CR02161

CRIMIC CR02161

Gene tow

CG14821

Information

Donor Location Cytology FBti Stock number
pM37-p13L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK no band

Images

Construct Design Sequences

sgRNA sequence CATAAACGACCCAAATATTTCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACGGTGCACCAGACGTAAAT ACAATGGCGCCTTTGTGTATG CGGAGGCGCCTAAGCTTTAG TCACTAAGTGGACTCTCACCTC
 
Amplicon 5' Amplicon 3'
ACGGTGCACCAGACGTAAATGCGTTAAAGATGAAGCAAATCAATAGGTTATCTAAGGGTTTTCATAAGCCCAAAATACACAGTTCAAATATTTTTAGTAGCCACCTATGTGCATATATATAATAAAAAATCAGCAATACTGATAAATTGATCTTCAAAAATTTCTCAGTTCATTTTACTTATAGATTTTATGTTATTCTCATTTTGCGTGCTAGAATTGATATTGTTTTGCCAACTTTGTGTATGCCCGATCATTTGGAATGATCTATCAAATCGTTTAGGCATTTTCCTCTGTGTAGAGCAAGGGAAATTGGGGAAACGGCGAGGAAAACGTATTGCCTCGCACAACAAAAGCTTACACGCATGACGACATCAATTTGCCTCCAGCTGTGTGTGCATGTGTGCGTCCGTGTTTCCGTGCATGTGTGTGTGTGTGAGCCACCTGTTTCCACACAATGAGGACATCATTGATTAATATGTCACCAGCAGTGAAAAAAGAAGGGGAAGTTGAAAGGAGGAGCCCAGTTTTAAGGCCATACATTCACCAAAGTAATAATAAAGGCCTACATTTTAAAGCCCACAGCATTGTCTATATATAAACACACACACACGCACACTAGCCAATTTAAGTTTATAAGAATGTAAAAACCTAGCCTACATGGCAAACTTTTGACTATGCCCAACCCCGCGCGTTTGTTGCTGTCTGTGTCTATGTGTGCGTGTTTCTTGCCTTTTTTATTTCATTTTGTGTGTGTATAATTAACTGCTAAAAAGGCAACTTGTTGTTGCAGTGGGCGCCGCTTCAAGGGGCGTGGCCAGCCCCCCCCCCCCAGTTTGTATATAGCTGTGTGTGTGTGTGTCTCTGTGGGCAAAAGGGTTAAAGCTAAAAAAGTATAGCATACACAAAGGCGCCATTGT CGGAGGCGCCTAAGCTTTAGTCTCGTTCCTAGATTAATTATTCATAATACTCGCGGCTCTCGCTTTACCGAATTTGCATTAAAATGCATAACGGCTTTTAATTAAATTTATGGCTGGCAAGGCCACTTCAAAGAAAGGCCCGAGGAAATATTAAGTATACGCAGGCTAAGCCGAAAGATGGATTAGCAAAACAGAAACGTGCAGGTACAAAAAGGATGCTAAAAACTTTGATTTCTTTATTTATTTTTATAAATAATTAAGTATTTGAAAGGCAGATAGTGTTTTTTTGAGTATCCATAGTGGCCCAGGACCTAGCATAATTTCTACAAGTGTTTTCTAAGCATTTTTCTCGACTGCGACTGCCCCCCGTTTCGCAGAGAAGTTTGCCACACTTGGCTTATTGCAAAACTTTTGCCACTCCATGGCACTCGCGAAATTGCCCACTCATCTTGACTAATGCTCGAAACTCTTCTTTGGCATTATCCATTTGTTTGGCAGCCGACTCAGATGCCGAAACGGACGAGGACGAAGGCCATATCGAGGATGCTGAGAAGGCACAGCAGCGCGACCGCGAGAAGCACGACGAGAGCGAGGAGCTAAGCAATAAGGACATCCAGGAAAGCGACGAGGACGTGGCCGTCTCACTGCTGCGCGCCAAGAACCTATCGCTGCTGCAAAGCCAGTAAGTTAGCCCATCGACCGCATAACCCGAAAATGAACACCTCACTTTTCCAGGGAAATATCATCTAGCCAGCAGAACTTCTTCCGCATGCTGGACAAGAAGATTGATGAGGTGAGAGTCCACTTAGTGA
 

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