CR02140

CRIMIC CR02140

Gene glec

CG6575

Information

Donor Location Cytology FBti Stock number
pM37-p13R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ATTTCCTATAGCGCGCCAACTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCGCAGTGACCTAAAAGCTG GGCGCGCTATAGGAAATGC TGGGTGGTAAAAGTACAGTGGT GAATAACAATCGCGCGCCAA
 
Amplicon 5' Amplicon 3'
GCGCAGTGACCTAAAAGCTGGAAAGCTGAACAACAGCAAAAACATGAAGGCCCCAAGTCTAGCTTACATAACCGAATACTTAAACACACTCGCAAAGTAATTTGTCTATTATAATTAAAAAATCATGTAGGTGCTTCATTTAGAATTATAGAACTATGAATTCCAACGAAAGTATCTTGCTATTACGAAGCAAACTTATCATTTCGAAGTCAAGATAAATACATACAGTTAAGATTCTATGCCAAATGTATCCATTAAAGTGGAACGAAAAGCGCAACAAGTGAAATAAGCCTACTTTCTGGACGATACAAACGTGTTCCTTAAACTCGAAGCCCCCCGAGAAAGAGAATGGAAATGGCTGGCAGCCAAAGAGTCGCGCTGCTTCTGGCGATATAATCAAATGTACAGTTATAGAAAATTATAAGCCAGCGACAAAGAACGTCAAGCGAAACAGAGGAGAAGAAGCAGAAAGGAAGGAAAAACGGCAAAGCGATCAAGTCCATTGTAAAAAGCAGGTGTTGAGAAAGAGAAAGGGGGAGTGTGAGGGCGGTAGTGCGTTTACCGTTATCATTATAAGCACGAAGCTCCAACTAGCTCTGTCTCGCTCCATCTTGCACAACAAAAAAAAAACGCGTTTTGTTTACGAAATGTGTGTAGAGAGAGAGGCGGCGATGAAAACAAAGAACTCACATTATAAATATTTATGTGGGAGCCCAAAAGAGAGTTTCTGAATGCATAATTAGAAAACATAAATTTAGGGGAATATCACACACATTTTTGGGGCGTGGCGAAGAAGGCCAAGCAAAACATAATCACGAAAGTCAAATAGACCGCTTAGAAAACTTAATTTACGTGGTAAAAAATCCACACATGCAAATGATAATGAAGATGCTGTAAATTGTTAGTAAAAATATGCAAAATACCGTAATATGAATTATCATATCATAGGAATATGTATGCATTTCCTATAGCGCGCC TGGGTGGTAAAAGTACAGTGGTGATCAAAACATAGCAAAAAAGAATATAAACAAGATAGGAATAAAGGCCGAGATAACAGAACTTTTGTCTAATTGATTTGTTTTTGTTCGCTTCGCTTTTCGACCCGTTTTTGGACCTGCCACACAGTACAACAAAACACAACATTTGGCGCTGCTGTACTTACGCGAATTGCGTTCGTTGGTGTGTGTGACCTTCACGACGAGTTTTTGAAAAACATCATCAACGTGCGTGCGTAGTTACTACTACGCATAATAACGCACACACACTCGCACACGCAGAAAGCTTATCAGCGAAGACGACTAAAAATAAACACGAAAATAATAAGTTCGCGCCAAAAACCAGCAGACAAACAGAGAGAGAGAGAGAGAGAGAGAGCGACGACTCTGACTCTCTCGTCTTTCTTTGATAATGCATAAGTGTGCGTGCGTTTGTGTGGAAGTGGGATGACGTCGTCGTATAAGAGCTGTGGTTTTCCTCTGGTTGAGATTGCGTTTGTCGTTGTGGGTGCGGCCTTTTCAACTAACTAACACAACTACAGTGAACTCATGTACAGTCGCAGGTCTGTGTAGCTCATTTTTTGTTTTTTTATTTATTTTTTTTTTCTGTGTCGAAACTGCAGCAGGAATAAAAACAACAATCAAGAAAACCAGAAGGCGTTGATTTAAATTTAATTGAGGCCTCTCAATTAATTGCCTTTGCATTTTACTCATCTCATATTCACTTTTCAGCTGCAATCTCTTTTCGCAAAATATGGAAAGATTTTTGGCGCGCGATTGTTATTC
 

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