CR02128

CRIMIC CR02128

Gene CG31321

CG31321

Information

Donor Location Cytology FBti Stock number
pM37-p13R:14310362..1431441288B3Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK long band AGGCATGTGCGAATTCCACAGTTCAC TCGCAATCAGCTGAAAGACAATGGCG

Images

Construct Design Sequences

sgRNA sequence TAGGCTGAGGTCTTTGGAAGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTCATGGCCTCCGTGTTCAA AATCACCCGGCAATAGCGAT CCAAAGACCTCAGCCTAGTGG GGCGTGGCTATGGTGATGTA
 
Amplicon 5' Amplicon 3'
TTCATGGCCTCCGTGTTCAATGCGGTGGCCATGCAGAATTTCCCACTGGACAAGGCGTGTCGCGTGAATCTCGGCTACAACAAGATCGTCTGCGACACAATGCTGGACAAATCGGAGCTGGGCATCGAGTGCGATGACTTTGATTTCGAGAACACCACACAGGGCGCGACGCCGGATCTCGCTGACCTTGTCATCGGAGCCACTGGCTTCAACTACACGGTGTGCAAGGCGGAACTGGAGGCACAGATCCTGGCGGCAGATGTCTCCGGGAAACGAGCTCCCATGGGTAAGCCAGGCGACAAACATACACATATATCATACACATAACACGAGTCGTTCATTAAGTATACGCCGTTTTGATTTCGGTTATGGGTTTGGCATTAGCATTGCTTATATTTCAGGTTTGTGCTTAAATTAAATTCGAATTCTTTTCACATCTCGACTCCTCTGCATTTTAAATTTCCCCTGCCCCTCGAATGGGTTAGGTACACTCGAAAAATAATAGTATTATCAAGTGATAATGGGACTAGAAAGACATTGAGATACTTTACTCAAGCATAAGATTGTAAATATCTTAGAGAACTTTCTTTAAATTGAAATGGAATAGCTATATATACACATTTTCTTTGAGTGCACATTTTTTTCTGAAGGAATCCGTGTCTTTTGATGGATGTCGTCGGAAATTAGCGGTTATCCGAACATCTAATTACCTTCTTTAATCGATTCGCTCATAAGTTTCTCCTTCGCCTTCTGTCTTCTGATGGTGCTAACAAAAATTCCCCCATTCTATCGCTATTGCCGGGTGATT CCAAAGACCTCAGCCTAGTGGATACCTAGTAATCGTAATCGCAATCGCTCCAAACCTGTTCCTTACATCGTTGTGCGCTGAGTGACTTTCGTGGGCTCATAGAGCCATTGTCAATGGCACTTTGTTTGAGCCATAGATAATAGCTTATATTCCCAATTTTTAGAATAAACGCCATCCGAATATGTCATTTGCATAGAGCTGCGCGGAATTCCGGTTCTGTGCGCCAGAGGAGATCTTCTTCTTGTTTAATAACTCAAACAAACGGCGGGTTCATTAAGATGCATTGCATCAGAGATCGATAATTGAGGCTTTAAATGATTAAATCGTCATGTCATAGTCTCAATGGTGAAAAAGTTTAGTATACATACATACGTATCAAAGGCATTCGTTGTTTTAGAATGCTTTAATTCCACGGAGATAACTAGTTTCAATAAAATTCAAGTCAGAAATTTAAATCATCTTCAATACTCGATGCCATTTTGGAATTTGGCAAAAAAACAATAGGGTTTTAAAGCGGATTTATAATAGTCTTGAGCAATAAACTCACTAAAATCTCATAGTAAAAATAAGTAAGTACTTCTAATTAAAATTTGCTGTTAGCAAACTTTTTATCTTGGCAATGAAAACCTATCATATTAACAGTAGAATATAGTGAGTTAAATTGATAAGCGACAATGTTACTAAACAGCTTATTTTCAAGAACGAGTTATTTTGGTACCAGTTCTTAATGTCAAGCAAAATTAGCATTACTAAGCAAAATAATGTTAAATCATTTTATTTTTATTCCAGCTGCCATTTTCCCGCTGATTGTCTTACTTTTTGCTGGAGGATGGGCAGATCGATACAACAAACGAAAGCCTTGCATGATAATGCCAATTATTGGAGAGGCATTATCTTTCACCTGTAAGTAGCTTAATTATCAAAAAAAAAATAATATAATTTTAAAACACTTAAATCTTAGGTCAGATTATCTCATCGATTTTTTTTGAATCGCTGCCAATGGAGTTTGGCGCCTACTGTGAGGCTATAGTTCCAGCTCTCTTCGGAGGATTAACCTTCTGTTTGATGGCAATCTATAGCTACATCACCATAGCCACGCC
 

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