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CRIMIC CR02424

Gene Idgf6

CG5210

Information

Donor	Location	Cytology	FBti	Stock number
int200-CR-p1	2R:16691022..16693751	53D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	AAGAAGCACCTGGTCTGCTACTAC	ATCCAACTGCTGGTTCAGACTCAC

Images

Construct Design Sequences

sgRNA sequence

AAAGTAAATCTCACCGAGACTGG

 

left_200bp	right_200bp
ACTTACTTTGTAATATACTTTGCCCTCTTAAATATTATATAACCTCCAGTGGAAGTTGCTAATTAGCAAAAGTAAAATAAGAAAACGAAATCGAAAACAACGACTAGGCTTATCAACAAATACTCGGAACTCGATTTGTTTAGTTTGTTTGCAATTTGGGTCGAGTGTGGAAGCGATAATCGAAAAGTAAATCTCACCGA	GACTGGTCGTAAATTTAAAATAATTGGCATAACTAAAAGCTTTTCAATTGCAACAAAAATGTGTGAAAGAAGTCTTTATTTAATTTTAAATAGATTTCTTATCTATTTGCCATAAACTATGTATTAAAATGATGACAGCACTATAAGACAGAATCCAGGGTTTTCCCAGTACGTAAATCCCTTCTCCTAATCGCCGAATC

 

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