CR02195

CRIMIC CR02195

Gene CG13407

CG13407

Information

Donor Location Cytology FBti Stock number
pM37-p23R:22103414..2210559094A2Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GTACGTGTTACGAACAGTGATGGG GAGTTTGTGCCAGCATACGGGTTA

Images

Construct Design Sequences

sgRNA sequence GGGGGGGGGGGTTGGGCATGTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TCAGCACCACCACGATTACC ATGTGGTTGTGTGGCCGAG CCCACTCACTCATCCGCTTG TGGGGCCGATAATCGTGATG
 
Amplicon 5' Amplicon 3'
TCAGCACCACCACGATTACCACCCACTTCAAGTTCACCTGCGTTAATAGCCGCCTGCCAGGCAAGTGTCTGGGAAAGGCTTTGCCCAGTTGCCTAAACGACATTGTCACTTGCGGTTGGTTGGGCGCATCATCTCCTGCTGCTTCAGCCCCAAACTCCAGTTCGTCGTGCTCGTCCTGTTAGTCCTGATCGTCCTGTTTGCGGCTTCTGCAGTCGAGTTGCCATCGGTTTATGGCAGGTTGAGCACCAGCGACAATGAGCCACGCTGGTGCACCGTGTAGTGATAAACATGCGCGCTTTTCGGCACAAATCAATTAGCAATTGAATTTGGTGAACTCAAAGACGGTGAAAATGTCCGACGGCCAAATGACAAATCAACTCAACGTGCAACAGCAGGAACAACATCCGCTGCTTTCAGCCATCTCAGGAAATAATTATGCGTAAGCCATGGTCAAAGGCATTGGGATGTCGACTGTATTTGCCATACAGCCAAATCAAATTAAATTTGATATAATTAAGAAGTGGCACACTCACTCAGTTCCCTAGAATTATATTGTTAATCTGAACTCGCTTAAGTGCTTTTTATATATTTATCAAGAAGTCCTGCTAAACAACATCGTAAATAATGAATTAATTCTTTTTCAGTCATTGATATATGCAAAACTTAAACGCTTTAACAAACTTCTTTCTTCTATCCTACGTAATATTAATAATCGCTTAAAGGAGCCTTGAAAGAGTTTAGTCAGTTGCAACAGGATAAAGTGTGCTCTTTGGCTTAAAGATTAAGTGCTGGAGTGCCGGAGGCAACTGCAATCGCAAACGACCCCTGGCCCAAAAGACAGCCACTGCGGGCGCGGACTGATACCTCAGGCACTTAGCCGGGCTAATGTGGGACACAAACAGAAGCGGCGTCTCGGCCACACAACCACAT CCCACTCACTCATCCGCTTGCCCGCTTTCACCACTCACCTTCTAACCTTCTCTTGCAGTCAAACGCAGCGTGTGCATTACTATCCCAACCACTACTACCCGAGCCACTACTTGCCGGGCCATTACCACCAAGGACACCAAGGATACCAGGGGCAGCAGGGACGACCACCGCTCCTGGCGGCCGCCAGCAGCCACAACGGTGCTTATGCGGCCACGGCGGCTGGCAGCGGACACTCCGGCTACGGGTAAGCAAAGAACCACCGCTGCACTGAGAGAAAAGCAATGGGATGAACAAGAAAATTCATTCCAAATATACAAAGAAATTAAACTGTTATGAAAAAATATAAATAACTATTTGAAGATGAATATATACTTAATAATATTGATACTAATGAATACTAAAGTTTCGAACTTATCAACCTAAATTACCTATTTCTTCCTGTGCACTGGCCATCAAAGTCAAACCATAACCCCAATTCCAGTGCAAGTTCCGCATCTAATGCCAGTTTGAATTTTGCAGCGGCATAGAGCCATCCGTGGAGTACGAGCTGCAGCATGACCCACCCTCGGGTAGCTTCTCCTCCTTTCATCCCAGTAGTGCGGGCAGTGGGCCCGCATATCCATCTGCCCACCATCCGCCGGCTGGTCCACCACCGGCTCCTCCGCCCACCAAATCGTCAAAGAAATCGAAGAAGAGCAGCGGTTCGGTGATGAACGCACTAACGCTGCTCTCGTTCTTCTTCTTTGTTAACATGCTGCAGAACTGCCTGAAGGATCATATGGCTGACATGAATCCCACCGTGATGGTGCTCACAGCCAGCGGCACTCGCAATCGTTTCAACAAATTAGCCGAGATGAATTCACGCGAACAGACCTCGACAACGGCCACCGAATCGGTAACGGCATCGGCAGCGGCATCATCTTGGAATCATCAGGCTGCTTTAGTTCCGGCCGTCGAGCCAAGTGTGCCGCCCTCCATTGTTACGCCCACGGTGGTGCTGCAATCGCCCTACAGCCGTCACACGGAGATGGCCAATAAGCCGCCCCAACGCCAGCCAATGGAGTACCAGTCGAACCCTCAGCAAAATCATCACGATTATCGGCCCCA
 

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