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CRIMIC CR02297

Gene CG2528

CG2528

Information

Donor	Location	Cytology	FBti	Stock number
int200-CR-p0	2L:21974749..21992933	40D1-40D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	no band	TGTGCAGCTCACCTGCTTAGTGAA	TCTTCCTCCAACTCTTAGAACCCC

Images

Construct Design Sequences

sgRNA sequence

CTATTTACGAACTAATAGAAAGG

 

left_200bp	right_200bp
AGTCAGAGGATAATCCAGCAGATTTAGCATCCAGGGGAGTGGATTCTAACCAGTTGATCAACTGTGATTTTTGGTGGAAAGGTCCGAAATGGCTAGCAGACCCAAAAGAACTTTGGCCTCGGCAGCAGTCTGTAGAAGAACCTGTCTTAATAAATACGGTATTAAATGACAAAATAGATGATCCTATTTACGAACTAATA	GAAAGGTATTCCAGTATAGAAAAACTTATACGTATAATAGCATACATAAATAGATTCGTGCAGATGAAAACAAGAAATAAAGCCTATTCATCAATTATTTCAGTAAAGGAGATAAGAATAGCGGAAACAGTTGTTATTAAGAAACAACAAGAATACCAGTTTAGGCAAGAGATAAAGTGCCTTAAAATCAAAAAGGAAAT

 

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