CR02203

CRIMIC CR02203

Gene CG31121

CG31121

Information

Donor Location Cytology FBti Stock number
pM37-p23R:24881466..2489460596B2-96B4Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CGAAAGCATTGCCGTAATCCGAGT AACCTGCTGTCCGTTGATGAGGAT

Images

Construct Design Sequences

sgRNA sequence GGAGTAGCTGGGGTAACCACTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGACCGTTTCCATTGCCGTA TGCCCATCGAAGTTTTGACG TTGCTGTTCCTCCAGTGACC GGCTGGCAAAACGGAAATCC
 
Amplicon 5' Amplicon 3'
TGACCGTTTCCATTGCCGTACTGCCTGGAGCTGCCGTACAGTCGGGCTTCGTTATAGTCGTAGCCCATCCCACCACCGCCGCCATCCACCTGGCTCAATCCGGCCCCCAGAAGATCCGCCTCGCTGTGCGATCTGTGACCGTAGTGCTTCCCGTACTGCTGTTGATGCTGCTGCATCTGATGGTGGTGCTGACCCACCGCGATGCTTGCTCGGCTGTTTGCCTGCCGCAGGGCAGCCATCGGGACACCGCCTCCTCCGATCTGAGCCCGCGACTCAGGACGCGAGTGCGGCATGCCGCCGATGGCACGGAAGTCGGACTCGCTGCGATACTTCCGGAGATTGGGCGGCACCCGACTGCGGGTCGTCTCGTTGTCGCTGCTATCGTAGCCACTGCTCCCGGCTCCAGCCGGTGCACCCGAGCTATCCCGAAAGGCGCGGTTCACTCGTCCTCGCACTCCGCCCAGGGCACTGGAACCACCATGGCCATGACCCGGTCCCGCACCCGGACCCGGACCGGGACGATGCGATCGCTGCGATCCAGCATTTGGCGGAAAGACGCGCGGCAGTCCGAACTTCAAGTATGTGTACATATTTGAGCCCAAGGGAGATCTGAAAGGATTAGGTATGGTCGGATATATAGTATGGTGATAACGTTTAAATCTTATGCTTAAATATATAAAGGTATAGCTAATTCAATTAATGTGTTTTTTGTACGTAACCGAAATTCTCAAGTGCTGAGATCATACATAGTCCGGAACTATTAACGCTTGATTATGCTTAATAATTGTTTATTTAAATAATGTATGAGATTACTCACTTGGGTCCGTAGCGCGGATGGTTTAAGATTGATTGAACTGTGGTATCGCGTAGCTGGAAGTTTCCATATGGCAGCGGTGATTTGTCCGACGAGCCCAGAGCTGAATCGGTGAAGTCAGAGTTCAGGTTTTGCCTACGGATGGGATGGGATGGGGGTATTTGTATTGCTCGTTGTGGAGGATGGAAAGCGTCATAGAGAGCTGCACTGGGCGGCTGCAGTTGCGGTCAGCTGGACATGATTGGTGGGCTTGGCCGTGGGCTCTTCAATTAGCCATTTATGGCATTTGAACTTCCGTCCACTGCGGTTAAATCAACCGTCAAAACTTCGATGGGCA TTGCTGTTCCTCCAGTGACCACACCCAGTTCTCGAAATGGGGTTCCTCCACCGTTAACCACTCAATCACTCACCTGTAGATGGACCAGGCATGCAGATCCTCGGATGCCGCCGGTGGCAGTGGGGTATTGGGTCCGTGGGGCACCGAGTAGCGGCGCTCCTCGCGTGACATCTTCTTGCCGCTCGAAAAGTTGCTCAGCTCCATGATCTGCCGGAAAGCACACGGATCGGGTTAGAGAGTTCGGGCCAAAATATTAAGCTAATTCCGACTTAAAGTGGGTCAAGAATGACGCGAGAATGGCTGCGCTATTATTACTCATAATTGGCGGGAATTAGCCTAATGACAATGCCCACACATATCCACACGCAGCGCCAGTTGGAGTCGAAGTTGGATTCGGAATCGGAGTCGCAGTCGCATATTCACAACCATTGTCATGACAAAAGCGGAAATGGTGTCGTATATGAGGCGAGGCGAACTTAAGACACCCTAACAAAGACCTTTTCGAAAATTTGATAGATACTTTATCTACTTTTCATAATAAATAAATCAGAAAAAAATAATGTCTGTGCTGTATTTATTAACTTACTACCCATGGGTTCTTTTGTAGTTGTGCAATATCATATATATTTTTTGTGAGTCTTAATCCGAACAGGTGCACAAAAAGCCACCAAGACAATGCTAGCTAATCGGGTGTTGTCTTTCAGATTTCCAGCTCCGTATCTTGAGGAAATCCAAAACACCGGGCGGTAGAACAAAGCGCAGCTCATTTGGACACGGCCTCTGGGTTTTTAATTGCCAATGCCCGCCGAAAAAAAAAGGTAGCAAAAATCCATGCAAATGAACCAATGTTTGCTCGGCAGCATTATGCAATGTTAAGCGAACGAAAGGCAAACAAACTAATGCAACTGCTGTCGCCGGATTGTGTGGACGCCAAATGGATGCAGTGCAGCGAGCCGTGCGGATTTCCGTTTTGCCAGCC
 

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