAAATTGGTGCTATAGTTCAGAAACTGCATTTTTAATTTGTCAATGAAAATGTTGGGTTGTATCAAAAGATTTGGTTTATTCTTAAGAATTTCTGCTAATTTGCTATTCACATTTATTCATATCGCCTATACAAATAAATATAAGAATCACATTTTAATTTATGGTTCTTGTCTCTTAGCTGTTGAAGAAAGTTCCTCATCACAATCGATGGATGTCGATATGCCGCAAGACAAGAGTCTATTGCTGTCGCCCGAAAAGGGAACGTCCAATCAACCGAAAACTGCTAGCCAGCCCCCGAAACATCAGCAAATCCCACAGGCCAAGCAACAGCAAAATATTCATGCAAAACAATTGCAAAATCAACAGGCAAAGCCTCAGGAGCAAAAAAATTCACAACAGAAACAGCAGCAGAGTCTTAAGCAACAAAATCAACAATCCAAGCAGCACCAAAATCAGCAAAAGAAGTCGGCGCCAGTAGTACAAAAACCAAAACCGCAGCCAAACGCATCCACCAAAGTGAATCACGAAGCCTACGAGGCGGAGCGTTTTGACCAGAGACTTCTAGTCGCCATCCCACC

 

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