CR02103

CRIMIC CR02103

Gene CG1129

CG1129

Information

Donor Location Cytology FBti Stock number
pM37-p03R:4816025..481144482D6Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GACAAAGTGCAGTGCGAACATCGGAA CGCAGCGAGCTTAGATACAGACACAT

Images

Construct Design Sequences

sgRNA sequence GTAACCGTCTTCCCTAAATTTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACCGGAACATGCCTGATTGT GGCAACGGCAACCAATATGG AGGGAAGACGGTTACATAACAC ACTTGGTTTCTCCCATCGCC
 
Amplicon 5' Amplicon 3'
ACCGGAACATGCCTGATTGTGGTCTTTAGATAGGTTATTTAATTTAAATTAAACTGAAAAGTATTTTTTAAGATTCTTCAATTTAACAAGAAATGAATTTTAATAAATTTTATTAATTTAGCGTAAAATGTTCGGTGGAATCCGGCTATCAGCGCTGCTGCGAGTGGGACGAAGCGCAATCATCGCCATCTTCCGATAGTGTTATCCCAACTTTGCCAGCTCGGTTTTTATTTACTTTCCTGTGCGGCGAGTTTACTCATAAAACAAATGATAATAAAATACACAAAGACCATATTGAATGTTCAATGACAAGATCGATATTCCTAAGCCTTGAAAACGTCCATCTAAAACTAATAGAAACTATTATTGTTTTGGGACAGGCAAGTGCATCCAACCAGAAGCACAAAGGTTCTTATCACGAAAAAAGACAATTCCAAAAACAACAACAAGGAAATAGATAGCGCAACAAGGTGAGTAAACAATAAACTAAAGACAACCAACTGAGTCACTCACTTGCCTTCGTCGATTGCAGTTGTTTGACCGCTGAAACGGAATCTCGTTGTACGGAGAAAGTCTCGAGAACTTTAAGGTGTGACGTGTCGCTCGCTTACGGACTATAGAAACCGGAGAACGGAAAGGAGGCGGAAGCGGAAGCGGAGAAGTGATGTGTGGAAATCCAGCTGTGGGAAACGGGACGAGGGCGTTGATTCTGGTCGGTGGTTATGGGACCCGGCTGCGACCCCTGACCCTCAGCACGCCCAAGCCACTGGTGGAGTTTGCCAATAAGCCGATTCTTCTCCACCAACTGGAGGCACTCGTCGATGCGGGATGTCGTCAGGTATATCCAGTTTTTTAGACTAATGACGCCCAAACTGCCCCATTGCTTAGGAGAATGGAAATATGGCACATCATTCTTAAAACAAGGTTTTTTAAACACCTTTGGATGTTTAGAAAACAGTTGATCTTGTTCCATATTGGTTGCCGTTGCC AGGGAAGACGGTTACATAACACGCTAAGAATCAAATCAACTTCCTCTAAAAGAGATTATTCTTTTGTTTTATATTTCTAAAATAATGCATACTTCTTTCTCGCAGGTTATTTTAGCCGTCAGCTATCGAGCCGAGCAAATGGAAAAGGAGCTTAAAGTCGAAGCCAAGAAACTGGGCGTGGAATTGATCTTCTCACACGAGACGGAACCCCTGGGAACAGCTGGACCTCTGGCCCTAGCAAAAACGATTTTAGCAGCCAGCTCAGAGCCATTTTTCGTGCTCAATTCGGACGTTATATGCGATTTTCCATTTAAACAACTAGTGCAATTCCATTGTAATCACGGAAAAGAAGGCACAATTGTTGTCACAAAGGTCGAAGAACCATCCAAATACGGAGTTGTGCTGTACGATGAGAACGGCTGTATAAAAAACTTTATTGAAAAGCCACAAGAGTTCGTTAGCAATAAGATCAATGCCGGCATTTACATCTTTAATCCCTCCGTGCTTGACCGGATCGAAGTTAAGCCCACATCAATAGAGAAGGAGGTGTTCCCTGAAATGACGCAGCAACAGGAGTTGTATGCAATGGATTTAACTGGATTCTGGATGGACATCGGACAGCCAAAAGACTTCCTAACCGGTACGACAGACAGAATCACTCGTTTGCTCGCTCAATCTTGATTGCCTAATTGGTCTTATCGCTTTGAAAGCAAATGTGGATGTCGTTGTTAATTGTGCTGTGCCTTTTTACCCACCCGGCTTTCGGCTTTATCTATTATTGACATTCCTGGAACTATAGCTAATGTTTGATAGAACGATTTCTTTTTAAATGAAGTGAGGCTCATTAGGCCGATAGTGGGCGATGGGAGAAACCAAGT
 

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