CR02135

CRIMIC CR02135

Gene CG8176

CG8176

Information

Donor Location Cytology FBti Stock number
pM37-p13R:9516007..952723785D19Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK CTAGCGCTTTCAAGAGATTGAGGAGG CGCTATTTCCTTAGCAGGCTGTATGC

Images

Construct Design Sequences

sgRNA sequence CTATTGGTTCAATGGCATTATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGCCAGCGGTAGTATACCCT TGCGTGTGGATGTGCAGT ACCAATAGCACTTACAACTGCA GTCATAGTCCAGCAGCGTGT
 
Amplicon 5' Amplicon 3'
AGCCAGCGGTAGTATACCCTTTGCCGAGGATCCGATACTGGGAGCTATGGGCTCTCCGCGTCCCACATCGCCGGACATGCTGGCGGGAGCCAATGCATCGGGAATACAATCGCAACCGACTGGTGGAGCCACATCCACGGTGAAAAGCCTGCGATCCTGGTTTTTGCCCACTGCCAAACAGCAACCGTCTGCAGGCGCCGGAAATTTCGGTATGGCCGGAAGTGGCAGCACCAACAATACCACCACCACGACAACAGCAAGCAACAATAGCAACAACTTGAACACCAACAACACAACAGCAACAACTACAACAACAACTGGCACGAATAATCTTACCACAAATATCACCAAAAATTCCCCTAATTCTGAGGATACTAGCAATCAAGGTTCTTTCTCTTTGATTTTATTTCGTTTATGTTTTTGTTTATTATTTTTCATTTACATCGTTGTATGGTTAAAGTTTATTCCATGTATCTACTTGTGGGGCCTATCATTTGTTCTATCAATTTCAACTATTCTTCTGTAAGATATTTCCTTATATGTTCTTTTGTATGTATTTACGTTTATGGTCGACTCTTAATTGTACCTATTCATGTTGCCTAAAGTTTTAAATTGGTTATTAGAAATTGTGGGATCTAATGCTATGTATTAATTTCACGAGTTAGCATTAGAAAGTAGTATTTTATTCATTATAATTGTAAATAATTTTATTTATTTTTATTTTACTCATTTCTTAGTTTAATGCGATCTTTAAGTGTACACTCAAACACCAGCCAATCAATCAGACAATTCTCCTGACTTTTACACAACTACTACTGCACATCCACACGCA ACCAATAGCACTTACAACTGCAATCTTCTCTCTATGCTTTTCTCTGTCTTATTTCCGTACTAATGCTTTGCACACAACCATCGCATCCATCAACCACTGCTCATCCAAATCATCCATCATCCAATCATCCACCCATCCGTGCACCGATCGACTAATCGAAATCAAAACTTTAGATCAGACACAACAGCAACCGGCAGAGGCCTTAACTCCAACCGCAAGTGCCACTGCAATTGCAGCGGCGAGTGCGCCTGGGAAGAAGCAATTGAACGCAACGACAAGCGAGCAGCAGCTATCGAAGTCATTAAATGAGCAAGAGCACCTACAACAGACTGCAGCAGCTACGGCAGCCACAACTGCAGCAGCAACAACCGCCACAACGACAACGTCCAGACGTGCAACGTCACTTTTAAATATATTCACATCAAACTCTCAGGGTAAATGATTTAAGAATGTCAATGAAACTGATTGGCCCCCATTTCACCACCCGTCCAACTTCTAAAACACCTGTACAACATTTCCACCCGAAAAAATTGCATGTACCTATCCTTAATATGTATATTTAAAGGCTTTTCTGTGTGCTCAACTTAGTGTTCTCTCATATCCGATTAACATCTAATTGTTATTGAAGCACTTTAAGGCGGTGTTTGAGTTCTGAGAAAGACAATGCATTTAAGTTTCAACTATATATTCCCATCACCGAAGTCTCAACACAGAAGTATATTGATGTGTTGAGGTGTCATCTTCTTTTCCTGTGTATTCTATTAGCCATTAGCTTGCTTATTTACTTAAGAATCTCTACTCTCCTGCTTTCATTAATGTGATCCACCTTTCGCCGGTAGTCTCTGGCATTTTACGCAGTCGTCGCGACAAGACAAAAACCAAAAAGGCTAAGAAAAAGAAAGACAATGAGGCCGCCGAGAACATACAGGAAGAACGCCTCACCAGGTGGGTACACAAAATATTTATGAGGCTCAATTAACAAAATCCTAAACATGAACGCTCTCTTAATTATTTTCTAGTTTGGATCGAGGCAACAACACGCTGCTGGACTATGAC
 

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