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CRIMIC CR92237

Gene CG14186

CG14186

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:20098084..20103827	76F1	FBti0213501	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTCCAAATGGAGGCAAAGGTGCA	CCATTACACCCAAACTCACCGATC

Images

Construct Design Sequences

sgRNA sequence

AAAGTGGTGGGTGGATGTGCAGG

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAAGCCGTGGCATATGTTCG	CCACCCACCACTTTCTCGAT	ATGGGTGGATGGGTGGATGG	CTCACTCACCTGCTGATCCC

 

Amplicon 5'

Amplicon 3'

CAAGCCGTGGCATATGTTCGAGATGAGCAGCCAGATTCGTTGAACTACACAGGCTAGTTGTATGTTGCTGGTGATACCATTGCTAGTAAAATTAAATAAATTGCTGGATTAGAGTGACATCACTGGCATCTACACTACACTATTGTGCGAATGTTTAAATATATTTAAATTCTCAAATAGTTAAAAACAAATAATTTAAATGAGTATTTTCAGCAGAATATTAGGAAAAGTTTTGTGATAACCTTAGATTTGGACAGTTTCATATGCATAACTGACGTCTGAGGTATCATTTCTATTTTCCGCAACTCACCGATTAAAGTCGGCATCCAGCAAACAGCGGGTATCACCGTAGCTGCCACGCCCACTTCCATTTCCGCCCATTCTGTCGCCGCCGTGGGCGTGGCTGCCGCTGGAGCGGAAGAGGCGACCGGCGCCTCCAAGCTCCGCGGCCGAGATGCCATCGAGGCGACCTCCGCCACCGCCTGCACATCCACCTCCGCCGCCGGATGCCGAAATTCCGGCGGATCCACGAACAGCCGCCCCCAGGAAGGAGCGCTTGTAGTAGCTGTGCTCCACGGGTCGATATCCGGATGACGTCTGCAGGAACATCTTGTCGATGTGCGCGGGCGCATCGTCCGTGGGCGAGACGACCGGCGTGAATGGGTTGTAGATCTCCTCGCCATCGGCCGGAGCTATGTCATCGGCCAGCTCCTTCAGAATCGAGGATATACCGCCTGCTGAGTACAACTTGGCTGTGGGAGAAAGAGGGATTCCATTCGGCTATTGTTTGTTACTTTGTTTTTCCATTCTCAGCTTCTTATGAGAACCATTTGTTATAAGTTTGTTATAAACAGAAAATGAATCAAATAAATTGTAATTGTTTGTGAAATAATCGGTGTATCAAGTTTTACTTAGTTTATTTAAAGAATAATCAAACAAGCTCGTCTAAGACGGCATGGTATCATGGTATCAAACGAGCCAGTGCGCAGAATCGAGAAAGTGGTGGGTGG

ATGGGTGGATGGGTGGATGGGTGGATGGGTGGAGCTTTTGCTGCAAATGGTTTTACTGAATGGAAACTGAGATCCGGAAGTGGGTGACACAGGGTCTACCCAAAAAATATCAGTCAGGTCGTTTTGCGGAACTTATCGTTGGGATTGGGTACTATTTTGAATTCCCAATTACTTTTAAACAAACTAAATTCAAGCGCAAGCAAAAATATATCAATTTAAATATTAATTCACCTGAAGAGTTTCAAAACTAGCATTTAACTTGTATTGCTTTAAATAAGCCAGTATATTAACATCATTAGTATTTTCAATGAAACCCCAAGCGCAATACGAACATGAATTCATATTTTTATACATTTTTGGTCGCAAAACACAAACTAATTCATGTTAATCTATCATCATCATCAAATAACTTGTTTAATTTCTTACATTAAGTAAGTTATTAGAGATGGCTGAAGTGCGAAAAGTCAAAATATACTCACAGATTAGCTGGCCCCGCTTGTCCCTTTGCGACTTGGTCTTCCGGTGGACGCGTCTCTCCTCGAGGCTCTGGTCGATCTCGAGTTCGCCGGAAAGCGAGGCGTCGTGCATGTCGGAGCACTGCTTGAGGTACCGCATCAGGGTTTCGTTGGCCTGCCGGACCTCCATCTCTCCGGCGGCTATTAGCTTCCGTTCGCGTCTCCGCCGAGACTCCGACTTGCTCCTTTTGCGCCGCGGACTCTTGTCGAAGCTGCTCCTCGAACGCAGGGATCGCTGGGCGGGACTGGCCTGTGGGTCGGAGAGGGACTGCTGTTTTCTCGATGCGCCCGTGCTAGTTCCGTTTCCAGTTCCGTTTCCGGTTCCGGCGGGGGCGGAGGGCGTGGGAGAGCTCTGCTTGAGCTCCCGGTCCATGTACCCAAGTATTCCGCGATGAAAGGACGCATCGGGATCAGCAGGTGAGTGAG

 

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