CR02194

CRIMIC CR02194

Gene CycG

CG11525

Information

Donor Location Cytology FBti Stock number
pM37-p23R:31596642..31612664100C7Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK ATCAAGCAGCTGGACTTGAAACCC ACTGCTGTCCCATTCCAGATGTTC

Images

Construct Design Sequences

sgRNA sequence CAGCTGGCTAAGATATACATAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGATGGTCCGCAGCATTCTT AGCTGTTGTGCGTCCTTGAA GGTATTCGAGTATATGCCTCACA TGGTTGTACCGCTGGTGATC
 
Amplicon 5' Amplicon 3'
TGATGGTCCGCAGCATTCTTTTTAATTTTTTTTTTATATTTTTTTTTGCTTTTGTTTTTTTTTCACGTATTTTTTTGTCTGCCGCAAATAAATCGAAAAAATGTGGGGGCGCTACTCTCGGGTAACTTTTCAAAAGGGGTGGGCGTGGCTGCGTCACCTTGGCGCCCACATTTCCGTATCTCAATAGTTTCTTAAATGTTAGAACCAGCGCGCTACCAAATACACATTTGTGTGTATGCCAAAAGAATTATGATGCACTTTAGCAAAATATACACACTCTACCTCAACCAGCACTACTACACTCGCACGCGGAACGCTCTCACGGACACACATCCTCTGCGGAGATTTCCACCCTCCTGCTGCTGCTATGGGATATGCCTAGTGCCAGGAAAGCAGGAGGTGTGAGGTGTCAGGTGTGTCCCGCGATTACGTCTGGTTGATGGTCCTTTTGTCCGCTGCTCCAGTTTTGCCCAGCTGTTGACCCTGCTGCTCTACTGCTGCTGGCTGTCGCTGGATACTAACATTGTTCGAAAATTGGAATTATGGGTGAAGTGGGCCAGTCCTCCTCCTCCTCGGAGCTAATACTCTCGGTTCTGGAATAAGAAACAAACAAATTTGCAATGTTAACAAAAAAAAACGTATCATACCAGTCAACCAGTAAAAGTGATTTATCGACCATTTGTTGAGTAACTAAAAAAAACCTTCTATCTAATACCCCCGTTTTTTAAGGCTTCTACCTTTTCATAACAATCGGACATCAATTAACAAATTAAGTTCAAGTTGAAGGCCCCTTTTGCAATCAAGGCCCCGAAAATCAGCTGATAATTCGCAAGCCTTTGCTCAGGTGCAACTGGAACACAAAGTTCGGGGAAACAGTGCATTGAACTTTTCGAATGTTTATGACGAACTTTTCGAAACACCGACAAAACCCTAGAAAACATTATCAACGATAAGATAGCAATTAGGGGGAAAGCTAGAAAAGAAAGAAAACAACCGGGCCGAAATAGAAATCCAATGACATTGAACATCCACATCTAAGTTCAATGTGAGAATTCAAGGACGCACAACAGCT GGTATTCGAGTATATGCCTCACAGATATATATATATATGGGTTTTGCATAAGCGAATACTCTTTCCGCGACCCTTGCTGCGGTCTACGTGCGTGTATTTTGTTGTTGCGTCTAGCGGGTATTGTTGCCGATTGTTGCCGAAAAAAAAGGCAAAAACAAGGGCAGCGAAAATAAAAAACAAATTGCCACACACACAAACACCACGCATACATATATGTATGTAGAATGGGCGGTTCGCAGAGGGGGCGGACAGACGAACGGACGGACGGGCGGTGGGCGAGCCAGCCAGTCTCATGACAATTGTGTTGTTGCCGCTCTGGCTGATAATGGAATCGCTAATATTCTAGAAACTTGTTTACGCCAACAAGTTCAGATCGTCATCGACACATTTGCTTACATTCGAGATTATATATGCGCGTGTTGGCTATGTCCAAAATCACTAAAAATAAAGTCCGGTGATCCCTGCCGCAAAGTTCTTTGGTCCAATTTACAATTATGTTTGGAAAACTGCCCCTCTTAAGATAAGATCGCAGACTCACCTAGAACGCAGGCCATCGTCGAGGGCGTTGGGGATGCCTTCCTCAATGGTGGGCAGGTCGGAGGAGAAGCGGTTGATCGGGCGCAAGACGCGCAGCGTGCGACTGGACAGCTTCCAGACAAGCCGCTGCTTGTAGGGCGCCTTGTTCTGCCCGTTGTAATGGGACAGAATACCCGAAACGATGCTGAAGCCGCAGGTGAAAACGCGATCAGGAATCTGTGGGGCACACGAAGCCAGGACATTAGAGAGGTTCAAGTGAAAGTTCCAGTTTCTCAACGAAACTCACCCTCATGTACTGCTGCAGGAAGAGAATGTACTCGAAGACGTGCTTCAGCTCCGGACTGCCGCGGGTGTACGACTCCTTGATGTGGAAGTCCAGGTGCAGGCAGATGAGCACCAGCGCCAGCGTCGAGGGCGTGATCACCGTGGTCTTCACGTCGCACATCAGGATCTCCAGGCGGTTCTCCAGTTCCTCCAGCTTGATGAGCTGCTGGTAGAACTTGAAAAAGTCGCCGCCGATCTCCTTCGCCAAGTTGCGGAAGAGGGCGTAGTAGATGCGCAGGTAGCTCACAGAAGTGATCGGTGCATGTCCCATCTGGACGCCCAGCTTGTTGGCAATCACGCCGGCCATGCGTTCCAGATCACCAGCGGTACAACCA
 

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