CR02154

CRIMIC CR02154

Gene Past1

CG6148

Information

Donor Location Cytology FBti Stock number
pM37-p13R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TCCCCCATGCATTCGTTAGTGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CGCTGACTGGCCAATTATGC AATGCATGGGGGAAAGCGA AGTGGGTCGAGCCAAAGTG TTGCCACCGAGCCGTATATC
 
Amplicon 5' Amplicon 3'
CGCTGACTGGCCAATTATGCAATGCCTGGCAACGACTATATATAAACCCTATACTATGAGCGAAACATATTTGAGCTACTATATAAACAAAGTGATAAGCACCGAAAACTGATAAGACGAGCGAGCAAAAAGTCGGTGTCAATTTAGCGCTTAGGCCAGCCACACAAACGCACTTTCTGTGGCAAACTCAACATCGAGGTGGAAGAAAATAGCGCCACAAAACCACGTATTTTCTTTCCTATCTCTTGCAAATACTCTGCTACCCAAAATGTGATAGCCAATTGCTTTGTACTTGAAAAATGGGCGCCAGTGGCGGGCTGGGGCGTGGCAAACCGAAAAATTTTACTATTCATATGTGTCAATAATTATCGGCTTGCTTTCGTCATGGGTAGGAACTCAGCCAAGTTGACTGTTTTCTCCCCGCAACGTGATAAGATTCGTGGCCATGATGTAATCATTGGAAAATACCGATAGCTTGGAGCAGTGCAGGGTTCGTTCTAATTGGGCCATGAGTCATCAGAGAGGGGAAAAAAGAAAATAATAGCAGGCGTACAGTTGGGCATGATAAATATTTCACACTCGAGTACCCAGGCTGAATCGGATCTGGCTCTCCGGAGCAGACGCATCCAGGCAACCATGACGAATACCACGCACTCGATGCCGAGCCAAAACGCCCAGCAATATAATCGCATTTAAGCGTTGAGCATTCGAGGAGGAGCAGTAGGAGGTGGTAGTAGTAACTCAGTTAATCAGAGTCTAGAGGCGCCAGTCGCATTGCAGTCGGAAAAAAGTAAACCCTAATTGAAAGTCTAATTCGATTCGATGCAAGCTGGCTGCTCCACCGGTTCGCCCTGTTACTGTGACCTCCGACCTTCGTGCGTGGCCCAGAGTTCAAGGCCCAGTGCCGCGCACCTGTTCGCTTTCCCCCATGCATT AGTGGGTCGAGCCAAAGTGGCCTGTTTGTCAACCCTTATCGGGTTGCTAGGCAACTTCTAGTTGGCCGTACTGCACGTTACCCTAGATAAAAATCAATGTAAGGCCGAGTCAGCATGCCATCATCAAATCATGTCTTATCAGTCGACAAAAGTCTGGGCATGTTCCTCTACTAGCACTGCTCTAATTTCAAGCATCAAGTATGCTCATTTAGTTTGTTTATCTGCTCTTACGCTGTTTAGCGTAATTAGGCAACAAGTTGCGGCTACTGGCCGTGCAATTTTTGGCATATTTCAAACAATAAAAGCCGAAATTATCAGAACTAGTGCATATGTATATTATGAGGGGCTCAAAGGTTGTCCACCGTAAAACTTGTAATTATATTCAGCTTTAGGGATCATTGCTTGCTGAGCAAGCAACTTATATGGAAAGCCAGTAACTAATTTGATTGCTCATTTATGTAATCATCCGTTTTGCATGCACAAACCTTTTTTTATCGTGCGAGTAGAAGGCACTGTCAGCATTCTCTTGCCATTTATCAATGCTCCTTATCATGCCAGCTAATGGTGTTGTGCCCAATGAAACACTATAAAACTACAACGGAAAGGCGTGTGATTGGATTAATTCAGTATACAAAGCCGCCAGACAAGGAGAACGAAAATAATTATCTTCCAATATGCCAGGCAACTTAGAATTGGAATAGTAAATATACATATGTATATATACATTCCAATATGTGCTGCCTCCGAGGAGGAGAGATTGTCGTCCAGCTGATCAAACAGCACCCAGAATAATCCCCACGCCCAGTTCGCCCAGACGATCCCTGGTCAACCCATCGCCACAACCCACCTCTGGCGATTATATACATATGTACGTATATATGATCTGTAAACCGAACCGGTTGCAGTCGCATACGAGTACATGTATTGCTGGCGTTGGCAACCTGAGGCCGCGTATCAATTTGATGCTCTCCGATTGTGACTTGGGGCGTGTGGCACTAAGTGGCTCATCCTCCGTCTAATCCGCTCGTGAATTCCACGAAGGGGCCCACGGAATTCAGATGAACTAGCGCTAAGTGCAGGAAGAAAGCATTTTACTCTATGATATACGGCTCGGTGGCAA
 

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