CR02173

CRIMIC CR02173

Gene m-cup

CG11896

Information

Donor Location Cytology FBti Stock number
pM37-p23R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band short band CAACAGTCGTCTGTTACAGCAGCTGT ATCAACAGTTTCGTCGTATCCAGCCG

Images

Construct Design Sequences

sgRNA sequence GTCTTTGCGTCCCTAGACGAAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCATGGACAACTTCCCCGAA CGAAGGTCCTTTGCACGAA GCAAAGACAGATGGTGGCAT TATCGCCTCCGGTACTCCAT
 
Amplicon 5' Amplicon 3'
CCATGGACAACTTCCCCGAAACGACGGCCGCCCATAGTGGCGACACATAGTGAAAGCTATTGTCCTCCGGCACCTCGAAGTGGCCCCTCTGTTCGATGTTCGCCTCGCAAATGGTGATCAGATACTCAGCGATGTCCACGGCACCTCGTTTACAGGCGATAAAGAGGGGCGCGCAGCCATTTCGCTGCTTTCGCACCAACTCGCGACGAGAATCGCGGGGTAGCCTGAAAGGAGATAACGAATCGATGTAAATAAACTTTAAAATAATTATTATTCTTCGAATATTCTTCGGAATTTCTTTCTGTGCAAGGTAGGGTAGAGAATCGGAAGCTCTTGATCAGCACGTGCAGGGGCAATGATTTTGTGACCATTTGTTGATAAGATCGAGCTTTCAGCCGAGAATTCACACATTTGAATTTAATCAGGTATTTGTGATTGCAGAAAATATTTGGATGTTTACAAAAGCCTTAAACCAATGAAGCTATATATATATATATATATATATATATCAAATCAACAGGACAGTCATTTATCTATTATAAAATCTGCTTAATGAGGTAATCAAATTTCGAGAACGATTATAACATGTATAATACGCAGTATTCGTAAGTCTATAACGAAAGATTAAGTTATGAGGTGCGTTGTTTTGAAAAAGGTGGCTTCCCTATAATTTTAATACTTTATTTATCAGTAAAGTCAGGCGATTCGATTAGTTCCATTTGTCCGTCTGTTTAATATTGGTGTTTAGAGGTAATCATAACAACACTGACCAAAATCTTTTGTAGAAAAAGTTTAAGAAATGAATTGTTTCTACTTATGGAATAGTTCGTGCAAAGGACCTTCG GCAAAGACAGATGGTGGCATTGTAAATTATTTATTATAAGTTTCAGCCAAATGTTGTTGCTTTCAAATTAGCAGGAATTAAAGTACTGTATCCCTAAGATGGAAGTCAAATATATGCTGGTTTCACTGCAGACACTATTATTATTGATTTAGGAAAACCTAGTTATTGGTCATTGAAATGAAGAATGGTATGGAATGGAGCCACGGAACCATTTAAAGGACTTGTTCATTACGAAACGCTCTACACGTGGAAGCCAATTACAGGGACATACTTTTCTAGTTCGTGGCGCAGGTGGCGCGACAGCAGCGAGTCCTCCTGGCTGCGTTTACATTCCTCGATCAGGTCGTGACTGAGCTTTGTTAGCTCCACCTTGGGACTGCTCTGCTTGCGTTCGTTGGCCATCGATGGCCGTGCGACTGACTGCTCCTGCTCTTGTCGGGTATTGAGCTCCTGATCCTGATCCTTGGCTTCCAGAACCAGATGCAGCTGCTCGACCTGTCGACGACGGCTGCCTTCAAGTGAGCGCATTGCCAGCGCACCGTCTCATTGCCAATTGATGGGTGGCTGGCTCTATATCCTTTGCAGTGGGTGTAGATGGGTGGGTGGTGGATGGTGTATGGCCTCCTCCCGGATGTCCTTGGGCCACTGCCCCCTTGCGAAAACGGTGCGGCAGTGACCTACTGAACCCACCAGCCTGGGCTACTTAATTGGGTTGCTTATCGCTGCTCCCGTTTTTCAGCCCAAGGCCGTCCGAACACTTTCTTTTTCTCTCTCTGTATCTCCGCTTGGTTGCCTTTCCTTCTTCTCTTCGAATCAATTTCTATGCAGCGTAAAACTTGGGTGCCTAATCTATGGAGTACCGGAGGCGATA
 

Search Again