CR02101

CRIMIC CR02101

Gene 5PtaseI

CG42283

Information

Donor Location Cytology FBti Stock number
pM37-p03R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TGCTGAATACCAATTTGTTTGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACAACGCCTTCAGTGTCACA ATTCAGCGCCCGCCGATAAT TGGGAACTCGGATTCGGATAT ATTTCGGACAAGTGTTGCGG
 
Amplicon 5' Amplicon 3'
ACAACGCCTTCAGTGTCACATCTAAAATTAAAATCCCCAAAGAGGAAAAATGGCACAGTGCCATTCTGTTCGTCCAAATGGAACCTATTCAGTGTATATTAACTTATTATTAGCTAAAAGATTAGGCAGCAGCAACAGTCACCTTTCAATGGTATGAACTAGGGCTCTTCTGCGCGTCTTACAATAAACGGAGGGGAAATTTTCGCATGCAGCCAAATTGGATGCATCGTGGAATAAGTGAATGTTGACTAGATCGATGACGGTGCCATTGATTTCCCATCGGGTTCGCATAAAACCTTTTCGGGACCATTTACACTGAATAGAATATAGTAAGAATATGAACATAACTTCATGAATTGCATCAAACCTACCTCCGGAAAGAAGTGCTGTGGGAACTTGGACTTTTCTTTGGTAGCAATAGTTTCAATATTTCCGGAATAGATATGTTTATCCTTAACATCTTGAAGACTTTCTTCCCAGCTGTGAGTTAGGAAGTTCCAGATCTTCAGAGAAGCAACATCCTTATGCACAAAGTAAAGACTTCCAAGGGCCTAAAAATAATAATACAATAATGTGGGTATTTGTTTTAACCGGATATAACATTTTTGTATGTTGATATGTGAATTCAAGAGACTCATTCATCATGACTAAGCCCAGTGACTTAATTCATATTTTTTAAAAGGCTTTCGTCGCAATTTTAATTGAACAATAAGTGCGTAACGCCTCAGTTCATCCGAATTGGGTTTTTATGTGCCGATTGACCTAGAAACACGATTCACCCACCCACCGTAAAGTGCTCGGCCGATTTGAAGTCCTCGTCCATGAAGATGTGTACGCAGGTGAAGTCGCCCATTTCGGGAGCATCGCAGAGGCACCTGATGAACTCCTGGACGTACTCCATGGACTTCTCGTACGTCTTGCCGCCCACCTCCTGTAGGTGGAGGGCCAGGAAACGGGGCTGTGCGACGGCGATCTTTGCAAGAAATTCGCTTAGCCATAGCTGCAGAAGCCTCTCCGGCTGCAAATTGGATTGGAAATTCGATTATCGGCGGGCGCTGAAT TGGGAACTCGGATTCGGATATACATATCTATGTATGTCACTGGGCCCATACATAAATATGCGATTTACGCATCGTGTATGTATGGGCCGTACGTAAACATGTATACTTCCTAGCCCTGTGGACATGGCGCATCCGTTCCAACGGCCACTTACGTCCTCAAATAGGCTGCCAACGTTTGCCGTGACCAGAAACACATCCGTGCTGGACGCGTCTTCCTGTGCTGACTCCATTTGATCCCACTTTCGCCTGCGATGCAACAATTTTATTCACTTTTTATCAGCGTTCCAATTGTAAATACTAATTGAGTCAAGGCGCAGTGTGACCATACCGCCTGGCGAAAACTAGGCCATTTCGATTATTAGCTTTTAAGTGACCGTAATCGTTTATATTTCAAATATATTTATGCATATCTCTGCAGTTTTTTAATTAAACTTATTTATTTCGTTTACATTGAATAATACAATACCCCTCACGAATTTAAATAGTTAAGCTTCATTTCTTTCATATTTTGTTAACTTTAGGTTTCAGAATCGTAATTAGGTCACTTCCGGCAGTGACCGAGATAAAAGTAACGGTAATATCATGAGTACAATGGCCAGTTAAAAGTAGATATAAACAATTAAATGGCACAGGGAATAGAAATTAATGGGTTTGGACAAAGCAATAACATTGAAACTTGCAGGTTTCTAATGTTTTACAGTTCTTTGATCCTTAATATTATTATTTATCCTATTATCTTGACTAAGTTTTTGAATTTCAGTGTAACTGCAATTAAATGCGTATAAATGCAGCTCGAAATTAACAATTCATGCGGTGTGGTGGCACATTAAGCAATTTGGTGATCCGTTGTCTATATTATAAGCATATCCGCCGACATAAAACTATTAAGGCCCATTAAGACCTACGGTGTACATCATCTTTTCATTCAAACAGCTTACAGTAGTTACTGCGTACTGCGTATGACGGTTTCAATCTTTGACAGAATTCCTAAGTATCTTCAAAAGTATTACAACCGCAACACTTGTCCGAAAT
 

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