CR01889

CRIMIC CR01889

Gene miple2

CG18321

Information

Donor Location Cytology FBti Stock number
pM37-p13L:275018..27120061B3Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GGAGCTGGTCATTGAAGTCAGAGTAG TTCTGCTCCTCCTCCTCCTCAAGTTT

Images

Construct Design Sequences

sgRNA sequence ATTCCCATAAAAGAGCAAGGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATGCGCTCTTCCCATCCAAA GCCCGATTCTACTACGTTCCC TCATTGCCGGCTTAATGGGT CTGAGGTTCACACGGAGTCC
 
Amplicon 5' Amplicon 3'
ATGCGCTCTTCCCATCCAAAGGGCAGCTTGTTTGGCCTTTCGGAAGTACGTTCTTCAGTTAGGTTCGATAGCCCACTGTCCCCATCACCATAGGAGGCGCTTTTCACGTTAACACTCGAATATACGCTTTTAGAGCTCGCGTCAAAGCAACCCCAAGAGTGGTCACAAATCTGATCTCCATTTTTGCCATCTTCTTTTTTAAATATATCAAACTTGCATAGCGCCGGATTCTTGGAGTTCATTTTTGCACAAACTAGACGGTGTAGGTATTTATGTGACAAGTGTAGGTACTGAATAGGTTACTGCTTTGAACCAAAGTCTTAAAGAAAAATAAAGCAATGCATTCTTGTTTGTTTTACAGCTCGGCTTTAAAGCATATATTTTTAATTATATTTTATTTTCATGACTTTATTGTTTTTAGGTTTCCGAAATAGTTATGAATCTTATACTGGCTTTAACCACGGCTCTGCACCTAGTTTTGGTGGTATTGGCGCACAATCCGCTATCAACTCGTGGCGAAAGCCTTTCTGATGGCATACGTCTGGTCATGGAAAAGAGGTCGGGCGATGTGGTACACATTCGTCCAGCTCGGGGCACACTGGAGGCCGAAACAGTCGCATCCACAACAAATAGTGACACCCACCGCACCAGGAACAATCGTAAGCCGAACAAGCCTCAAGAGCACTCTCAGCAGGTTGGGGCCCACAAGTCGGGATCTAGAAAACTAGTGGTTGCGGAAAATGGCGGGGCACTGTCATCACAACTACCACAACCAAATCACAAGCAAGGCCACAAGAACACTGATAAGCAACAACGAGGAGGGGGATCCAAGCAGCAGCGAGGACATAGCCAACATCATGGAGGAAATAGGAAAGGGCCTAAGGCAGCGACTCCAGAGAATGGTAAGCCTTAGGACTGCCACGTCAGAATTCAAATAGTTTTTAAATGCAAAGTCTTATATTTGCTTGTTAACAGGATCGACTTGCCGATACGCAAAGAGCGCTTGGTCCAATTGTGATCACAAGACCAACATGCGTTCACGAGTCCTGTCCCTAAGGAAAGGAGAGCAAAACTGCTTGCCCACGCGGACCATACAAAAGAAGTGCAAGAAAGGTGCGCGCGGTAACTTCTATATGTATCATAATCCTACACAGCGCCTCCCCAAAAGGGGAACGTAGTAGAATCGGGC TCATTGCCGGCTTAATGGGTCCAAAGGTCACAAAATGCAAAAATCACTCTTGTTGACCCATATACAAGAATGCTTCCTTCTGGGGAGGATTGTGCTCTCTTCGACCCACTTCGAGGTTCTCCCCGCCACAGCTTCGTTTCGTTTCCTAGGAAATACTGCAGTTCCTGTTCCTAACAATGCAGCGAGTGCATCCTTCATAATAGCAAATACTTTCCAAAATTAATTTGAAATTTTGCGTACTTCCTTCGGTAGGCTGCCGCTATGAAAAGGGTGAATGGTCTCAATGCGTCGGAGGACAAATAACCAGAGAGGATAAGTTGGAACCAGAGGCAACTGGCGGTAGTGATCAAAACTGCAATCCTGTGCGTACCGTCAGCAAGAAGTGTAAAGCAAATGGCAATTCCAGTGGAGGAAAACAACACGGCCAGAGTCGTCGAACAAAAGAGCAAAAGCAAAAGGATAAGGGTAAAAGTGGTGAATTTGACCCCTTTGTGAAAAGTTTGATCCAAATTCTCTGTTTACAGGAGCCAGTCACATTTCGTCTTGATAATGAAAATTATTTATTTTATACGTATACGGAAGCAACATTTTTAATATTAAATAAGCGATTTTTAAATGCTAGCCCGTACGGACTACTTGAACAACAATAAAGAATTGGCCTTCTTAGGGCTAAATGAAAGCACTCTTTTAGAAATTCTTGAAACAAGGCTTTCACAAACCCCAATACCGCGAAAACCTGCACAAAATGAGACCTGTTATTGAGTAATCAAAGAGCATTGATAGAACTGTTAGTTGGCTCTGAGTTTTTATTTAATATTAGACGCGACAGTAAAATAACCGTCTGCGACAAAATTTAATTAACATTACACAAAAGAAAACGTTACCTCTAACTCGGCTTGGTAATATAAAGATGTTAAATTTAATAGTGTTTCTTTACTTAACTGCAAGCCCCCTTTAAATTGATCGATCTTTCGAGGCAAATCTTCAAGTTACCAATGTCTTTAATAGCCATTGAAAGGGTGTTATGTGGCTGGCCCAAGATTTGCCGCAAACTTATTGTAACGTCGCGGTCGCTGACCTCCGGCTTGCGGCGGGCACTGGTCAAGCAGCCCCGTAAAGGCGGAGATGTTGGAAAACCCGGAATGGAGCTTGGGCGCTGTTCGTGCTTTGGACTCCGTGTGAACCTCAG
 

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