CR02138

CRIMIC CR02138

Gene elm

CG2185

Information

Donor Location Cytology FBti Stock number
pM37-p13R:5652640..565486183B8Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK TTGCGAGTTGATGGCTGCGAATCTCT CGACGGGTTCCTGAAACCTACAAGAT

Images

Construct Design Sequences

sgRNA sequence ATTGGATACAAGTTCCAATGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAGGTCGCTCTTTCCTCGTG TCCAATGTTCGACCAGCTAGT GCAATTTCAATTGTGCGCTTAT AGTCCGTCTCTAGTGCAACC
 
Amplicon 5' Amplicon 3'
AAGGTCGCTCTTTCCTCGTGATTGCCGCATCCAGCTAGCCTAGTTGAGAAACCGTATGGACATCTTCTGATCCACGTCGGTGCGGTCCAGAGCTTTGCAGAAGTCCTCGAACGAGATCTTGCCCTGGCAGCACAGGTCCGCCTCCAGGATCGTCCTCTCCGCAATGCTGACCAACTGGTCCTGGCTAATGTTCGCCCCGACCATCATGTGCAGGATGGAGAGCAGCTCGTCTCTGCTGATCACACCATCGTCATCCAGATCGTACATCTTGAAGGCGAACTTCAGCTTCTCCTCCCGGCTGTTGAGCTTACTTTGCTTGTTATCCCTCAGCGGCCGGAAGTGGGCCAGGACGTTCATGAACTGCCGGAAGTTCACCCGATCGTCGTTGCTCTCAGCGAAAAAGGAATGAACTATCCGCTCGCACAGCGGATTAATGGCCAGTTCCGGGATACGCATCAGATCCTCCCGGGACAAGGTTCCGCAATCATTGCGATCCAAGGAGGTGAAGCGCGAGTAAAGTCGCTCGATTTGGTTGGGGGTGACTGGAAAATAAAATTGATAAAAATTAACCAATCGATAATTAGTTTTGAATTAAATACAATTAAGATAGTACTAAAACATGTACATTCAACTGTAAATACCTATATGATATTAACTTTAATAAGCGATTTAACAGTCCGTCAAGTTAAATCATTTAATAAAGTTGTTTTTGTTGAACGACACTGTTCAGATAAATTACTTTTGCCATGGAAAACTCGAACGATACCTATTTACGAACAGTGTGGGCATGAGTCTAAGCCCGTTCGGAATTAGTTGCTATAAAATCTGGCTGATAAGAGCGAACGCCTTATCATCTTAGCTGATACTAGCTGGTCGAACATTGGA GCAATTTCAATTGTGCGCTTATCGCGCCCGGAATATTGAAATTACACGCTCTCGGACACTAGATAATTGCCAGCCCCAATATCGGTGATAGGCGTCGACTTTTGCCCCCACCCACCCCCGTCTCATATATACGTATATAACTATCGATGACGTCATGTGGGAAGTGCAGGTGCATTTGAAATGCAAATTGCTTTTGCTGCAAACCATCTGTCCATATACCGCTTATATAAGGCGGGCACAGATCGCTCAGACTCAGTTCTGCATAATTTGAACGGAGTTTTTTAGAACACGTACATATAGTTCGTAGATTTGGAATTTTTATAGAAATGACGAATCGAAACTAAGCGAATCCATTTTGGCAATCCATGGTCTTGGACCCGAAATGCTTTTAGCACTCCAGTGCGATGTTGTTGTTTGCATTGCTCGTGGCCCAATTAATTTATATCCAAGTGCGTATTTCGATTTAGTACGGCGCATTGAAAGTCTCCGGTTGAAAAAGCCCAAGGTTATCACACATCCCGACCAGAAATCGTACTCACAGCCAGTCTCCTCCTGGATTTGCGCGATCTCCTCGTTCCGCAGGAAAAGCGACGACTTATTGCCCATGGTGACGGTGTGTTGTGCGCCGATCGGTTTGTTTAAACATTCAACAATCGCAGACGAGCATTGAATGGCATTCGATTTGGATTGGACCACAAGTCCGATTTGTGTACAGGCTGAATGCAGGCGGAGTGACGGGAAAATGCCGAAAATCGGGTGCAAAAAAATACTGAACAGCCAGCGATGGTTGCACTAGAGACGGACT
 

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