CR02127

CRIMIC CR02127

Gene CG31064

CG31064

Information

Donor Location Cytology FBti Stock number
pM37-p13R:27229505..2723908597E10Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band (weak) OK TTGCCAAGCACGCTCCTACTTTGTAG CACCAATCTGTTTGGCAACTACCGCA

Images

Construct Design Sequences

sgRNA sequence ACTCGGCGGTACTACTACTCCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CAGTGCAAAACGACGCTCAA AGTAGTACCGCCGAGTAGCA GTCGCTCCACCTCACAAAGA AAAAGTCGTTTCCCAGCTGG
 
Amplicon 5' Amplicon 3'
CAGTGCAAAACGACGCTCAATACGAACGGAAATGGGTGGCGAGCTCCGGACAAGGATATATTGTCGCAGTTCAAGGTACGAAAGATCGAACCCGTCAAGCTGCCCAAGAGCTGCTATTCCCAGGAGGATGCCTCCAGCTGTGCTCAACTGGATCAAACATGCATTTCCCAAGAGCCGGATCAAGGGAGCATGATTGTGAGCCTGCCTCGACTGGAGACGCTCGATGAGAATGGCTATATGGGCAACATATACTTGCCCTTTCGAAAGCGACGCATCTACAATCTGCGTTCTCCCAAATCGGAATTTGTCGTGGTGAAGTTCTTCCAAACCCTAACCAACTGTCATCGGTTTCAGGGCATTAGCCAGGCTAATTACAATCGCCAGTTGCTCGCCTGGATAAAGGAAAATCTGGAAATGCACTATGGCGATGATGTATTCTACCCGGGTCTGGGCGGTATTCTGCAGATTCAGGAGAGGCTGTCGAAAGAAGCGAAGAATGCGGAGGAGGAGGCCCGGGCGCCAACCAATAAGAATGACGGGCAGGGATCACCATCCTGTCCCAAAGAGGACTCCCGCAAGATGAGTCGCTCCGCTATTCACTGGCAGGAGGCTTCAGGGCCAGTCGAGGAGTCGGGGCCGGAGCCAATGCACCAGGACGAATGGCTTACCGGATTGGATAAGGTTCAGAGTCGGCCGGAGGAAGTGGAAGAGTGCCGCGAGTGCGACAACGATGAGACACTGATCTGGTGCATTGCCGCCTTTCTGACCCTGCTTCTGCTCCTCATTCTGCTCATCATTTGCTGTTGCATGCGAAGGGGCAGGAAGAAGAGGGTTGCCCCAACGGATGCAGAGGCTCCTCCGCCGAAAGAGAGGCCAAGAAAAGAAAAGGAGCCGCTGAAAGCCAAAGAACCAGAGAAGGAAAAAGAAAAAAAAGATGAAAAGGACGAAAGTGATGCTACTCGGCGGTACTACT GTCGCTCCACCTCACAAAGATCGGTGCCGAGCAATCGTAAGTCAAATAAGTTTGTGGCAACTAAATCAACTAAATCTAACTGCTCGAGTTTATAGGATATGCGAATAATCCCGAGCTAGATTCCTTCACCTCATCTTCGTCGCTGGGATGTCAGTTCAACAGGAAGTGCATGCCACTGAAGTTCTCCCGAGCGAAAGGAGGGGACTACTACGCACCGGACAAGAGCCGAGATGCGGTGGGAAAGCAGGTCACATCCCTCAAACTTCTTTCCGATGATTCGTCTCCTAGCCGAGTTCCCTACAGGGACGTTTGTAAGCCCGCTCTAAAGTCGACCCTATCCGTCATTCCCTCGTCGGACAGCGAAGTGGACAGCAGAGAGGATAGACATCGACATAGGCATGCCCGCGAAGAAGACAGGCGGAAGAAGCATAAGGACTTTGAGACGGGTCGTAAGAAAGAGGTAAGTTATCAGTGCTTCGCGTGCTTTTGATATCCTGAACTCAGAGTCACCAGCAGACCAAATCCTCAGAAGACAAAAAGCAGCCATCACCAGAAAGGCGATTGTCCAAACAAGTGAGGATAGGTAAGTGCATGACTGGTCATTTCTTAAGCCTAATCGATTCATTCACTTAATCCTCTGATGCTGCCATCTTAAAAGCAACACCGGAAATGTACAAGGATACATCCGATGATCCTCTTCTGCACAGGAAGAAGTCGCAGGAGAAACCAGATGTCCCTGCCGGTCGACAGAAGGGCGGTGCAGAGGTGAGTGGGATGTGCAAAATGTTATATGCCAAAATAATGATCTTGACGGTTTTTCAGTATTTAAAGGACTCTCCCAGCTGGGAAACGACTTTT
 

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