CR02206

CRIMIC CR02206

Gene sals

CG31374

Information

Donor Location Cytology FBti Stock number
pM37-p03R:11437547..1145179186E5-86E6Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GACGATCTGCAGAGCACCAAACAA CGCGACATCGATACATACATCGAC

Images

Construct Design Sequences

sgRNA sequence GTCTGGGCGGAAGCAGAATGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AATAACCGCGTCCAGTTCGT AAAGCGGTGTTATGACCCGT GGAGTCTTTCATGCAGCATGC CAAGACGGAGATCGAGGAGC
 
Amplicon 5' Amplicon 3'
AATAACCGCGTCCAGTTCGTCCTGCAGATTGGTGGCCCGCGATCGCACCTGCACAATTTGATCCTGCAAATTGTCGACGTTCGCCTGGAGCAGCACATTGCACTTCTTCATCGCCGCGAACCGGCTCTCGTGCTTCTTCACGCGCGGCTCCACATTCGCCTTCTTCGCGGAGTTGGGCGAGTCCTGCGGAATACGGTACAGGTGAGCTGGCTAAATAGTTTACGATTCTCTGGGGCTAACTCACCTCTGGCTCGGACTCGCTGCCCGTCTCCGCCTCACTTTCCTCCGATTCGGACTCCTCGGACTCGTCGTCGGATTCCTCCTCTTCTTCGCTGGCGTGCTCCTCCTCGGCCTCCTCTTCGTCCTCGTCCTTCTCCTCGCCGTCCACATCGTCTTCGTCTAGCTTCATGGAGGCAGCCATCTTCTGCACCTCCTTCTCCAGCTTCTTAAACTTCTTGTTCTCCTCCCTGTCATCCAGAGTTGTTTCCAGTTTAAGAACAATACTACATTACAATTTGTACTCACTTGAGGATACTCTGGTTCTTCTTCATGGCTTGCTTGAGGGCGTCCTTCTCCTTCTTGGCCGCCTCCTCTTTGACCTTCAGTTTGGTAAGCTTGCTACGCAGGATCTTGAGCTCCTTGCCAGAGCGACGCTCCCTGCGCTCCACCTTCTTCTCCTCCGGCTCGTCAGGATCGCTTTCGCTGACCTCAGGCTCCAGTTCCGGCGAGCTATCCCTTCCAAAGTTTGCATCGCTTGGTCGACGCGATCCGTCCAAGGATCCACCGCCCTGCAGCCGCAGTTAATGCAAAACGGAAAACGGGAAAGAAGGGATACACACATTGGATTAGTGAGGGCATCCTTGAGCGGGGCCTGATTTTACCTGATGCCGTGCTGCATGGTAGTGGTCGTAGTGGAAGCAATCGTCGTAGTCGTCGTCGTCGTAAGCAAAGCAATTACTAATGTGCGGTCTGTTAGTGCTTGGTGTCGCCTTTGCAAGAGAGCGTGAACGGGTTAATCGGTTAGTTGCCGCGGAGTGCAATAGCAAAGCTTTCAGGGGGATAGGAATCGGGACCGGACGTGTCCTAGACGGGTCATAACACCGCTTT GGAGTCTTTCATGCAGCATGCGTCCGGCAGTTGGAGTTTAAGGTCGTCTACACACGAGATCTTCGGACATTGTTTATACCTCTCGTTTCGTTTTGATAAAGATTACATTTTTTAATGTCTACCATGGCATGGCCAGGTCCATTATTTGCTTAAAGAAAAATTCTGGAACCTTTTGAGCATTTCCCCAGAAACTTGATTTTGTTTTGTCTGGAATTGTGTGCAAGCAAACAGATAGCACAGGTAAGGGATGAAGGCACCTTACTTGAAAAGTATGGCTTCCGAAATTTAATACAAATTGAGCTTTGATAAAATATGGTCAGTTTTGAATTTCATTTTTCGCTTTCTCTTGATAATAATTTTACTATTCAACTTGTCTGATTCCTTCTCAACAGCAAGCTGTCTGCTGGCTTCAATCAGATTTCTGGTATTTTAAGTCTCGTGTGCAAACCGTTTTTAAGAAGGAAGAAAACAAGGTGTATCCACGGGTAATCCTTTCCCAGACCCATTCCACATTTGTGTCAAGTGTTGTCAGCTGATCTGTTTCTCTGGCTACAGTGGCTACTAGGGACAACTAACGAGGGATCTAAGGCGGAGTCTACGAATTGCGGTGGCTTCGTTAGCTGCTCCCGTCTACTTGCCGCCCTCAGCGGACGCAGCGAGGCGACGCCGGCGACACTAAAAACAAGAAAGAAGGACTAGCTTCGGAGTCCGATGCTTTGCATACCCTTGGAGATAGTTATGTTGTAAATGGTTGTAATCCAAAATCCTAATCTCAACTGACAACACTTTGAGCAGCATCCCATTACCTACATATACATATTACCGAACCCGTTCTGCAAAAGGAATTACCTGAAGAGCTGCTGCCAACTTGCGTTCGAGCAACTGAATCTGGCGGCGCTGCTCCTCGATCTCCGTCTTG
 

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