CR92231

CRIMIC CR92231

Gene stg

Information

Donor Location Cytology FBti Stock number
pM37-p23R:29251826..2925580099A5Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK TTCCTTTTCGCATCGCACATGGCT ATCTGCTCGGTGGTGTACAGGTTCTT

Images

Construct Design Sequences

sgRNA sequence GAAGATAAGGCGGTACGCAACGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CGATGACGACCCTCCATCAG CAACGGTTCCCTCGCAATT CGTACCGCCTTATCTTCTTCTT AAACCAGCTGCTCGGCATAT
 
Amplicon 5' Amplicon 3'
CGATGACGACCCTCCATCAGGGGCAGGGCATAGGCTTTGCTGAAGTCGCCGATTAGCTCGGGCTCGTTGCGGTTCTCGGAGCGGGCCAGGGCGGACATGATCTCGGCGTCGTTCAGGGACATGCACTTCCTCAGTGGAGGGGGGTGGCTAATCGTGACCTGGCTGAGTGGGCTGGGTGCGGGGCAGTTCTCCTTCTCAACGGCGGCGCAGCGATGGCGTTTGCTCTGGATGGGCGAGCAGTTGGCCGATGCTGGCGGTTCCGGGCGCTTGAAGCAATCCCGGGCAGTCTCTGGGGTCTTTGGTGGTGGGGTGGTGGTGCTGTTGGTGTTGCTCTCCGTCATGCTGAGGCACCTTCTGACCGAAGGGCGGCGCATGGACAGACCCGCTGGCGATTTGGCAGCAGGCTGCTCCTTGATCTGGCCGCTGATCAGCGAGTTTAGGCCACTGGGGAAGCCCAGGGCGGTCTGTTGGCTCTGCGACTCCATCTCGAAGAGCTCCATGTACTCATCGTCCATGGAGGACTCCATGGAGCATGTGGAGGACAGGCTGTTGAAGATGCGGAAGCTGCGCGCCGGCGTGTGATCACTTGATACTCCCATAGCTGGCAGAATTTTCGGCTGGCGGACGATCTGGAAGCGCTGGGGCGAGCCCTCCGGCGAGAGCAGACCCATCAGCTCCGGACTGGCCGATCGCTGCTGATCCTGGGGCACAACGTCGTCGTCGTAGAACGACAGCTCCTCCTGGTCCATGCTCATCAGTTCCAGGGAACGGCGAGCACGACGAGATCCACTCATTTTGTTGATGCTACTCGAACTGCTGGTGTTATTACTGCAAGAGCAAACAAAGAGAGGGGGAGGATTAGTCAACTGTTCGGCATCGAGGAAGAGAGCTGCTTTTGCTTCCCTAAACTCTCGCAAGAGCGGGTCATTAAACCCAGCTAAAAATACTGACAAACTGGTGGTGGTGGTGGCGTTGGTGGTGTAAAAACAACAAAAATACCCGAGAAAATACAGAGAGGGGTTGTTGTAATTGGGTGAAGAGGGAGCTGAACTAGAAATTTACTTTAATGCAGCTTCGAAAACTGGAATTTTTGGGATTCGACTTGCGCACACGATATCATTGATACCATTTTTTTTTTTTTTGATAATTTTTACAATTCCCTAAAATTGCGAGGGAACCGTTG CGTACCGCCTTATCTTCTTCTTCTTGATTCTACTTAAGCATTAGCGGTTCTCCAAATGGAATTCCCTTTTGCTTTTCGCATGCATCTGCTTTTCAGCTTGACTTTTGCTTTTGGGTCACACGTCACGCAAGCGCAGCGATAATACAACAAAAACACGCCACAAAGAACCGCAAAGAACACGGAGGAAAAAAGGAACAAAAATCGAAAAAAAAAAAATGACTGACTGACGGACGACGGACAAAAAGCATTTTTTGCTGCTGCTTTTTTTTGGAGCCTCGCGGAAGCCGGTTTAATGAATGAAAAACGGCAAGCGAGCAACAAAACAACAGCTGAGCGCCAAAATAAAAATCGGGCAAAAAGCAAACGTGCCGAGCGTGATAAAAAGAACTCTTACTTTTGTTGGCCCCATCGTTTACATTTCTGTTTTTTGTATTTTTTTTTTGCTTTGCTGTTGTTGCTGTTGCTCTTGAACTTGCACTTTTTATCGCGTATTTTCAACTGGTATTCCTGGTGCAAAAGGGGTTTGCTGTTGCTAATCCTTGGGCGGCATAATTACCTGATATTGCAATCCATGCTGCAGTTGTTTTCCTCCACAATAGTTTCCCACAGCATTTTGTTGGTTTTGTTGTGTTATGTGTTGCGTTGGTTGCTTCGAAGAGCCGAAGATGTTTGATTTCGCTGGCGAGTCTGAAGCCGAAGAAATTGCTAAAACACAATTTGTTCTTGGTCACTCTGCACTCATTTACAGTTTGTTTTTAGAATTTGAGATGGTAGTCCTTGGTTTTTGGCTAGTATTGCATTTTAGTTAATTTAGGTTTTTTTGTTTTTTTTTTTTGTTAATGTGTCCGTTTGAGCATGATTTTTTGTTTTGTATTTCGGAGTGTGGTTGTGCGAGGTTTTTCTTTTTCTTCAATAATTTGAACCGCACAATCTCAATTCACCGAACGAGGAACCGAAAGGCTGAACAAGAAGAGCTGTTGACTCGACGACGAACTGAGAACGAATGTGCTGAATATGCCGAGCAGCTGGTTT
 

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