CR01903

CRIMIC CR01903

Gene ATP8B

CG14741

Information

Donor Location Cytology FBti Stock number
pM37-p03R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AAAGCGGCATACATACATGGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TAGTCCTTCGCCGGCAAATT GCTTTCACCCGACCCGAATT GGCGGAAACATATAAGCAACAC TTTCGGGTCCAAAAGGCCAA
 
Amplicon 5' Amplicon 3'
TAGTCCTTCGCCGGCAAATTTCTATGAGATGGAGTGTGCCACAAATCAAATCGGGGTCAGGAAAATCATTAAGTTCTCTCACATTAAGGTGGTCCTGGGTGCGCGCCTTAAGGTCCTCCTGTCCGCCATGCAGACGATCATATATCACATTGTCCGCTCCTTTGCAGTAGAGAACCATGGAGTCACCGCGCCGGAGAATCACAGACATCCGCTTGCGGACGTTGTTAAAGTCGAGGATATTTAGGAGTTCGTACTCCTATAATATTTTATTTAAATTATGTATATTTTTATATGTATAACAATTACTCACCTCCGTTTGTCCCATCACCTCGATGGTAATACTATTCGGTGTTCGTGTGCGAAAGACAAAGCCAAAATTTCGAGCGGCCGAAACGAGGGCAGCTTCGTCGGGACTTTGGGCCTGGTACTCCAACTTACCATCAACCGTTTCGGCCATGACCGTGTGGCAGAGGGCCAGGAGGCGGAAAAACACATGGGAGTGCTCTTCATCCGACCGGACGGCATCCAGCAACGTGCGATCGTACCAGCGGAAGTCACTCTCGTGATGCGGATTGGCCGAAAAGTCAACACTTTGAAGGGCCTGTGAAAAACAAATAGTTTGCTTTACACTCGGCGAAGATGAAGGCATTTCTGAGTTACTGTACGATAAGCTTGGCTGTATACAATTTTAAGCCAGGTTAGCATAGTGTCCCAAAGTATATAATGCTTAGTAAATTATTGTGGTGTTACAATGGAAGTATTTACGATTCCAAAGCGGAAAATTTTCTCTTAAAAGCGATACGCAACTGGCTTCAACGTTGAAACGTTGATCATGGGTAGAATATGATGTAAAATACGGCCTCATGTTACATAAAGGTAAAAACAAGTAATGAGCGGTACTTTTTACATTATAAACATAAAAACGTTTCTTAAAAAATAAATATTTTTATATTTTATTTGCACAATAACGAATTCACGACTCTGCTGTGGGTATATTGTGTGTATCAGAAAAAAGGGTTGTGTACATCATACTTTCAAAATAATAAATACGTTTTATCGTGTTAAAGCCAAAAAAATTCGGGTCGGGTGAAAGC GGCGGAAACATATAAGCAACACATAACAAGATACTAATTGATAATAGTGAATGTGAATTGAAGGACATGCAGCATGCATACGTGCACTCTGTGAGGCGACGAACTCCAATTGGTGGCGAATGCCTTGGATCGATGGTGGTGGTGCTTGGTGGTGCTAAGTGTGGATGTGGATTGTGGCTCCTCGTCGAGGTGGTGCTCGAGCTGGGAGATGACAACACCCAGCGTGGCCGTTGATGGCGCTTTTTTAAACCGGATCTTGACTAATCGCTTGCGATTGTGACTTGAATGGTGCATGTTGTGTGTTTCTGTCTGTTGGCTGTCATGCAAAATCTTTACTTTGTCGCAACTGCTAGTGCGGGACAATTGGCGCTGCATACATCCGGCTCCACTCCTTTTGAAGCCGCCACTAATTCGTCGCTCCTCATTGCCTACGGGTGGACTAAGTCCTCCACTTCCATCCAACCTGGGTGACAAACGACCAGCGTCTGCATCCTGACTTCTACTGGGAGCCGAGTATCGCACATGCTTGAGTCCCGGCCGCTTCTCGCTGGCGTCCATCGGCGGAGCAGAGCGCTCGATATCCGATCTGGGTCTGTCTGGCAGCACTTGCGCCTCCCCGCTGGATGTGACCACCATGGAAGTCTTCTTCGCATGGACCTCGGCCTTGTGCACTAGGATGATGGGGGGTGGTGCTGCAGGTGGTGCTACGATAGCTCCACTTAAGGGGCTGGGTCTGTTGTTGGTGTTGCTATTTTGGAAAATTGTTTGCTGCTATAACGAGTAATGTGAGTTGTTGTTTCTTCTCTTTTTTTTTCTTTTCTTTTTTACATTGTCAGACACACAATTAAACAAAGACCCACACGGTTGGCGGGTCAAGGGGGAATGTCATAGTTGAAGTTTGATTTGAAGAAACAGCCTCGTTCGAGACTGAAATTTTTTGGGCAGACTGTGCAAGAAACGGACACCGACACACACAAGACAACATTCCCTCAACCAAGATAGAGGTTAAATTGGCCTTTTGGACCCGAAA
 

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