CR01894

CRIMIC CR01894

Gene scramb2

CG1893

Information

Donor Location Cytology FBti Stock number
pM37-p13L:3046812..304411363A3Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CAGGCTCCTCTTCTTCAGAACATAGG CCAGTCCGGACCATTGTTTGCTGATT

Images

Construct Design Sequences

sgRNA sequence CAATGGCGGTCAGGCGATTAGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACAGTCAGGCATGGCTAACC CCTGACCGCCATTGTAGCTA GGGCCAGTGATAGCAGTTCT GAAGGTATCGCTCACGTCGT
 
Amplicon 5' Amplicon 3'
ACAGTCAGGCATGGCTAACCATGACGTCACTGCCTCGGACTGCAGTCTCTTTGCTTCTGTACGATCACAGCAGCGGTCAGTAAAAGGGCTCATAGATCGGCACAAACACTTGGTGCATTTTAGCCATAAAAAGTATAAATTGATTGATTGATTTTCCTATATCTAGAGGCATCTGTTAAATAACGTATACAATTGAAAATTTAAAACTAGTGTGGGTGCTATTAATTTGAACGCTTCTGTCTGTTCATAATTATTTAGAATATATGTATATAGGCACTTATAAATGTTTAATAATTAAAAAAGCAATTAAGTGGACGCCTGATACTGTTCATTTTGGAATTTTATAAAGCTTCGTCTTTGACTGATGAAGACCATACATATGTACTCCTCCGAAGAATTTAAAGCTAATCCGCTGACCGCTGCTGTGTGTGTATACACTTGTTTGGGCATTGGGAATCGAGATCGAGAGCCAAGAATAACCACTTGTTGGCTGCGTACTGACCTTGAAGTTGGTATCGCTGAAGCACTTGCAGGGACAGACGGGTCCCTCGATCTGCAGGACGGTATCGCCGCATGTGTTCTTGATGTTGAACTTGGGCCGCATGAAGGTGCAGACCTGCTCAACGCTACCGATCACCTGGCCGGGTGGCGCCGACACCTCCACGGCGTTCATGCAGCTGGGAAAGCAGCAGAGGATGTCGCACTTGAAGGGCCGGTACAGGTGGAGCACCTCGTTCTGAAAATTGTCCAGTATCTTCATCTCGAAGGGGCGCGACCGGCCGAGCATGTTGCGGGTGCAGCAGTCGCTCTCCTCGTACGCGAAGTACACATTCTGGCCCAGTGAGTTCTTCACCTTGAAGCGGTTCTTCGTCTCGAAGCCGGTGAGCAGCTCCAGTTTCTCGATCTTCTGCGACACGAGCAACTGGTCCAGAGCGGTCAGGTATTCCAGTCCCTGCGGGCAGTTGGGCATGCCCACGGGTATCGACATCCAGTTTTCTGCAATGGGGCGTTGTTCCGGATTAGCTCCCTATGTATTTATATTTATCTATATGCATAAAGTGCTTTTCCGATATCTATGCATGGGTAAAGGAAATTAGCATAGCTACAATGGCGGTCAGG GGGCCAGTGATAGCAGTTCTTAACATAAACTAATATTTTAAACAATATGTATTTACGTATTGACTTGGAATCACTTCACTTTTTGGTACATTATAAAATATCTTTTTTACATAGATAACTATGGGTGCTATTGCCATGCCCGCTGCTGTACTAAATGCCAAGAAAGAGACGACGGCATTTCCGCCTTTCGCACACGCCCACTAGCCCACACAGACGCGCGCAACCGACTAGGTCCATCCGAGTGATGGAATTCAACAGTGGACAAATCCATATATGTCCATCGCTACGCACCAGGTCCGTTGGCATTGTTGCCAACATTCGCCGCCTGCGGCTGCAGGACCACTGCGCCATTCTCGTCCTGCGGTGCATTCTGCGGGAGCACCTTGTACTCGCTCATCTGCATCATTGTTATTCCCAGCAATTAAATTCGTATCGGATTCTTTAACAGTTCACAAAACACTAAATGCAGGGTGACCGCGTTGCGAACGCCGAAAAATGCAACGCAAATGCACCGTACGAAATATTCTGTCATATATTTTGGTATTTATTCGAACACAGAAAAATATCAACAAAAAGCTTTCAGCACTCAATGCAGTGACAAGTTAAATTTGTTATGAGCTGACGTTTTTGTGGATTCATTTGTATTATTGCATTGTATTGCAATAGTTTCCTTAAGCTAGCCTCTTTAAGTTCTAAGAATTTTGAGTTTATTTGTGCCAGTTGGTATATTCGCGCCTGCCACCGACGGTAACACTGCGGCTATGATTGGATGATAAGCGATCCATAAAAGCCTGGACACAAAAGACCTGAAACATGCTGCTTGGCAGCCGAATCTCCAGTCGAAATGAGCGAATCTCTTGATAAGACCACCAGCCCGAGCTCGGCCAGTTCCAGGGCCACGCCCCCGGGAACGCAGGATGCGGATGAGGGCCACGACGTGAGCGATACCTTC
 

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