CR02205

CRIMIC CR02205

Gene CG13300

CG13300

Information

Donor Location Cytology FBti Stock number
pM37-p13L:6329324..635239065A14-65B1Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK ATCCCACTCCATTCACCATGATCC GACTCAACTGTGGCATACCCATTG

Images

Construct Design Sequences

sgRNA sequence AATGTTGTGAGAGTCAACACAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GTCCCTGGATGAACTGGCAA TCGTCGACTGTTTTCGCAAC ATAATGGCTAACAGCGGGCA CAATAAGCGAGGAGGAGCGT
 
Amplicon 5' Amplicon 3'
GTCCCTGGATGAACTGGCAAAAATAAACTTCACAAAATTGTTACGTGTAATCCCCTTTATGATCTGCACCCATCCATGTGCGCTGCATCTTAAGTGAAACGGGTCCACAGACCCATTCACATTACTGCTGCCAAATCCAGGCGCAGTCCTGGCCCGTTTCTCAGCTGGCTATGACCATGGTGGCTGATGTGGCACCCGGTGGCCAGTGGGTGGCTCGGGAATTGCTTGCCTCCTGTGGCAGCGATGACGATCCCATCGAGGACAACGAACTACTGCTGTTGACCGAGTGCAGCTGCTGCGGCTGATGGTACTCCGGTTCCTCGTAGGCGGCATCCTCGTCACAGTCGTGTGGATACAGCGGCACATGGTGGTGCGTGTGATGGTGGTGCGTATGCGAGTGGTGCACATGCTGTTGCTGCTGGTATGGATGGAAGTACTGCTGCTGTTGCAACGGCTGATGTTGCTGCTGCTGTGTAGCAGGCGGTGTGGTCGACGACGACGCTGGCGAGGCTCCACCAGAAGGTGGGTGGGGCAATGACAGTGGCAGAGGCAGCACCACCGGCAGCGGTTGCAGACCTCGTATGCTGTCCGCCGGGTGGGCCGTGTAGATGTTGCTCCTCCTCAGGTGGTTGTTGTTTATGTTGCTGCTGCTGCCGTTGTGGTGGTGCACATTGCCGCCGGCACCACCCGCCGCTCCACTTGCCCCGCCCGCGCCATTCAATCCGTTGCCGTGGGCGCGTTTGTTGCGAGACCACCATTGCACGTGCGTGTCTGCGTGTTGGAGAAACCGATGCAGATGCCACACAATAACAAACACGAGTGCCGAAAAATAGAGAGAAGAGAGGGTGGTGGAGAAACGGGAGGGGGTTAGTTGGTGGGTCACAGTTGCGAAAACAGTCGACGA ATAATGGCTAACAGCGGGCACAGTGGAAATCATTCATCATAAAAGGGTTAAAATAATACAAAATGTTTCACATTTTAAGATTTTACTTTGTAAAGCTTAAATCTTTGTATTTCATTTGGTTAAAAGTATTATTTTAGATGTTTTATTTGCAACTCTGAATGATTGATTATCCACTACGCCCACTGTGCTTGTCCTACGCCCACTGTTTTCTATTTTGGCCAAGTTGGCCATGTGGCTATGTCGAGTATTAGCACTCACCGTTGCGATTGTTGGAGGACTTTTTGCGCCGGCAGCGCACCACGGCAATCATGATGATGGCGCCTAGCAGCGCCGACAACACGGACACGATGATCAGAGTCAGTGGCCAGCCCAGTTCTGTGGCCTTTTCTGCAACAAAAAAAGGGGATGGGCAAAAAGGGTAACGGTTAGTAACGGAAGTTATGCAGCCCTCAGCTACCAATTCTGCACTAAGAAAAAATGTATAAAATATAATACAAATTGTATACGTTTTTTCTAAGTCTTTTAGATTGTCTAGCCAAAGCTCAATATTTAATTTTTAAATGCATATGTATCTATATTCTTTTTGTGCCAGTCAACTTTAATCAGAATATAAACCTCGAATTTGTATCCCCCAGTGCATCCCGACTTGTATTCGGTTACTCACCTATCGTTCCCTCGAACATGAATGTGCTCTTGGTGGGCATCGCCCAGGAGCACAAATCACACGTGGTCAGGCCGTCGCTCAGCACCGGCTCGTCGACGAAGAAAGGCGGGCGGGGCGGTGGCGGTACAAAGCGTCCGTTCCAGAGGCCCTGTTCGCTGGCATCAAAGGCCATTGGCGAGCCGAACAAGTCGTCATCGTCATCGAAGATGTCGAACGTCACGTCACCAAAGCTGCTCAAGTCCTCCGCGGAGCTGCTGGCCACCAGACTGGCAATCCGCGGTGGACTGAAAGTAGGAAATTCGGATGCGGATACGGAGGGCAGGGCGGATGCGGATGCGGATATGGACACAGATACGGATGCAACTTGATTTACAACGTAACCGAGACGCGCATCGTTGCTGTAGTTGCTGTTTGCTACGGATGGCATTTTCCTTGAGTTTTCCGGGTGGGGGATTGAGGGAGTTTTCCGGATAAAGGGGCAAAGCTGGAATCCTTTGACGCTCCTCCTCGCTTATTG
 

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