CR02149

CRIMIC CR02149

Gene mthl5

CG6965

Information

Donor Location Cytology FBti Stock number
pM37-p13R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ATGGCCAACCAACTTCGTTGTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATTGGCCACATCTGCATGGA CGAAGTTGGTTGGCCATTCA TGGCAACTTTTTCGAGCGAC AGCACGGTGATGGAAAGAGG
 
Amplicon 5' Amplicon 3'
ATTGGCCACATCTGCATGGATCCACAATTTGGAATCCCGATGACGAATTTGAACTTAACGGGCCTGTTTTGCTCTCCTCCATAGCTGGTGAACATCGGCTGGAAGTAGTCTGCAAATGGAAACGGACGATAAATAGAGAACACAGACCGTGGAAAATCATAAAGGCAGTTCAAGTTAACGCTGCTTTTGTTGCGGCTGGTGGATCAGAGGGGAGCCAGATTTTCTTTTCTCTCTGCGAATATAAATATTTACGCTTCTGGGCAAAGAACTTGAGCTGTTCCATGCGCCTTCATCAGCTGGTTTTGCAATTTGTGCAGATTGGTTCTTCTTCAATTATCGGAGTTCGGATACATTTTTTTCCACTCAGCCATTCATATGTTAATCATAGCTAAGCACACGGAAATTAGATTGCATGTTGCGATTATGTGCCCTGTTGTATTGTAGTATTGGGATTACTCAAATGACTACAATTAAAATCGGATGAGATCAGTACAATGCAATGATTCAACTATCAGTAATAGTTACATTTTTATGTGTCACCTATATGGCCGATCCATGGTCATAAATGTTTTATCCAGTTTTAGTTGTCCGAGCTAGAATATCGATATAGATGCTAGAGTACGCTTTTCTATTTCCCACAATTGTTGCGATTGTTTGTCAAACATTCACCCAAGGCAATATATCACTATATTCACCATTAGCTCCACAAACATTAATTACCAGGGCTCGCATTTATGGGGAACAGAAGCATCTTATATTACCTTACTAATGGATTTTTTGCCTCATTTTACTTCCAAGAGATAATATTCCCGATTTGGGAATCCCTCATCAAGTGGCTACTTTATAGTTTAAACAGAAACATGCCCCACAGCATAAATCATATTTCTCATCAGGATTGGGGCCACCCTCTGTTTGTTTTAAGAACTTTCTTGGGGGAGTGCGAACTGTGGAGTTGGGGATATTTAGCTGTTGGCTTCGTTCCACTGATCCACTGATAGCCAACAAATGGGCCATTTAATGTTGGGCATACATATGTTGCATGCCAGAAGGGAAAATCCATAAAGTTGAATGGCCAACCAACTTCG TGGCAACTTTTTCGAGCGACTAGGAGTTTTCCGCCTGGCCAGCTGTTGAACTTACCCGTTTCATTGACCTGCTGACACTCATGGTCCACGTGTATCTCGAACTTCTCGCAACATTTGTTGACCAGCACTAGGTTGGGATCGGAAGATAGGGCTGTTGATGATCCGGCGCTAGTGACATGACTGGTTACTGGTAAAGGGTTTAGACTAAGAAGTAATACGCAGAGCAGGGAAATCAGCAGAGCGCAGCAGCTGCATTTTTTTGCATTTTGCCCGGCTGCAAAATGCGCGCCAAGCGTTTTTACGAGCATCTTTTGCTTAGTAACACAGACACAGATACAAATACAATACACAGGTAAAAGGCAGTTGTCGTTGGTGTTTATGTGGATCTGCGCTTGCTAAGAAATGCGTCATCAAGGTGCCGGCTGTGAGCCAAGATCATGACTTGCATGCCAGAACTCAAGTAAGGCGACACTTTTTTTTGTATCAACACACCCACAAAGGCACTTTCGTCTGGGTTTCAGACTCGTATTCGGTTAATAAACAATGCGCGCTTTACTTCCTCTTTAAACAGTATTCAAAACGAAATAAAAATTGAAGAAGCAACATCAACCGATTCGAAGCGACGACAAAAAGCGAATGACTCTCTGTCCCCGGCTCTTAATATCGAGTCACTAAGGCGGCGAGAGAGTGGAGAGTTGCGAGAGCAGCGCTCAACCCCCACCAAAGAGCTCCCTCTCTCGCATTGAAAACCAGACTGAGCGTTGTACCCGAGTGCACCGACTATTCACTGCCCTGCCTGAAGGAATTTAAACCCACATATAATACCGTTCTTATTTATTTTCCGATTTAATACTTTTTTAAGGCATGCTATCAAATGTAGAAGAAGACATAGTAGGGAATGTACTTAGACATTTATGTAGGCTTACTACGGTTTCTATATGACTAAATGCTATTTCAATTTCAAGTTACTCACTTATTGCATTTGAAACAGATTGCACGCAAACAGATCTATATACTCAAAAGTAGCGTTGTATAATACCCTTTGATCCATCGAGGGTATAAAATGTGGAAGCAAGGCTTTGAATTCGCGCCCTCTTTCCATCACCGTGCT
 

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