CR02139

CRIMIC CR02139

Gene ema

CG12753

Information

Donor Location Cytology FBti Stock number
pM37-p13R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence CATGTCCCAATCCGTTCAGATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCCATGCAACGGATGTGATC ATGTAACTCGTCCACTGCCC ACGGATTGGGACATGTACCA AAGGATCTGAACGCGGTGTT
 
Amplicon 5' Amplicon 3'
GCCATGCAACGGATGTGATCGTCGAACAGGAACTTAGCCGAGAGCAACGGAGTGCGGTTGTGAGTGACTCCGCCCCGGTGCACGGTGATGTGCAAGCAGCGCGAGTCATCCTTATCACCCTGCACCTCTACGTCCTGCAGAAAGCCAACGAACTTTGCCACGCCCCAGCCAAGCAGCTTTGCATCCGGCTCGACGAGAATTAGCTGCAATGCGTCAATGACCAGAAAGCGTCTTTGCTTGCTGCTGTCCTTGGCCACAACGGTGCAGGCTATTAGATCACTATTGTCTAGATGGATAATGGTTAGCCTTAATATATACATACATCATCGTAGGAGGAGTATACTTACTCAGATCGAGTACGTTCTCCACCTGAACCAGATTGACTACATTTGTAAGCGGCAATAGCGATTCCTTCTCATTCAAAAACTTTTGGCACGTGCGACGCAGCAGGAAGTAAACGCGAATGGCTCGGCGTGCCTTTTCCACCTCCCCGCAGGGAAGTCGTCGCGTAAAGTTGATTCCAGTAAGTGGCGTTCCCGTGGGAGGCAGTAGAATGGTGGAGTCCATGCACAGGAATTCCACGTTCAGTTGCGCCTTTCGCATCTCGTTGTATTCGTCTTCGAACAGGTCTAGGAAGATGTCCTCCGACTTGTAGAAGTTACGGATCACCACCATCGACTGATTGCGGGCGCTGAAGAGCAGATCCTGTTGTGCGGCGGTTATGCAGCGCGAATCATCCGAGCTGGGACGGGTGAAAGTGACCAGTAACTCCAAGGCAATCTCCACGGTAATCAACCGAATGCGGGACGCTTTAGGGAATTCAAATGGAATTATTAAAGAGCTAATGATAACCGGAGTTCGAACAGTTGTACATACATGGCTGACTGGACTGGGTGATGATGTTTAACAGATGCTCAATCAAGGCTGTCTTATAACTGAAGGCGCCACGGGTTGATTTTGCAAGAACCTGCTCGAACCACTCATTCTTGATGCCTATATGGAAAGGTACATAAAGATATTTATTAAGGAGACTATGATAGATGCATACGATTGATTAGTACGGGCAGTGGACGAGTTACAT ACGGATTGGGACATGTACCAGAATGTGACATTTTCATTACACACAAAGAAAGCAATTAGAATGAAGCAAAAATGGGGTGATCAGAGCAATGGAAAGTTACACAACATTAGCCAAAAAAAACGTCAAATTGAGTTAAGGGAAATATCCATGAATATTACCTCTATTGTGCGACATAGCATATATGAGGCACAGCGACAGCAGGGCAAGGTAATCGTTTTCGGTGCAGTCCAAAGCATGCAGGACCGTGTCTAAAAACGGTTTGGCCATCTCAGCAAAGTCAGCCTTCGTTGAGGAAGATGACTTCTGCAGCAGCTGCTTTTCCTCGTCAGTTATGTTGCGCAGTTTAATCTGCTCCGCTTCAACCGCATCTGCTTGAGAGTCCAGTTCTGTGGTGGCTGGGGATGAGGACTCCACGCCACTGTCCTCGCTAAGGGCATAGCTGGACGAGCTGGACTGACCGGTGACTGTATCCAAGGCCTGTTCGAGACTCTCGTCGGGCTCGCCGAAGCCAGGCACAACCACCTCGCGGTGCTCAACGTATGAATTAAGAATCTCGGCTGCACCCTCCTTAAAAACGCTGTGGTCACCATTGAGGATAACCCAGGCCAGAGCGTGCACCAGAGGGGCATGCGAAACCACCAGGAAGACCAATGAGAGCAGGAAAAGGGCCACGATGGAGCTCACTCGCGGATTGTGCATCTCCTGTATGTCGATGTCCACATTGCGATTAAGCACAGCAGCTAGATTCTGGGTAACCACTGCCAGTGAGGGCGGGGGCGGAGCTGGTGTAAGTGAGAATATGTACAGAGGCACGAATAGCTTATGCAGCAAGTGCTCGGTTAACACCGCGTTCAGATCCTT
 

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