CR02110

CRIMIC CR02110

Gene CG5447

CG5447

Information

Donor Location Cytology FBti Stock number
pM37-p03R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK long band

Images

Construct Design Sequences

sgRNA sequence TGTTATACGAGTTTAAAATAAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGGAACGATAGGGATGGGGT AACTCGTATAACAGATAGCACGA ATAAGGCAGTGGCACTTCCA TTTGCACGCCACATTTTGCT
 
Amplicon 5' Amplicon 3'
TGGAACGATAGGGATGGGGTAACACTATTTTGGTATAAAAGCCAACTCGCCATCTGGTAACACTTGGAGTTTGCAAACATTTATCTCTCGACCAATTTAAGGAATTTGAGGCAATTAGGGCATTCCTGTGAGCTTTTCCAGGCCAACTGCGATTACTTCAAGCCAAGACAAACAGGTGGGTAACCAAGCAGGCCGTGCCCCACCGACCAGACCAGCAAAACACCCCCATTTCGGGCGGTGCTGGAAAAAGCAAGGCCAGCTGTCCATTTGGGATTTGGACACTCCACTCTGAGCATCAAGATGATTGCCCTTAACCAAATGCATCGGGCGCTGCAGAGCGAGGCCATTAATGCCATTATCCGCCTTTTCAGCGACAACATGTCCCAAGGCGGTGGCACTACTCCAGCGGGCGGTAATCCCACAGCGCTCCAGGCGACCGGAGGGGGCGTGGTCTTCAGCAAGGTCTTCATCCAGCGCGATTACAGCGAGGGCACCTCCGTCAAGTTCCATACACGGCTGCCCGCTGAGCTGGAGGGCATGGTGACTATATAATACTCTATATTATCTGTGGGATTATCATCCGAACTCATATCGATTCTCGTGCAGATTGAAAGACACGTCTTCGAGGCCACCATCAATAGGCTAAACGAGTTCTACGCCGAGGCGGAGGAGGGATCCTGCGGCACCTATTGCGAGGGCTGCATTGGCTGCATCACGGCCTACTTGATCTATATGTGCTCCGAAACGCACTATGAAAAGGTGCGTCATTTGGGTTTGGATTACACACATGTGGGTCAATGCAATAGAGTCTAGTTCCACTAGGGAACTATTAATAGGGATTAGAGTCAACAATTTCACAAATGTCGATATGCGATCGTATAAGTTATCAAAGTTATTCTCGTTAATGTCCTTGTACTCAAGGTTTTCATATCTGTTTAAGGTATAAAGGTCAGAGAACTTGATAAATCAAGTAGTAGTATTACTTGCTAATTAGTGATCGTGCTATCTGTTATACGAGTT ATAAGGCAGTGGCACTTCCAGCTCTATTGCCTGCACATACTAATGCTCTTTCGCCCCCACAGACCCTTCGCAAGATATCCAAATTTGTGGCTTCCCAAAATGAACGCATTTACAACACCAAGGGACTGCAGCTGATCGATCCCACTTATCGGGGACTTCGCGTCATAGAGATTACAATATTCGATCGTCCGGGGCGAACGTGACTTCCGCTTTCCCATTCAGCCAACGAGTTTTTCATGTGAGATCGAGCAGCTACAGTCGCATCAACAGGCATGTGAAGGAGAAGAGTCCGTTTCAGCAATTATCAAGCAAAAGTCAGCGCCATCAGAGTGCGTTTCCAAAAATATAAATCAATAAAAAGCTGTGGAAGCAGCTAAAGCTTTATAACCTATAACCATATGTGTGAGTATCTCAGATATTCCGCACTCAATTGCAAACTGAACTAACTAACATTTACTTTCGCATCCTTTCAGCTTGGCAAAAGCAGTGGCCAGAGCGTTTTCGGGGCATGCGACTTAGTCACGGGTGTGCTGGTAGATGTGCCTCCGCTGCAGCCACATACTGTATGCTAGATTAGCCGTTAATGATTAGCCAAGGCAGTGTACGGCCAGTTAGTTAGTATTTATGCAATAGCAGGCATAGTCATGTAAGTGAAAAGCTACCGAAACTGCATTTTGAGCCGAAGCAAGAAGCATCAACATATACACACATTCGCATATGTTACAATTGTTTTATTAATCGGACCGAAAGGCCAGCGAGAATTGATTGTTTTGTAATTTGAAAATGAATAGAATTGTATACATCATGTTTGTAGTAGTTGCCAACAATTCGATTGTCTAATGTTGTAATTTCAATATGGCATTAACATTTGCATAAATACACACACACATAGTTCAAGCTTAATCAGACATAATGCATACGCATAACTTTTTCTATACAATACTTACCGATTTCAAACAGCGCCAAATGAACTTACACATAAATCGAATCGAGAATCGTTCGATATTTAATATAAATCAGCAACATTGATTAACATAGTTATATATCTTATAAGAAAAATATATATATTGCCGTCATTTAAACGCGCCTACAACTGTTTTCCTGCGGCGGGTAAACATCGAATTGGAAGCAAACAAGTTAAGCAAAATGTGGCGTGCAAA
 

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