CR92235

CRIMIC CR92235

Gene Tnpo

Information

Donor Location Cytology FBti Stock number
pM37-p23L:6193480..620038565A8Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK TGGAACCTGCTGAAGGATGACGAT TGTGTGCACCTGCCATTGAATCCA

Images

Construct Design Sequences

sgRNA sequence CAAGCGTAATTTAAGAGTAATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GTCGCCCTAGATCTGCTGTC AATGGGAAGAACGGGAAGG ACGCTTGAACACTGAAACCT GGTTAAGTACGGCCGGAACA
 
Amplicon 5' Amplicon 3'
GTCGCCCTAGATCTGCTGTCTGGCCTAGCCGAGGGTTTGGATCGCCACATCGAGACATTGGTGGCCAACAGCAACATTATGCATCTACTCTACCAGTGTATGCAGGACGTTTTGCCTGAGGTATGTCTCCCTTAAAACCAATCTTTTCGAAATTAACCTTTGAATTTCTATAATGTTGTTGTATTCCAGGTTCGCCAATCTTCGTTTGCCCTGCTGGGTGATTTGACTAAGGCCTGTTTCCCCCACGTGCATCCCTTCATGGCCGATTTCTTCCCCATCTTGGGTCAAAACCTTAACCCAGACTTCATTTCGGTCTGCAATAATGCTACTTGGGCCATAGGCGAAATCTGCATGAAATTGGGTAAGTGGGAGGAGCTTCTTAGGAGTATTAGGATCGTTTTAATATATATTTTGATTCAAAGGTGAGGAGACCAAGCAGTACATACGCCTTGTACTCAGCGACCTTTTCATCATTATCAACCGCCCGAACACGCCCAAGACACTGCTGGAGAACACAGGTAATTCGTTTGAGCTGATAGAAATCTTTCGAATTTCCCGAGCTATCTAAGAACTTATATGCGATCTGTTTGTATATATATTACTCTGAATGTATGCGTGTATGTGGATGTCCGGAAATTTGTTTCTGTTTTAATTTGTGTTACTTGTTGCATGAGCAGCAACGAAACGAAATCGAACCTCATTCCCATACAACATGCATAGATATAGATAGACCAGAATCGCAATTGATATTCAATTCGAATTTAGCAATCAATCGCAGCGAACTACGTATGATATTGATTTATTCAGTTGATTTATTTATACTTATTTCTTCTGTTCTTTTAGCAATAACAATCGGTCGTCTAGGTTATGTGTGCCCAGTTGAAGTGGCTCCTTATTTGCCCGAATTTGTACGACAGTGGTGTGTAACACGTTTTATATTTAAAATGCTTATTCTTTCCTTCTATTCTCTTATCTATCCTTCCCGTTCTTCCCATT ACGCTTGAACACTGAAACCTATAAGCACATCGATCTATTCCTTTGTTCCACAACTCACTAACAATCCACATCATTTAACTGGAATTTAAATTTGCTTTTGAACTTTCGTGTTTGCCAAAGCCTGCTAATTTAGTTTACTTTTCCTGAAAACGACATTTAAACATTTTCTTTGAAAAATGCAGGTGCACATCGTTGCGGCACATACGAGACAATGATGAGAAGGACTCAGCCTTCCGTGGAATGTGTCATATGATCACGGTGAATCCAGCTGGCGTGGTAGCAGACTTTATATTCTTCTGCGATGCCATCGCTTCCTGGGTTAACCCACCAGAGGATCTGCATCAGATGATACAGAAGGTTTGTACCCTACAGGTAGTAACTATTTTCTTTATTTCACACATGTTTCTTTCAAGATTCTGCACGGTTTTAAGACCCAAGTGGGCGAGGAGAACTGGCGTCGATTTGTGGAGCAATTCCCGCCAACCTTGGCCGAGCGCCTGACCACAATGTATAACATCTAAGGCTAGCCATGCCATAAAACACCCACCTTCACCCATTAGCGAGCCAAGCAGCAACTTTTTAACTAGACAATTATGTACAAGTATGCGTAATAAGCGTGTCGTCTTTATCTTTAGCCTCCTTAAGTTATTCGATGCAGTTCGAATGTTAAGTTAACATTGAAAATCTAAGCTTATAGAAATCGAAAAAAACAGCAAATCCACATAAAAGAAACTACATAACTAGATTTAAATCCACAAACATATATCGAAAAATCGAATAGAGAAGAATCGTATATATTTGAATTCAAGCTAGAGGATCTTTGATCTGTAGAACCTCTTTAGGCACACGACTGTCCGCTTACAGCATATAAAATGCCTTTCTTGTGCAGAAAACTTAGTAGGAACCACTAGAAAATTATGTGTTCCGGCCGTACTTAACC
 

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