CR01896

CRIMIC CR01896

Gene scyl

CG7590

Information

Donor Location Cytology FBti Stock number
pM37-p13L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK

Images

Construct Design Sequences

sgRNA sequence GCAACGTTAAAAAAGACGGAGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACAAATGCAACGGACCAACG GAGGGCCAGAGAGACGAAAA TTGCCTCTGCTTCACGTTTG GCTCTCGATCATCACAGGGG
 
Amplicon 5' Amplicon 3'
ACAAATGCAACGGACCAACGCCATTTAAGTGATACAACAAAACAAATAAAAGTTTAAACAACATAAGTGCTGTGATTTCCACTACAAAACAACAACCAACCAACCAACCAACCAACCAACAACAACAACAACAAATGTTAATTTAAACTGCAACCAGAACGTAAAGAGGAAGAATTTAAGCAATCAGCAAGAAAACAAACAGCAAATTGTGATAAATTAAATAAGCTTAATTAAATTGTAAAAGTGATATACAAAGAAGGCGACAAAAGCCGTCGGAAAATTGCGAGCGAGCGAGCGAACGAACGACTTTCGATTCGATTCGACTTTCCAAGCCAACAAAACACAGAAAAACCGATGAAAATGGATGTTATTGCTCGGGAACAAATCATCTACGGTAGCCTGCAGGGATCAAACAAAAATAAAGGTAAGTGTACCGAAAATTCGGAGAGAAATTTGTGTCTATAGGGAAGAATCGGGAAAGTTCCAGAAAGATTTTAAAACTTAAAATAAAACATGTGTTTCCCAAAATGCAGTCTACTTTTATCAAATTTGTGTATAAAGCGTACATATTTTTAAATGTTTTTTGAATACTTAAACTGTTCTTGAACTTGTGTATAATTTAAATAAAAAAAAAGATGAACAACAACTGCTGTACAAAGCTCCTTGAAATTTCATCAGCAATTAACTTTGTAATTTACATTTACACAAAAAAATGCATAAAAAATCAATTTGCAATTACAAAAAGGAAAAATGTCATAAAAAATCATGTTAAATTGTTCGTCACGTACGCAAATTGCAGCAACAACAAAAAACTCCCAGCAGCAATAAAAAAATGATTTTTCGTTTTCTTTTCGTCTCTCTGGCCCTC TTGCCTCTGCTTCACGTTTGCGTGCTGCGCTTTGAACTCTCGTTCGCTGTTGCCAAGCGGCTGTTTCCTCATTGCCTAGTTGTTTGTTTTTGGCTTCCGTTGCGTTATTCCTAATTTTTGCCAGATGTAGCAATAGAATTTAAGAGGTGTTTTAAGATTGACTAGTAACATTCGTCACTACATTTTTACACTCTATGAAACTTAATAGTATTCTACCTCGTTTTATTACCAAAACCTTTTACTAATCAATCGTTTTTCCTCCTTTTCAATCATTCCAGATTGGACCAGCAGACTGCCACCGCCGTCGGCATACACTCTGGACCTGATGTCCAAGAAGGCCAAGACGACAACCGGGGGCAGCAGCAATGGCAGCAATGCTACCGCCACATCCACAACCACATCCACATCCAGCAGTATTAAGCACAAGCAGCCGGCGGGCAGCAGCAACAACAATGTTGGCCAAAGTCAGAGCAAGAAGACAAAGCCCAGCGGCAGCTATAATAGCAATAGCAACTACTACTACTACGCTGCTGACGAGGAGGAGGGCGGTAGCGCTGACTATGCACTGAGCAACTACGATAAGAAGGCTGTCGAGGAGCTGTCCCTGCGATTGCTCGACGAACTGAGGGCGGCCAAGTCGCGTCATTTGACCTGTACGGAAGTGTCGCTACCCTGTGATCTCACGCCGAGCGTGGCCCGGGAAATAATCCGCGTGTCGGAGAAGGAGCCCCGCGGCATACGCGGCTGCACCATCTACATTGAGTTCGAGGATGAGCCGAAGAATTCGCGTCGCATTGCCTCGATCAAGGTCGATTCGGATACGGTGTCCACATTCGAAGTGTATCTGACCCTCAGGCAGGATCATCGCGGCTGGACGTCGCTGTTGCCGCAGTTCATGAAGAGTTTGGCCCGCACGATCACCATCAGTCCGGAGTATACGATCACCAAGAATAAGCTATATTCCGCTGATGGTTTGGGTGCACGCAGGAGCTATAGCTTCGGCAGCCACGCCCATAGACCCAGTGCGGCCATCGCCACGCCCACCAACTAATGGTGATCAGCTTGATCTGTCAGATATGCATATGTTCGTATATGTGTCTGCGCGAAACAAAAAAAACACCACCCCTGTGATGATCGAGAGC
 

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