CR02187

CRIMIC CR02187

Gene CG5854

CG5854

Information

Donor Location Cytology FBti Stock number
pM37-p13R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GATAAGCGGAAGTGTGGAGTGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATACAGCCATCCGATGAGCG GGCAGGATGACGATGAGGAG AGCATCGCTGACTGCATCG GGGTATCAGCGAAGAGGGAC
 
Amplicon 5' Amplicon 3'
ATACAGCCATCCGATGAGCGACAGTGCTTGGACGGACGAACACCGTTGCGCTTGCGCCCGCTATAGACCTTGGTGAAGGCCCCAACGCCGGCGGGACTGCGCAGGTACAGGTGTCTCATAATAGAGGCGCAGCGAGTGTAGAACCAGTCGTCGTCGGTAGGAGCAGTCTCCTTGAACTTGCCAGTCTTCATATAGACGGCCTGTTCGGGCACAAAAATTTTTCCGGATTTTTTGAGAAAAGCAGCCATGTTTTTGGTCAGAACGTGCTGATCGATTTCCTTTACTGTGACTCCAGGCATGTTGCTCTCTACGTTCTTGAAAGCACGTTTTTAAGGCTCATGGGTCCGATATATGTATGTATGTATTTAAAAAAGTCTTACCTTTTGAGAAGAAAAAGGTTTGCTTCCGGTTAGTTTGCTGACTTTAGTCAAAATGGACGATTGGCAAGTGACGTTTGAAAGAGGGACCAGGTCATTGACCCCAAAGGATTACTTTCCGATAGTTTTGTCGTGCAGTATTGGTGAACTTGGCAATTCTTTTCGAAAACTTAACCTATAAACTTGGAAAGGAACGCAATGTAGCAAAACTACTGTTCTTGGTGACAGGGGGTTTAAGGTAGACTAACAAGGACAATTTTATGACACTGAAGCCCTATGGAGTAAGAATCAAAGAACTGCTGTATTTTGGTTTGTATAAATGAATAAAACGTTCTACGCTAATTGAAGAGCATTCGAAGAGGTTTGAATACAGCGCCATAGGGTGACCAGCTTGTGGAGCATTGAAGGTATTTCTTGTTTTAAGAATGATCACGGGATGGTCACACTAGAAATACAGCCAAACAAAACAACAAAAAGCATTTCGAGCGCTAACAAATATATATCTTTCGACTTGACTCATTCGCATTCCGGTTGACCGTGTCGCGCCTGCAGCATGTCTGAAAAGCCGACTGTTCTGATTTTGGGTGGTAAGTGGTCCCGCTGCATCCTCCTCATCGTCATCCTGCC AGCATCGCTGACTGCATCGCTGCTCTTCCTCTTCCCGCAGGCTGCGGCTTCATTGGACGCAACTTGGCCACTTATCTGCTGGACAATGAACTGGCGCAGGAAATACGACTGGCCGATAAGACCCCTCCGCAGATGGCATGGCTAAACGAGGAGCAGACGCGGGTCTTCGAGAGCGATCGGGTGGAGTTCTGCAGCGCGAACCTAATAAACGCCGGTAGGTAATCACTACATTAGGGTGGCTCGTTCCACCAGGATGCGGATCACAACTCCTATGGAGATCCAAAATCCGCAATTAACATTCTTGTGAAAGGAAAGCGCTATCGGATATAGAATTTTCAAGTACCACCTTGGTATCGCTGCGCGTAACGAGCCACCCTAATGCATTTGTTGCACCTAAACTGGTTATAGAACGCTTCCCTTTGTTTTCATACAGCCTCATGCAAGGCGGCTTTTGCGCCACACCCCACCACTGGACGGGCCTGGGACATTGTCATCAATTGTGCGGCCGAGACGCGTGCCAACCAGGATGATGCCGTATACAAGGAGGGTATCCTGAAGCTCAGTTTGAACTGCGCCAATGAGGCGGCTAACCAGCGGGTCAAACGATACGTGGAACTCAGCTCTGGTTGTGTGAACAGCAGCGAAAAGACGCCGTTAAAAGAGGACTGCAAGACGGATCCCTGGACGGGCGTGGCCAAGCAGAAGCTCAAGGTGGAGAAGGAACTGGCCAACATCGACGATCTCAGTTACACGGTCGTCCGTTTGCCCGTGGTCTATGGCATTGGCGATAAGCGCTATTTGAGTGAGTGTCCCTCTTCGCTGATACCC
 

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