CR02152

CRIMIC CR02152

Gene Nsun5

CG42358

Information

Donor Location Cytology FBti Stock number
pM37-p13R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GGCTGTCATTGATTCTCCCGTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCACGGAGTAGATGCATCCC GAGCATCATACGCAGCAGGA AGAATCAATGACAGCCGGGG CAAGAACGCGCACACTTTGA
 
Amplicon 5' Amplicon 3'
CCACGGAGTAGATGCATCCCTTGTTCTGCATCACATTGCATATGTGCACTGTCTTCATACCGGGAGCTGCGCACATATCCAGCACAGTGGCCCCAGACGGCGGAGCAAGTAGTTCGGCGGCCAAACAGGTGGCCTTGTTCTGCAGTATAAACCTCTTGCTGTGCACCAACGGATGGCGGACCCAGTAGTTGGACCACTTGGCAGGAAAAATGAGCACACCCTCAACGTGCAGATCGGTCATGAACTCGTTCTCCGCCAGCGATTTGATGGCAGTCAGAAAATCCGCATAGCTGGCGTCCGCAGGCAGCTCCTTGCGCCGCCAGTCGCTTTTATGCAGGTAGTCCAAGGCCTCCGCCAAGCTGTATAGATTTGTGTTGATGCGCACATATCGAGGATTGGGTTCTGTGTGGGCAAACAAAAAGACGAGTTCAAAATTGTAGCCAAAGTAAGATTTATTAAGAGTTGTTAAGAGTCGTTACTGGTCCTTTAAACGATCGAAATAATCTGGACTTGGCTGGCAGCACTTAAGTTACTGTTCTTTCTTTGGCTCCGCAGCTTCCGGCTCGTCGTCCTCATCCTGCTGCACGGGTTCCAAGGGTGGTGGGGGCTTCTCGCGGAAAGCCTTTAATACATACATGGCCACCACGAGGTTAACAACCACAACAGCCGTGAGAACTGACCAACAGTTGACCGTGAAAAGGTCCAAATGGGGGAAGGACTCCTGCAGCCAGCTGCGGACGCCATAGAAGCCCAAGAAGGGCAGCGTGAACATGAGCACACTGTAGGCCAACAGCCAGAGGAAGACCTCCGCACTGGTGTCCTCCTGCTGCGTATGATGCTC AGAATCAATGACAGCCGGGGAATCATCAGTGGTAGCTGGCACCACATCGACATGCTGTTGTTTCCTGTTCTTCTTGCCCATCTCAGCCGGCTAATACTGGTAATCGGTGTGATAACCTGCCTTTTCGCTGGACACCGAAATCTCTGATGGAATTTAGCAGTCGATCCTTGTAACTTCGCACCGTTTGCACGGGTTTGCTTTCGCCGTTCAGCTCCTTCCTGCCAAACAGCAGTTCGGTGACTAGAATCTTGGCCAAACTGGGATCAAAACTGGGATTGTCCCTGAGCAGTCCTGTCTCTTCGATGGCCTTTTCCAGTGCCACACGATTTTCGCTAAATTTTTTCAGAACCGTATGCAAAGAGCGTGTACGCTACAAGTGAAGTGCGAGAGGGAAAAGTTATACGCATATTGGTTTGATCTGCGGACGAGAGGTCCAATCCACTTACGGCATGCTTCTCTGCAAATATTAGGGTCTTGATGCACTTCTGCTGCTCCAGTGCCGCCTTCAAAATCTTGGCAGTCGCCCTGTATTGGGTGGGAACTTTTATGGAATGCGGTTTTTTACTCATTGTCGTCACGTGCACGTGTATGTGTTGTTTGCGCTAGAATAGGTGCAACCCCAATCACGACTTTATCGATATATGCTTATCGAATGTCAGGGCTGGAGATAAGCATGTGGCGTTTGAAACAAATCATATTGTTATTCATGTTTTGAAAATATCTACAATTAATGTAAAGGTTGTTCTAATTTTGCCAACGATAACTACAAAACATATATGTTCTATCCAATATATTTACATACAGGGTATCAAATTTACAAATAAATTGGCAGTGTGGCCATACGTTGCGAGCAAAAATTACTGTACTTCAAGCATTGCCAACACAAGAATATTTTCATGGTTAAAAATTTTGGCGCGATAAAGCCGCAGTTTAAAATGTCAAAGTGTGCGCGTTCTTG
 

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