CR01881

CRIMIC CR01881

Gene SPR

CG16752

Information

Donor Location Cytology FBti Stock number
pM37-p0X:5446656..54940124F11-5A1 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GAAGACGCGACGCTCATTGACCTAAA ATGATGGTCTCGGTGGCTGAAACAGT

Images

Construct Design Sequences

sgRNA sequence CATGGGCAGATAAACTCGCCTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCTTTCCCAGCTTTTCCTGC ACAATGAAACGGTTACCCCCT GCCCATGTCGCTGTAATTGC TGCTTACCTTTGAACGGCGA
 
Amplicon 5' Amplicon 3'
GCTTTCCCAGCTTTTCCTGCCCTTTGCACGTTTGCCTTGATTAATCAACATTTTCAATTTATGGCACTCGAAGGGCGCGGAATGGGGATTCTGGGGCTTGGCTTGGGGGGTTGGCAAAGCAAAACTGTTTACTCACTTCAATGGAGTAGTGTCAAGCTGATAAAATGCCCACCCCCATTTGGTCATAAGCATAAGCCCTCCTCAGACTCTGGCATAGCCAAAGGCATAGTGATTTACAGAAGAAGGCTATGGAAAAGCTCGGGTGTTGAGCGGAATTAATCCATTTCAGTGTTGGATTTTCCAAAAGAAGATAAGCCAAAAACATTTACCTCAAAAAGGTGATAGACATTTGAATAATTAATTCTTTTTAAATGGAATTAAAAAAGAATCAGCAGTGCTAGAAACTTCAAGTCAATGAGAAATATAAAAGTACTTATGGTTTTTTTTTTTGGATTATGACCATTTTGCAAGCGCTCTTGCCCCACTGTACTGGCTCATTGTAGCTACGTGCCGTTAGTGGACTCTCCGGGGATTTGTACCAGCTAAATTCCATGCTGGGCTGGATGTCTAGATGGATTCCAAGGGACGGGGGCCAAGAATTGAGATGGCTGCTTGGCCTGGTTGTGGCGGTGGTGGGGTGGTAGCAAGGTCCTTTGACTATCTCTGCGTATGACTGCCATAAAATTCAACTCACATTCGCTGGAGCGGAGCACTCCTCGTTTTGGTAATAACCACCAGGCGGCCATTGCAGTCTCAAAGTTGAAGATTCCTTTTCATTTCCACCAAAAATTTATATGCTCGTCGGCAGCACGTCCTGGCCCGTCTTCATTCCGGAGATCTCCATGGCGAGTGGTGCGCATAATGACCATGTTAATGTGAAAAGGGGGTAACCGTTTCATTGT GCCCATGTCGCTGTAATTGCACACACACACACACACGCACACGCACACATAAACCGGATTAGGCCACAGATTGTGCATTATATGGCCAAGAGTCCGATGTCAGGCCCACAGTATCTCGCCCATGGAGCGCCTGTCTGCCGCAGAGCCGCACTCATAATGGTCCATAAATGGAGCAGACACCTGTCCAGGTGGCCATTTGCCAGCTACCATTTGCCAGTTACCATTTACCAGTTACCATTTGCCAGTTGCCAGGTACCAGTTACCTGGATTTCAGACGCCACGCACGCCATTCCATTCTATTCCATCTTACTGCCGGCTAATGCAGTTGAGTACCACAAATCCGATTTGGCAGACAGGCTAAGGAATGAGTTTCTGTGCCCTTGGGCCAAATGCTGATTTACTTACCTATCGCAGACATATATCTTGCTCAATTCTCATGGGTATAATGGGTTCGTATGATGGACATTAAAAGATTTGTTACTTTTTAATGGCAAAATATAAAAGGGATTTTGAGCAGTATTCATTTGAAAGGATTGCATGAAATTTAATGTAATAGGTAAATATTGTAAGATCAAATCACATATAAATCAAAGAACTATGATTTAAGTCAAATAGCTGAGAAGCATGCCCGAAAGTTGGCTAGTAAACACACACAAACGGCGCTAGTTTCATCTGCTACCAGCAAACCCTCGCTTTCAGTCATCTGTAACTATATATAATATATATATTTACCTGTACTTTTGCTTACAGATAATGCCAGCCATGTGCCACACCATCTCCGTTTGGCTAACTCTGGCCCTCGCCGTTCAAAGGTAAGCA
 

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