CR01899

CRIMIC CR01899

Gene CG3376

CG3376

Information

Donor Location Cytology FBti Stock number
pM37-p22R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AAATAGCTTTAAATATCTAATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GATCATATCGCGCTATGAGA GGCTTAAAATATGTGATTGAT TGATCGTCATTTCGCATTCTCAA CGCATGCATGTGCTTTCCA
 
Amplicon 5' Amplicon 3'
GATCATATCGCGCTATGAGAGCACAGTGACTGCTCAGTTTTATGGACACACGCACTACGATGAGTTTGAAATGTTCTACGATCCGCACGATCTGAGTAAGACCATATCCCTTGGGCCAATATCACAGGACCCAAATTTACTCCCTCTTTTCAGACCATCCCAACGGCATTGCGTACATAGGTCCCTCTGTATCGCCCTATTACGATCTGAACCCCGGCTATCGTATCTACTACGTGGATGGCGATCATGATGCCACGACGCGCCTGGTCATCGACCACGAGTCCTGGATAATGAACCTCAAGGAGGCCAACCTGTATGGCTACCCCATTTGGTACAAGCTCTACACAGCCCGAGCGGCATATAACATGAAAGCCCTGCGTCCCAGTGATTGGAACAACCTGCTCAACGAGCTGACCAATAATCAGGAGCTATTCGAGCTGTACTACAAGTAGGTGTTTGTGGTCAGATTGAACTAAATGGATTCTATACAGTTATGGGTTTTCTTTCAAGATACTACTGGAAGAACTCCCCGGCGCGACCCACCTGCGATGCGGAGTGCAAGAAGAGGCTCATCTGCGACTGTCGGAGTGGGCGTTCCCACGATCGGAAGCACTTCTGTGCCGAGGTCGAGTCGAAGATCGACGAGGAGTCTTCCAAGTCATGGAAGTCCTGGTTCTACAAGGGATTGACCAATTCGTAAGCACTGTTTATGTCAAGTTCGTGTTTCTATGGTGTAGGTGGCTCACACAAACTAATGCAACTCGAGTTGTAATTGTAATCAATCACATATTTTAAGCC TGATCGTCATTTCGCATTCTCAAAACATACTATTACTGCATAATAACAACCCCATTTTATTTTTCCCACTGGTGTGGTCCATGCAACAACTAAAAACCTAAAACATATGACCGAACAGCGCCGAGGATCGGACTGAAGAGCCGGCCCCCACCACCGATGGCTATGGGCCTTTCGATGTGGTGATTGTTAACGTTGGTGGTTGATTTACTAAGGAATAAGGTCCCTGTACATATCACTGGTGGCCAGAATCTGTAGTGCCCATGACAATAAATGACCTAAATATTTACCCCCAAACTCTTTCTTCTGCCCAAAAGCTATAGCCTTCTGTCCTCCATCACATACCTGCCCAAATACCTGCTCGGATATCGCTGATTCCGATCTGAGATGGTCGTTTGAGGTCATCCAACTGCAAGTCAATGCAATCGCAACGAAGGAAAACTCCTACCCCATAAGCTTACTATGTGATAACTAGTGCTTAAAACCATTTCGCTTAGTGTACGTTAACGCAATGATGTCCCAAAACTGCCTGCTTTGAAACCTTACAATCGCCCTTCGGAATCTGAGTCAGTGAAGAATAGTCATTTATCTGTAATCGAGTCGATGCTGTTGCCCACTCCGCAGCATTTGCGGTCGTGGCATGTTGCGCGCTTTTACGCAATGGAAATTGTCGAGCAAATTACCTTCTAAGACACCTAATTGTAAAGCTAACCTAGGTCTTAACTTAAATAAAGTGCAATTATTTTTATAAAACATAAGATGTTTCATCTCTTTGGCGTTTGTTTTCCCCGAACAAAAACAATATTCTCCATTAAGATATAAATTCGAATTTAAATTCAAATTTACCAGTGCTGGAAAGCACATGCATGCG
 

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