CR02120

CRIMIC CR02120

Gene CG11000

CG11000

Information

Donor Location Cytology FBti Stock number
pM37-p13R:5802328..581325883C4Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band long band TGGTTGATGCAGTTCGTCGCATGCAT AGCGCAGTAGATAGCCTCCCTTGATT

Images

Construct Design Sequences

sgRNA sequence CAAACTGATTACGACCCGAGTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGCCAGGCCAGCAATTATGT ATTTGCTCCGCCTGGGAATG GGGTCGTAATCAGTTTGCTGC GAGGCCAGTCACACGATACC
 
Amplicon 5' Amplicon 3'
AGCCAGGCCAGCAATTATGTCTGTACACGTTTGTCGCTGTCTTCGCCTTCAGATTCGTCCTGGCCCACTGCGAACGGGAATCCGAGTGTGAAGTGGGCCAGGTTTCGTTGTCTGTGGCAGGCACTTGCAATTCTAGCTGATTTGTCATGTGGATGTGGATGTAGTTGTGAATGTCGATGTGTGTGTGGATGCAAGGATGCGAGCATGCAGAATGCCCACATGCAAGTGCGGCTGTGTGTGCGCGTGGGCATGGCTACGTGCGGTTGTGTGCGTGCAGTGAAGTGGCACACATGATGTCCGCTGCCCTGCCTTCTTACACAAGCAAGGCAGTGTCCTTCGGCGAGCTCGTTCTCACCCCAGCAACCCCAACCCATGCAACAAACATGCCAAGTCCCTTGCCCCTGGCAGCCCCAGTTCCCAGCTCATCCTTTCATCATCTCCTTCGACACTTGGACTGCGTCTATCGTAGTCGTCTGCAGTTCGCTTGCTTCTGCTACACTTGCAACATTGAAGAAAGTCAAAAGACTGTCAACTATATATGTTTTTAAGTAATATACTCATAACTCGTGGAGTAAATAATAAACTTAAATCATTGAAAAGTATTTTGAAGGTAGAATGAAGCCCCCTTTTAGCCAGCAAATTGCTAAATCCTGATTTCTTTCAGGACCTCCTTTAGCTGAATAACAGGTATCCGATTGCTTCTGCAGCTCAACTACACCATTCTCTCTCTTTTATCATTGACCAAGACCACTAGGCCACATATATTTCTCAATGTGTATGAAAATGGCAGAGGTCGCAGGGAAAAGAAGGGCAGATGCAGACCATTTTGAGGGCAAGCGCCGTTTTTTAGCACGTCGAGCCTCGAGCGTTTTTCAAATGCTGTGCGGCCTGTGCAGGCCAATCGCATTCCATTCCCAGGCGGAGCAAAT GGGTCGTAATCAGTTTGCTGCAAAGGCAACTTTTCGAATGGTCGAGTGCGGAAAAAATGCCAGAGAGGTCCTTCAATTCCGAAATTGCAAGAATATCACCCGAGGCACATTTAATCGCCTATCAGATTCTGGGAACAAGTTCAATGCTGGGAATTTATGAGTTATTGCTCTTATGAAATCATAAAAAGTTATAAAGTTATTCTATATTTTTGCTGATTTCTTCAAAAATCTTTATATTGTTTATATATTTTTATTGTAATAAAAAAAACCCTTTAAGGGTAAACATAATCCTACCTAATATAACTTGTTGGTGATTTGAAACTGATGGTTAAATATTACTGGCACTAGTATGGGATTTCCTACTTTTTTGAATGAACCCTTTTCCACACATATTGAGTTTAAAGGCAAAAGATATAAAGATATTCGTTCTTACTGTTACACAGTTGAGCAATATGAACAAATTAAGGAAATTAAATTTAAATAGAATTCTACACTTTATGTTGAACTCGTCGCTGGTGGCAAAGGTGTGAAATCGCTTCGGATCGGTTGCCTTTGCCATCTTTATTGAAATACTTAATTCAGTTCACCGCAAGCGTTGTGTTTGTCACTACCCCAAATCCTCCCACTCCTCCGCCTTCTCTGCCTTCTGGCGCCCACCTGCGACTCTCGATACTTGACCAAATAAATGGGATGCCAGCGGTAATGAATTTGAAATGATCGACCTGGTCCAGCCAGCAGGAGCGGCGGCTGGCTGGCTGATTGAGTTGCTGGCGATGCAGTTCCGGGGGAATTGCTAAATGAAATTCCGTGTTTCGTTTTTCGCCATTCGTTATCCGGCGCAGCAGTCGCACATGGACTCGCCCACGCTTGAAATTTTCAGCTCCTGCTTCGGTGCGTGGGCGCTTGGTCCACGACTGTCGGCTGTCGGATGTCGAATGGCGACGAAAAGGAAAGAAGTCCTTGAATGGAAAATGGGCTCCAGCTGCAGACCTCGATTCCTGTTCAATTAGTGGTATCGTGTGACTGGCCTC
 

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