CR02156

CRIMIC CR02156

Gene Pp1alpha-96A

CG6593

Information

Donor Location Cytology FBti Stock number
pM37-p13R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK

Images

Construct Design Sequences

sgRNA sequence TGTGGGGAATGGGTGATGATGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTCGAAATCGCATCCGCAAG GGTGGGGGCAAGGTAATCAA ATCACCCATTCCCCACATGC GATGCGTCGTATCTGCTCCA
 
Amplicon 5' Amplicon 3'
CTCGAAATCGCATCCGCAAGTTTGTCGAAAAACTGTGCTCCGCCAAGTTCTCGGAACGCGCGGAAATGTTGCTGAAGCACAAGAGTCCACTCAAGCCAATGGAGGTTCCAGGCAAAGGGATCCCGAGGTCTGCGATCTCTGAAAAGTCTGAAGAGGATATAGCCAGCTCTCAAGGCGAAGAGCAGACGGATGAGCAAACGGATAGCAGCGAACAGCAAACTCGAAGCCCCAGTCCTCAAAGACTAGTTAGTCGCGATTTCAAGGCTCGTCTGGAGGAGATCCTGGTCAAGCCGGCGGCCACTATTCGAGGAGCTTCCAAGTCGTCCCTGAGTTCGAGGCCAGTTCCGCTGCCCAGGAAGCGGGTGATGTTCAACACCACGGAGGACGGGAAGAGCTTCAATGACAGCGATGATAACCTAAAATGAGTCAATAAAGATGATATTATAATACTTTGGGTATAAATGTCAACGATACGTTTAATAGCCAATATATCATAAGATAATTATATTCCTTTATGAGTACCTATCACCTGTTCAATGTACCGTACTTAACTAAAAAATATTGTTTTTTAAATGAATTTGCATATTCCGGTCACACTACCTTTTACCGATAGCTGCGCGCCGTGCAATGGATAGGCAATCGATAGGCAATCGACTCGCGCTGTAAACAATCGACGTCACTAACCAACGTAGATGAAAATTTTTTAGTACGTCGCGAGTAAAACGTGCATTAAATGCAATAAAAGGGCAGCTGGCTAAGGATCGGAACGATTGAAGTCAGCCAACCTCAGAGGGGTGAGCAACCGAGGCAGTGAATCCGCGAATCCGTGCATCCAGCTGACCAAAGGGCGAGATTAGCCCAGCACCGAGTTTCCATCCGCACACGGACACGGCGCACACACACACAGGCACACGCAGCCCGTGCCCATCGATGAAATAACATACATAACGAACGTATACCGAAAATACACGGGTTACGGGAAATAGCAGTTGCTGCAATCGGCGGATAAGCGAACTTCAGGAAGCTGAGCAAGCGGGCCCACAAAGATGTCGGATATCATGAACATCGACAGTATCATCTCGCGCCTGCTGGAGGGTAGGTGTTAGGGCTCCAACTTTTGATTACCTTGCCCCCACC ATCACCCATTCCCCACATGCCTTGCCACCCAACCACCGCCCACTCTCATCACCTTAACGTAAATTTTCCCTGCTTGGTTTTCCATTGCAGTGCGCGGTGCACGACCGGGAAAGAATGTTCAGCTATCAGAGAGCGAGATCCGCAGCCTGTGCCTCAAGTCCCGCGAGATATTCCTGTCGCAGCCCATACTCCTGGAGCTGGAGGCACCGCTGAAGATCTGCGGCGACATACACGGACAATATTATGACCTCTTGCGCCTGTTCGAATACGGCGGCTTTCCGCCCGAGTCAAACTACCTGTTCCTGGGCGACTATGTCGATCGCGGCAAGCAATCCCTGGAGACGATATGCCTGCTGCTGGCCTACAAGATCAAGTACGCGGAGAACTTCTTCTTGCTGCGCGGCAACCATGAGTGCGCCAGCATCAATCGCATCTACGGGTAAGTGGGGAGGCGGCTCCAAAAACAGGATGAAGCTTGATGAAATGGTTAGCAATCTGTTCAGTTCAGCACCGAAACAATTTGCTTTCCTGCTTAGATTACTGAAACTCATTTGATTACAATTTATATTATTCTTACGACTCGTATTTACTTCTGATGACTATGTACTTTTATACATGCTATAGAACTTATAGTTGTTTATTGTTTACGTGAGCTTTAAAAATTTCCCAGACATATTGATGTCTACTTATGCTTCCAAGCTTATTAATACTTTCTAGTTTATTGACGCACACAACGATATCCATTACAATTAAATTAGCCATACTTCAAATACATAAAGTAAATGCCACATTCTATAGCAATAATCCATAAAATTACTCAACTAATTGCACTTAATTGGCAGGTTCTATGACGAATGTAAACGTCGCTACACCATCAAGCTGTGGAAAACTTTCACGGACTGCTTCAACTGCCTGCCGGTGGCCGCCATCGTGGATGAGAAGATCTTCTGCTGCCACGGTGGCCTGAGTCCAGATCTGTCGTCCATGGAGCAGATACGACGCATC
 

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