CR02196

CRIMIC CR02196

Gene CG2616

CG2616

Information

Donor Location Cytology FBti Stock number
pM37-p23R:7722417..772471584D13Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short OK GGAAGAGCTAACGATGGAATGGCA CATGTGTGGGAAATCCCAGCACAA

Images

Construct Design Sequences

sgRNA sequence AACACAATAACAGCTAGCTGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAGGAAGACTCAGGCGCATC TCCATTTGCTAAACTCTGCAGC TCACAGAGAACTACCTGGGGA CCGGAGACTTTAAGCTGCGA
 
Amplicon 5' Amplicon 3'
AAGGAAGACTCAGGCGCATCCAGTGCAGAGGCGAATCCAGCTGAATCGGAAATTTCGATGGGTCGAGGACCCCGCCCAAGGTCCTTTACTGGCGGCGGAGGCGGTGGCGGAACAGGTGGATCAGGTGGAAGTGGTGGTACCTCCGGCGGCAACAATCTCAAGCCCGAAAGAAGCGCCCGGGAAGATGACGCCATCAACCGTCTGACGGACTCGAAGTCGAGTCACCGAAAGCTGCTCAGCGATCCGCGATTCCAGATTCGGCCGCTGCAGCAGGTAATCTCGGCCTGCACCGGGGCCATGATCACGGCCTGCTTCATGACGCCCCTGGACGTGATCAAGACTCGCATGCAGTCGCAGCAGTCGCCGGCCCACAAGTGCTTCTTCTATTCGAACGGGCTGATGGACCACCTCTTCGCAAGTGGACCCAATGGCTCCGAGTTGGCGAGCCTGCGGCAAAGACCACAGTTTTCCAGCTCCTGGGATGCTCTGATGAAAATCAGCCGGCACGAAGGATTGGCCGCTCTTTGGTCCGGCCTGGGTCCCACCCTCGTCTCCGCCCTGCCCTCCACCATAATCTATTTCGTGGCCTATGAACAGTTCAAGGCCAGATACCTCCAGATTTACGAGAGCCACTATAATAAGAGTCAGGAGCCCCGGCACCTTGAAATCAGGTACTTACGTAACCCTTTAAATACATAGTGTAATACATACATGTAGTTTACCTGTTGAAGCGCTCATTAAGCAATTCTTCATAGCGAATATTTACATATCCAGCGCATGAATAACCATTTAGTTTCAATAAATTGTAAAATCGTTTTAGCTTTAGATAACAAATAAGCTGCAGAGTTTAGCAAATGGA TCACAGAGAACTACCTGGGGAATACTAGTTTAAAAATATTTATTTTTCGTTTCATTTCGCTATTTCAAATGAAAAACATCTAAATTAAGGTTTATCAATACACATGCACATACAATACACATATTTCCACTGTATTAGACTGGATAGAAAATGTTTTGATGTATTGTACGATTTATTCGCAACTTCACAACCTATATATAAACCTATATAAGAATATGATTTAAGGATTTACAGGTTTAAACGTTTTCCATACTTCTAGGGATACCAAGAAAAGTCTACCGTCGGTGGTTCCCATGATGTCCGGGGTGACTGCTCGGATTTGTGCTGTTACCGTGGTGAGTCCCATCGAGCTGGTGAGAACCAAGATGCAGGCCCAGCGCCAGACCTACGCCCAGATGCTGCAGTTCGTCCGGAGTGTGGTGGCCCTTCAGGGCGTGTGGGGCTTGTGGCGGGGCCTGCGTCCCACGATCCTCAGGGACGTGCCCTTTTCAGGGATTTACTGGCCCATCTATGAGAGCCTAAAACAGAATCTCGGCCATGGCTCACAGCCCTCGTTCAGTCTGAGTTTCTTGGCAGGCGTGATGGCAGGGACAGTGGCCGCCATTGTGACCACTCCTTTCGACGTGGTCAAGACACATGAGCAGATTGAGTTTGGAGAAAGGGTCATCTTCACGGACTCGCCGGCGAGGGATTTTGGAAAGAAGTCGACCTTCAGCAGACTAACGGGTATTTACAGGACACACGGCGTGCGGGGACTCTTCGCAGGATGCGGACCCAGGCTTCTGAAGGTGGCACCTGCCTGCGCCATTATGATCTCCACCTTCGAGTACAGCAAGTCCTTCTTCTTCCATTACAACGTGAGGCATCATAACGAGGCCCTACTCCTGGACAACCCGAAAGACACGACGGTCGAAGATGATGACATTGAGTAGAGAGATGGGTGGCTCCTTTGTAAATAAAGAGCGCATATTTTATGTAATCCCCGGGTGGCGAGGCGAATTCAATATCCCCAACGCTTTACGAGTTACGTGTTACGTCAATAAGGCGAAGCCGGGAAATCAATTTCCATTTTCGCAGCTTAAAGTCTCCGG
 

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