CR02212

CRIMIC CR02212

Gene beat-Vb

CG31298

Information

Donor Location Cytology FBti Stock number
pM37-p13R:12916056..1293481387D4Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CTGCGCTTTGCTGAGATTAGCATC GCAGAGGGAGAAATGAGAACAAGTGC

Images

Construct Design Sequences

sgRNA sequence CAGGACACAACTGTTGTTTGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACTGCTTTTGGCCCTTGACT TGCGGTTGTGTGAGTGTG GTCCTGTCTTTTTAGTCGCGC CAACCGCGTGCTGGTTTTTA
 
Amplicon 5' Amplicon 3'
ACTGCTTTTGGCCCTTGACTTTCATTAGTACTGGCTGAGATGTCTTCTTTCTTTTCCGAGCCCAAGAGCGAACCGGCTCGCCTGACAAGCGGAATGGGAAATCATCACTAAGGAAAATTATAAATAAAACAGCAGATAGCCAGAGTTCAAGATTTTTTCACTCGAAAACACTTATTATATAAAGAAGATATTCATTTCAACTATATCAAAACACGAAATGATTTTGGCCATAAAGACCAAAAAAATTAAATTTATTTAGGAATATACTGAAAGATAATAAACAAGACAAATCATATTTTCATTAGCTAAAATTTTAATGTGTACGCAAAATATTTATAAAAACAAAAGAAAATTAATGAACAAACTATATAAAGCGATTATCTGGTTAATTGCTTGGTAAAATCTGGTTACTATCACTTAGTAAAAGTAATAACACAAAATAACCTGAGATTGCGATATGCTTGCAGGCATACGGTCATTTTGTATTGGGTAAAATCTTTAAAAATCCGTTTGGCGCGAAATTAAAGGTAGTAACAGCCAAAAAAAATTGACACTTCATAGAGGGTTTAAATAAAGAAAGACATTATTGTATACAGTTTTTTAGATGTGCTTAGTATTGCAGCGTTTTTTCTAGTGCACAGGAGCATCAAGAAATTGACTTGCAATCTTGTCTTGGTCCTGGTTTTTCCTCCGACTCGGATTCATCCTCAGTATGAGCCGCTGGTTTTGCTCCTGGCCTTCGTTGTTAGGCAGCAAAGTTCACTCAAGTGCGTTGATTGCCGTGCAAAAAAGTTTCGTTTGTCTGTATTTGATGTACTTATTTTTATTATTTACATACACAAGCAGTCGGCATCGGTACGCACACAACCATACACACCCAGACACACTCACACAACCGCA GTCCTGTCTTTTTAGTCGCGCAGGTGAAGTTTTCCATTTACTTTGCCTCTACGGCTGTCATTAAGCTGCGGTTGCCGCTGCCATTTGCAAATGTGTTTGCCATTGTCGTCCTTGTTGGTGCTGCTGCTGCTCCTATTCTTGTTGTTCGTCGGCTGCTTTGACATGCTTCAAAGGACTCATGTCAGGGATTGGAAACCGAATCAGAATTGGAATCAGAATCACAGCCAGGCAGGCACTTTTGTCTGTTTGTCTATTGAAAGTTTAAACAAGTTTGTCTCGCCCGTTTTCCCATTTTCCAGATGTTGCACCAACTCAAAGAGTTTTTCCTCGAAGGACGAACTCGAGCAAGCCGAAAAATAAATACTCTGCTCGTGGTGCCTTCGGCAATTGCAACTTTTGACATCCTTGCAAGTTATTTCAACTGTAAACTGCGACAAATTAAATTCACGAGTGTTGCAGTTGCAACTGTTGCTGCAGGTGCTGTTGTTGTTGCCACTGCTGTTGTTGCTGTTGGTATTGCTGCTGCTGTTACCTAATGCGATTTGCGAAAATTATGCACAAGTTGTTGGCTTCCACTTGGCGACGTCTCGCGGATTTCTTGCTTATCATGCGGCAGTGTGCTCCAGTTGCAATTGCAACTTCAACTGCAGCAACTGACAGGGCCAGGATTATGGAAATTCTGTACTGGCAGCAAGTTCGACATTTAGCGTAATATAAGGAGATGGAAAAACACCGCGGCAACAGCAACAAAAACCCATTGCAGCTAATGATATTAACCATTTTTTCCGGCATTTTCCCCCTTTTTTTGCACCCGTTTCTCTCTGGCTCTGTCTGTTTGCACTTAAAAGCGTTCTGCAAATTGTAAAAACCAGCACGCGGTTG
 

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